公开(公告)号：AU2012216605A1

公开(公告)日：2012-09-20

申请号：AU2012216605

申请日：2012-09-03

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： RANK DAVID R ,  WEGENER JEFFERY ,  KORLACH JONAS ,  ROITMAN DANIEL ,  XU YUE ,  LYLE JOHN ,  TURNER STEPHEN ,  PELUSO PAUL ,  OTTO GEOFF

IPC: C12N11/00 ,  C40B40/04

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

公开(公告)号：DK2002019T3

公开(公告)日：2012-06-18

申请号：DK07754529

申请日：2007-03-29

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： RANK DAVID R ,  WEGENER JEFFERY ,  ROITMAN DANIEL ,  XU YUE ,  LYLE JOHN ,  TURNER STEPHEN ,  KORLACH JONAS ,  PELUSO PAUL ,  OTTO GEOFF ,  CICERO RONALD L

IPC: C12N11/00 ,  C40B40/04

公开(公告)号：AU2006330947B2

公开(公告)日：2012-04-12

申请号：AU2006330947

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： BIBILLO AREK ,  MITSIS PAUL ,  PHAM THANG ,  CHRISTIANS FRED C ,  MURPHY DEVON ,  PARK INSIL ,  HANZEL DAVID K ,  LYLE JOHN ,  RANK DAVID R ,  CLARK SONYA ,  PELUSO PAUL ,  OTTO GEOFF

IPC: C12Q1/68

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

公开(公告)号：CA2991818A1

公开(公告)日：2009-10-01

申请号：CA2991818

申请日：2009-03-27

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TRAVERS KEVIN ,  OTTO GEOFF ,  TURNER STEPHEN ,  HEINER CHERYL ,  MA CONGCONG

IPC: C12Q1/6869 ,  G16B30/00 ,  C12Q1/6806 ,  G16B30/10

Abstract: Compositions for nucleic acid sequencing are provided that include template constructs that comprise double stranded portions in partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.

公开(公告)号：AU2008331824A1

公开(公告)日：2009-06-11

申请号：AU2008331824

申请日：2008-12-03

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： OTTO GEOFF ,  EID JOHN ,  KORLACH JONAS ,  HARDENBOL PAUL ,  FLUSBERG BENJAMIN ,  ROITMAN DANIEL

IPC: G01N33/53 ,  G01N33/535 ,  G01N33/573

Abstract: Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.

公开(公告)号：CA2991818C

公开(公告)日：2022-10-11

申请号：CA2991818

申请日：2009-03-27

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TRAVERS KEVIN ,  OTTO GEOFF ,  TURNER STEPHEN ,  HEINER CHERYL ,  MA CONGCONG

IPC: C12Q1/6869 ,  C12Q1/6806

Abstract: Compositions for nucleic acid sequencing are provided that include template constructs that comprise double stranded portions in partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.

公开(公告)号：CA2707600C

公开(公告)日：2018-02-20

申请号：CA2707600

申请日：2008-12-03

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： KORLACH JONAS ,  ROITMAN DANIEL ,  EID JOHN ,  OTTO GEOFF ,  HARDENBOL PAUL ,  FLUSBERG BENJAMIN

IPC: C12Q1/00 ,  C12M1/34 ,  C12M1/40 ,  G01N33/53 ,  G01N33/573

Abstract: Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.

公开(公告)号：ES2648313T3

公开(公告)日：2017-12-29

申请号：ES16157104

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： HANZEL DAVID ,  OTTO GEOFF ,  MURPHY DEVON ,  PELUSO PAUL ,  PHAM THANG ,  RANK DAVID ,  MITSIS PAUL ,  CHRISTIANS FRED ,  BIBILLO AREK ,  PARK INSIL ,  CLARK SONYA ,  LYLE JOHN

IPC: C12Q1/68

Abstract: Una composición que comprende: una ADN polimerasa recombinante, comprendiendo dicha polimerasa recombinante una secuencia de aminoácidos que es al menos un 70 % idéntica a la SEQ ID NO:1 y comprende una sustitución de aminoácido en un sitio correspondiente a K512 respecto a la SEQ ID NO:1; y un análogo nucleotídico que está marcado en un grupo fosfato, en el que el resto de base del análogo se selecciona del grupo que consiste en adenina, timina, guanina y citidina.

公开(公告)号：AU2015202111B2

公开(公告)日：2017-03-16

申请号：AU2015202111

申请日：2015-04-24

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TRAVERS KEVIN ,  OTTO GEOFF ,  TURNER STEPHEN ,  HEINER CHERYL ,  MA CONGCONG

IPC: C12Q1/68

公开(公告)号：AU2015202111A1

公开(公告)日：2015-05-14

申请号：AU2015202111

申请日：2015-04-24

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： TRAVERS KEVIN ,  OTTO GEOFF ,  TURNER STEPHEN ,  HEINER CHERYL ,  MA CONGCONG

IPC: C12Q1/68

Abstract: Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.