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公开(公告)号：KR102096629B1
公开(公告)日：2020-04-03
申请号：KR1020180084217
申请日：2018-07-19
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12Q1/689 , C12Q1/6893 , C12Q1/6895
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公开(公告)号：KR1020170079564A
公开(公告)日：2017-07-10
申请号：KR1020150190278
申请日：2015-12-30
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12Q1/70
Abstract: 본발명은오제스키병바이러스의백신주및 야외주감별용프라이머세트및 이를이용한감별방법에관한것으로, 구체적으로백신주와야외주에존재하는유전체서열에특이적으로결합하여중합효소연쇄반응을통해특정크기의증폭산물을생성하는 3종의프라이머세트가포함되어증폭산물의생성여부를통해백신주와야외주를감별할수 있는프라이머세트및 이를이용하여백신주와야외주를신속정확하게감별하는방법에관한것이다. 본발명의프라이머세트를사용하면, 오제스키병바이러스의야외주와백신주를매우쉽고정확하며빠르게감별할수 있다. 특히본 발명의프라이머세트는국내에서발생한다양한야외주뿐만아니라중국에서발생한바이러스주로부터도백신주를감별할수 있어, 이후중국에서바이러스가유입될경우신속한대처에큰 힘이될 것으로기대된다.
Abstract translation: 本发明baeksinju和室外主差动底漆为伪狂犬病病毒设置，并且涉及使用该方法的判别方法，特别baeksinju来的一定大小的扩增特异性结合通过聚合酶链反应，以基因组中存在的外周序列 包括三个的引物组产生的产物涉及的引物组的方法可以来baeksinju来baeksinju，并通过使用这一点，使外差快速，准确地辨别在是否产生扩增产物的周边。 使用本发明的引物组，可以非常容易，准确，快速地区分Ozeki病病毒的开放区域和疫苗株。 特别是，引物对本发明的可以做到这一点，以及各种在国内的份额robuteodo baeksinju病毒引起室外主差发生在中国，预计将立即采取行动后的主要力量，如果一个病毒在中国推出。
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公开(公告)号：KR101642727B1
公开(公告)日：2016-07-27
申请号：KR1020150191298
申请日：2015-12-31
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N7/04 , C12N15/85 , A61K39/12 , G01N33/569 , G01N33/535 , A61K39/00
Abstract: 본발명은약독화된돼지열병바이러스생마커백신주및 이를이용한경구투여용백신조성물에관한것으로, 보다구체적으로는, 기존의돼지열병바이러스백신주 Flc-LOM-BErns를세포간교대연속배양하여얻은약독화된돼지열병바이러스생마커백신주(CSFV BErns attenuated C-P50), 이를포함하는경구투여용백신조성물, 및돼지열병바이러스야외감염돼지와상기약독화된돼지열병바이러스생마커백신주접종돼지의혈청학적감별방법에관한것이다. 본발명에따른약독화된돼지열병바이러스생마커백신주 CSFV BErns attenuated C-P50은종래의돼지열병바이러스백신주의문제점인임신모돈에서의유사산등의부작용이완화되었으므로안전성이강화되었다. 또한, 본발명에따른경구투여용백신조성물은미끼제형으로제형화되어돼지가손쉽게섭식할수 있으므로, 주사제로만사용되어성질이사나운사육멧돼지및 흑돼지들에게백신투여가어려웠던종래의돼지열병바이러스백신의문제점을해결하였다. 또한, 본발명에따른혈청학적감별방법을통해돼지열병바이러스야외감염돼지와, 상기약독화된돼지열병바이러스생마커백신주 CSFV BErns attenuated C-P50로접종된돼지의항원및 항체를감별할수 있어 DIVA(differentiating infection in vaccinated animals)의효과가우수하다.
Abstract translation: 本发明涉及减毒猪瘟病毒活标记疫苗株和使用其的口服疫苗组合物。 更具体地，本发明涉及通过在细胞间连续和连续培养常规猪瘟病毒疫苗株Flc-LOM-BErns而获得的减毒猪瘟病毒活标记疫苗株（CSFV BErns attenuated C-P50） 包含其的口服给药疫苗组合物; 以及用于血清学鉴别猪瘟病毒室外感染猪和猪的方法，其中接种减毒猪瘟病毒活标记疫苗株。 根据本发明，减毒猪瘟病毒活标记疫苗株，CSFV BErns减毒C-P50减轻了孕妇猪中假酸的副作用，这是常规猪瘟病毒疫苗株的问题，所以 安全得到加强。 此外，将本发明的口服疫苗组合物制成诱饵剂型，因此猪容易消耗。 因此，已经解决了仅用作注射剂并且难以在激烈的丛生猪和黑猪中施用的常规猪瘟病毒疫苗的问题。 此外，通过本发明的方法，可以观察到猪瘟病毒室外感染猪和其中减毒猪瘟病毒活标记疫苗株（CSFV BErns减毒C-P50）的猪的抗原和抗体， 因此，在接种动物（DIVA）中区分感染的效果非常好。
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35.发明公开
公开(公告)号：KR1020140034342A
公开(公告)日：2014-03-20
申请号：KR1020120094251
申请日：2012-08-28
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K38/162 , A61K9/0019 , C07K14/01 , Y10S424/813 , Y10S514/867
Abstract: The present invention relates to a vaccine composition comprising domestic predominant epidemic genotype strains of bovine viral diarrhea virus for preventing or treating bovine viral diarrhea. When preparing a vaccine by mixing GB44-1 strain, GB45-1 strain, and 08Q723 strain which are the bovine viral diarrhea virus according to the present invention, the vaccine can be valuably used as an effective vaccine for preventing or treating the bovine viral diarrhea since the vaccine is suitable for domestic genotypes and has an outstanding immune function compared to a conventional vaccine, and solves a problem of a conventional vaccine for the bovine viral diarrhea.
Abstract translation: 本发明涉及一种疫苗组合物，其包含用于预防或治疗牛病毒性腹泻的牛病毒性腹泻病毒的国内主要流行基因型菌株。 通过混合根据本发明的作为牛病毒性腹泻病毒的GB44-1菌株，GB45-1菌株和08Q723菌株制备疫苗时，该疫苗可以有价值地用作预防或治疗牛病毒性腹泻的有效疫苗 因为该疫苗适用于国内基因型，并且与常规疫苗相比具有优异的免疫功能，并且解决了用于牛病毒性腹泻的常规疫苗的问题。
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Patent Agency Ranking
公开(公告)号：KR1020120003416A
公开(公告)日：2012-01-10
申请号：KR1020110136579
申请日：2011-12-16
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 삼양애니팜
CPC classification number: A61K31/4152 , A61K31/4402
Abstract: PURPOSE: A veterinary composition containing sulpyrine and anti-histamine agent is provided to help antibody formation without negative effect. CONSTITUTION: A veterinary composition for reducing animal stress caused by vaccination contains sulpyrine as an active ingredient and is used by mixing with a vaccine. The composition further contains an anti-histamine agent. The anti-histamine agent is chlorpheniramine, diphenhydramine, tripelenamine, promethazine, or veterinarily acceptable salt thereof. The vaccine includes swine fever vaccine or PED-TGE mixed vaccine.
Abstract translation: 目的：提供含有苏吡啶和抗组胺药物的兽用组合物，以帮助抗体形成而没有负面影响。 构成：用于减少由疫苗引起的动物应激的兽用组合物，含有苏拉奇作为活性成分，并与疫苗混合使用。 组合物还含有抗组胺剂。 抗组胺剂是氯苯那敏，苯海拉明，三硒胺，异丙嗪或其兽医学上可接受的盐。 该疫苗包括猪瘟疫苗或PED-TGE混合疫苗。
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37.发明公开소코로나바이러스, 소로타바이러스, 및 소바이러스성설사병바이러스를 동시에 검출할 수 있는 프라이머 및 이를 이용한 소 등 반추류의 설사유발 바이러스 동시 검출법 有权可以同时检测牛病毒弓形虫，牛肝病和杀虫剂的原发性肝炎病毒，以及使用它们的病原体中的病毒类病毒的同时检测方法
公开(公告)号：KR1020100058222A
公开(公告)日：2010-06-03
申请号：KR1020080116959
申请日：2008-11-24
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 인트론바이오테크놀로지
CPC classification number: Y02A50/451 , C12Q1/686 , C12Q1/04 , C12Q2600/16
Abstract: PURPOSE: A bovine corona virus and a method for detecting bovine virus and pestivirus are provided to enable hot-start PCR and suppress non-specific amplification. CONSTITUTION: A primer for detecting bovine diarrheal disease contains a primer for detecting bovine corona virus and a prime for detecting rotavirus. The primer for detecting bovine corona virus is a sequence of amplifying a gene encoding hemagglutinin esterase of bovine corona virus. The primer for detecting rotavirus amplifies a sequence corresponding to VP7 among genes encoding capsid gene of rotavirus. A method for detecting bovine corona virus, bovine rotavirus, and bovine diarrheal disease comprises: a step of extracting RNA, a step of performing PCR using the RNA, and a step of analyzing the PCR product.
Abstract translation: 目的：提供牛冠状病毒和检测牛病毒和瘟病毒的方法，以实现热启动PCR和抑制非特异性扩增。 构成：用于检测牛腹泻病的底漆含有检测牛冠状病毒的引物和用于检测轮状病毒的质粒。 用于检测牛冠状病毒的引物是扩增编码牛冠状病毒血凝素酯酶的基因的序列。 用于检测轮状病毒的引物在轮状病毒的编码衣壳基因的基因中扩增与VP7相对应的序列。 一种检测牛冠状病毒，牛轮状病毒和牛腹泻病的方法包括：提取RNA的步骤，使用RNA进行PCR的步骤，以及分析PCR产物的步骤。
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38.发明授权돼지 ＩｇＧ의 Ｆｃ 도메인의 세포 표면 발현용 벡터, 상기 벡터에 의해 형질전환된 숙주세포, 상기 숙주세포를 이용한 돼지 질병 관련 바이러스 백신 및 이의 제조방법 有权用于猪IgG Fc多糖的细胞表面表达载体，用载体转化的宿主细胞，针对猪疾病相关病毒的疫苗及其使用宿主细胞的制备方法
公开(公告)号：KR100923664B1
公开(公告)日：2009-10-28
申请号：KR1020070096698
申请日：2007-09-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 코미팜
Abstract: 본 발명은 돼지 면역글로불린 지(Immunoglobulin G; 이하 “IgG”라 한다)의 에프씨(Crystallized Fragment; 이하 “Fc”한다) 도메인의 세포 표면 발현용 벡터, 상기 벡터에 의해 형질전환된 숙주세포 및 상기 숙주세포를 이용한 돼지 질병 관련 바이러스에 대한 백신의 제조방법에 관한 것이다. 본 발명에 따른 숙주세포에 바이러스를 증식시키는 경우 바이러스 외피에 Fc가 포함됨으로써 다양한 돼지 질병 관련 바이러스에 대하여 면역능이 우수한 백신을 손쉽게 생산할 수 있도록 해준다.
돼지 IgG, Fc 유전자, 표면 발현, 벡터-
公开(公告)号：KR100449906B1
公开(公告)日：2004-09-22
申请号：KR1020010082977
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
Abstract: PURPOSE: A diagnostic method of foot-and-mouth disease using a recombinant FMCV 2C non-structural protein and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant FMCV 2C non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant vector is prepared by cloning the gene encoding the recombinant FMCV 2C non-structural protein of SEQ ID NO: 1. The recombinant FMCV 2C non-structural protein is expressed by transformation of a cell with the recombinant vector. A hybridoma cell line(KCTC 10137BP) is prepared by introducing the recombinant FMCV 2C non-structural protein into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A recombinant FMCV 2C non-structural protein specific monoclonal antibody is produced from the hybridoma cell line(KCTC 10137BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant FMCV 2C non-structural protein specific monoclonal antibody in a coating buffer solution and pouring the diluate on a plate; (2) washing the plate to remove unattached monoclonal antibodies; (3) reacting the recombinant FMCV 2C non-structural protein with the plate; (4) washing the plate to remove unreacted recombinant FMCV 2C non-structural proteins; (5) reacting the testing serum with the plate; (6) washing the plate to remove unreacted testing serum; (7) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (8) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用重组FMCV 2C非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速和更准确地诊断口蹄疫。 构成：编码来源于韩国口蹄疫病毒O / SKR / 2000的重组FMCV 2C非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。重组载体通过克隆编码 重组FMCV 2C非结构蛋白SEQ ID NO：1。通过用重组载体转化细胞表达重组FMCV 2C非结构蛋白。 通过将重组FMCV 2C非结构蛋白导入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系（KCTC 10137BP）。 从杂交瘤细胞系（KCTC 10137BP）产生重组FMCV 2C非结构蛋白特异性单克隆抗体。 一种口蹄疫诊断方法，包括以下步骤：（1）将重组FMCV 2C非结构蛋白特异性单克隆抗体稀释于包被缓冲液中，并将稀释液倒入平板中; （2）清洗平板以去除未附着的单克隆抗体; （3）使重组FMCV 2C非结构蛋白与平板反应; （4）洗涤平板以除去未反应的重组FMCV 2C非结构蛋白; （5）使测试血清与平板反应; （6）洗涤平板以除去未反应的测试血清; （7）使与试验血清中的口蹄疫病毒抗体结合的缀合物与试验血清具有酶，放射性物质或荧光物质的步骤; 和（8）测量酶反应的强度，与缀合物的荧光反应或辐射反应。
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公开(公告)号：KR1020030052859A
公开(公告)日：2003-06-27
申请号：KR1020010082977
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
CPC classification number: C12N5/163 , C07K16/1009 , C07K2317/14 , C12N2770/32131 , G01N33/56983 , G01N33/577 , G01N2333/09
Abstract: PURPOSE: A diagnostic method of foot-and-mouth disease using a recombinant FMCV 2C non-structural protein and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant FMCV 2C non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant vector is prepared by cloning the gene encoding the recombinant FMCV 2C non-structural protein of SEQ ID NO: 1. The recombinant FMCV 2C non-structural protein is expressed by transformation of a cell with the recombinant vector. A hybridoma cell line(KCTC 10137BP) is prepared by introducing the recombinant FMCV 2C non-structural protein into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A recombinant FMCV 2C non-structural protein specific monoclonal antibody is produced from the hybridoma cell line(KCTC 10137BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant FMCV 2C non-structural protein specific monoclonal antibody in a coating buffer solution and pouring the diluate on a plate; (2) washing the plate to remove unattached monoclonal antibodies; (3) reacting the recombinant FMCV 2C non-structural protein with the plate; (4) washing the plate to remove unreacted recombinant FMCV 2C non-structural proteins; (5) reacting the testing serum with the plate; (6) washing the plate to remove unreacted testing serum; (7) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (8) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用重组FMCV 2C非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速，准确地诊断口蹄疫。 构成：编码来自韩国口蹄疫病毒O / SKR / 2000的重组FMCV 2C非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。通过克隆编码 SEQ ID NO：1的重组FMCV 2C非结构蛋白。通过用重组载体转化细胞来表达重组FMCV 2C非结构蛋白。 通过将重组FMCV 2C非结构蛋白引入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合，来制备杂交瘤细胞系（KCTC 10137BP）。 从杂交瘤细胞系（KCTC 10137BP）产生重组FMCV 2C非结构蛋白特异性单克隆抗体。 口蹄疫的诊断方法包括以下步骤：（1）在包衣缓冲溶液中稀释重组FMCV 2C非结构蛋白特异性单克隆抗体，将稀释物倒入板上; （2）洗涤板以除去未附着的单克隆抗体; （3）使重组FMCV 2C非结构蛋白与板反应; （4）洗涤板以除去未反应的重组FMCV 2C非结构蛋白; （5）使测试血清与板反应; （6）洗板以除去未反应的检测血清; （7）使与测试血清中的口蹄疫病毒抗体结合并具有酶，放射性物质或荧光材料的缀合物与测试血清反应; 和（8）测量酶反应的强度，荧光反应或与缀合物的辐射反应。
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