公开(公告)号：KR101491792B1

公开(公告)日：2015-02-13

申请号：KR1020130108361

申请日：2013-09-10

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 정병열 ,  김애란 ,  정석찬 ,  이지연 ,  박혜연 ,  서병주

IPC: C12N1/20 ,  C12Q1/02 ,  A61K39/02

Abstract: 본 발명은 돼지 흉막폐렴에 대한 예방 효과를 갖는 신규한 액티노바실러스 플로르뉴모니아 혈청형 5번 균주 KACC 91768P, 상기 균주를 포함하는 돼지 흉막폐렴 진단용 조성물 및 상기 균주를 포함하는 돼지 흉막폐렴 예방용 백신 조성물에 관한 것이다.

Abstract translation: 本发明涉及一种具有预防猪胸膜肺炎的效果的新型放线杆菌胸膜血管炎血清型5株KACC 91768P， 用于诊断含有该菌株的猪胸膜肺炎的组合物; 以及用于预防含有该菌株的猪胸膜肺炎的疫苗组合物。

公开(公告)号：KR101437389B1

公开(公告)日：2014-09-04

申请号：KR1020120088442

申请日：2012-08-13

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 허문 ,  김지연 ,  이기찬 ,  강성일 ,  정석찬

IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/04

Abstract: 본 발명은 브루셀라 캐니스 균 특이적 동정용 프라이머 세트 및 이를 이용한 개 브루셀라 균의 검출 방법에 관한 것이다. 본 발명에 따른 프라이머 세트는 개 브루셀라 병을 일으키는 브루셀라 캐니스 균만을 특이적으로 검출하여 개 브루셀라 병을 신속하게 검색 및 진단할 수 있어, 병의 전염경로, 역학적 연구, 진단 및 치료 등에 유용하게 사용할 수 있다.

公开(公告)号：KR1020140081335A

公开(公告)日：2014-07-01

申请号：KR1020120150964

申请日：2012-12-21

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 조윤상 ,  정석찬 ,  오성욱 ,  이종걸

IPC: C12Q1/04 ,  G01N33/50 ,  G01N33/98

CPC classification number: G01N33/0047 ,  G01N2800/26

Abstract: The present invention relates to a method for screening bovine tuberculosis using an electronic nose, and more specifically, to a method for screening bovine tuberculosis using an electronic nose provided with a metal oxide sensor (MOS). According to the present invention, labor and costs are remarkably reduced by automatically screening serum infected with bovine tuberculosis and serum not infected with bovine tuberculosis with the electronic nose through a simple one-time sample injection process, and thereby rapidly screening a large number of cows with suspected bovine tuberculosis infection.

Abstract translation: 本发明涉及使用电子鼻筛选牛结核病的方法，更具体地，涉及使用设置有金属氧化物传感器（MOS）的电子鼻筛选牛结核病的方法。 根据本发明，通过简单的一次性注射过程，通过用电子鼻自动筛选感染牛结核的血清和未被牛结核感染的血清的劳动和成本显着降低，从而快速筛选大量的牛 疑似牛结核感染。

公开(公告)号：KR1020140022195A

公开(公告)日：2014-02-24

申请号：KR1020120088442

申请日：2012-08-13

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 허문 ,  김지연 ,  이기찬 ,  강성일 ,  정석찬

IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/04

CPC classification number: C12Q1/6844 ,  C12Q1/04 ,  C12Q1/689

Abstract: The present invention relates to a primer set for Brucella canis strain-specific identification and a method for detecting Brucella canis strain using the same. A primer set according to the present invention specifically detects Brucella canis strains causing canine brucellosis, thereby promptly searching and diagnosing canine brucellosis and being used in a route of infection of a disease, mechanical research, diagnosis, treatments, etc.

Abstract translation: 本发明涉及布鲁氏杆菌菌株特异性鉴定的引物组以及使用该鉴定的布鲁氏杆菌菌株的检测方法。 根据本发明的引物组特异性检测引起犬布鲁氏杆菌病的布鲁氏菌属菌株，从而及时搜寻和诊断犬布鲁氏菌病并用于疾病感染，机械研究，诊断，治疗等途径。

公开(公告)号：KR1020140005458A

公开(公告)日：2014-01-15

申请号：KR1020120072679

申请日：2012-07-04

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 강승원 ,  유미선 ,  노진형 ,  권창희 ,  정석찬

IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/04

CPC classification number: C12Q1/6844 ,  C12N15/11 ,  C12Q1/04

Abstract: The present invention relates to a primer set for diagnosing Korea type sacbrood virus, and a method for diagnosing Korea type sacbrood virus using the primer set. The primer set for diagnosing Korea type sacbrood virus has an excellent effect of specifically extracting Korea type sacbrood virus from samples of sacbrood virus infection, thereby being used for extracting and diagnosing Korea type sacbrood virus. [Reference numerals] (AA) Lane 1-4 : Sample of honey bee infected with Korea type sacbrood virus; (BB) Lane 5-7 : Sample of honey bee infected with other type sacbrood virus; (CC) Lane 8 : Positive control group; (DD) Lane 9 : Negative control group

Abstract translation: 本发明涉及用于诊断韩国型囊状病毒的引物组，以及使用引物组诊断韩国型囊状病毒的方法。 用于诊断韩国型sacbrood病毒的引物组具有从sacbrood病毒感染样品中特异性提取韩国型sacbrood病毒的优异效果，用于提取和诊断韩国型sacbrood病毒。 （AA）泳道1-4：用韩国型sacbrood病毒感染的蜂蜜样品; （BB）泳道5-7：用其他类型的囊状病毒感染的蜂蜜样品; （CC）泳道8：阳性对照组; （DD）泳道9：阴性对照组

公开(公告)号：KR1020140000810A

公开(公告)日：2014-01-06

申请号：KR1020120068128

申请日：2012-06-25

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김재명 ,  구복경 ,  강환구 ,  김나래 ,  이햇님 ,  이혁미 ,  정석찬

IPC: C12Q1/68 ,  G01N33/569 ,  C12Q1/04

CPC classification number: G01N33/5695 ,  C12Q1/04 ,  C12Q1/686

Abstract: The present invention relates to a detection method using magnetic nanoparticles for rapidly isolating Mycobacterium avium subsp. paratuberculosis from fecal samples and, more specifically, to a method for detecting the Mycobacterium avium subsp. paratuberculosis which isolates the Mycobacterium avium subsp. paratuberculosis with high sensitivity which can be existed in animal fecal samples using the magnetic nanoparticles to which Mycobacterium avium subsp. paratuberculosis specific antibodies are combined and rapidly and simply checks infection of the Mycobacterium avium subsp. paratuberculosis by performing polymerase chain reaction using primers which are specifically combined to DNA of the Mycobacterium avium subsp. paratuberculosis. According to the present invention, the detection method using the magnetic nanoparticles for rapidly isolating the Mycobacterium avium subsp. paratuberculosis from fecal samples can easily detect the Mycobacterium avium subsp. paratuberculosis even if small number of the Mycobacterium avium subsp. paratuberculosis is existed in biological samples such as feces and prevent contagion to different entities by isolating the infected entities from an allied army by performing the method simply and rapidly.

Abstract translation: 本发明涉及使用磁性纳米颗粒快速分离鸟分枝杆菌亚种的检测方法。 来自粪便样品的副结核病，更具体地，涉及用于检测鸟分枝杆菌亚种的方法。 分离鸟分枝杆菌亚种的副结核病 具有高灵敏度的副结核病，其可以存在于动物粪便样品中，使用鸟分枝杆菌亚种的磁性纳米颗粒。 组合结核分枝杆菌特异性抗体并快速简单地检查鸟分枝杆菌感染。 使用与鸟分枝杆菌亚种DNA的特异性组合的引物进行聚合酶链反应，来治疗副结核病。 副结核病。 根据本发明，使用磁性纳米颗粒快速分离鸟分枝杆菌亚种的检测方法。 来自粪便样本的副结核病可以很容易地检测到鸟分枝杆菌。 副结核病即使少数分枝杆菌分枝杆菌。 副结核病存在于粪便等生物样本中，通过简单，快速地进行方法，将受感染的实体与盟军分离，防止对不同实体的传染。

公开(公告)号：KR1020130054771A

公开(公告)日：2013-05-27

申请号：KR1020110120356

申请日：2011-11-17

Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  솔젠트 (주)

Inventor： 구복경 ,  김영일 ,  김혜지 ,  권진욱 ,  정석찬 ,  명현군 ,  공시원

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/04

CPC classification number: Y02A50/451 ,  C12Q1/686 ,  C12Q1/04

Abstract: PURPOSE: A method for detecting a quinolone resistant Campylobacter variant in a biological sample and a primer set and a kit for PCR for the same are provided to cheaply and simply detect and distinguish quinolone resistant variants of C. jejuni and C.coli. CONSTITUTION: A method for distinguishing and detecting a wild type and a quinolone resistant variant type of C. jejuni and C.coli in a biological sample comprises: a step of isolating DNA from the biological sample; a step of performing PCR using the DNA as a template and a primer set for PCR containing an oligonucleotide of sequence numbers 1, 2, 3, 4, 5, and 6; and a step of analyzing a PCR product and distinguishing wild type and variant type.

Abstract translation: 目的：提供一种用于检测生物样品中的喹诺酮抗性弯曲杆菌变异体的方法，以及引物组和用于PCR的PCR试剂盒，以廉价且简单地检测和区分空肠弯曲杆菌和C.coli的喹诺酮抗性变体。 构成：在生物样品中鉴别和检测野生型和喹诺酮抗性变体型空肠弯曲杆菌和C.coli的方法包括：从生物样品中分离DNA的步骤; 使用DNA作为模板进行PCR的步骤和含有序列号1,2,3,4,5和6的寡核苷酸的PCR引物组; 以及分析PCR产物并区分野生型和变异型的步骤。

公开(公告)号：KR101144994B1

公开(公告)日：2012-06-27

申请号：KR1020080041975

申请日：2008-05-06

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 강승원 ,  최세은 ,  이향심 ,  진영화 ,  정석찬

IPC: A61K39/002 ,  A61K39/00

Abstract: PURPOSE: A vaccine of neosporosis and a method for producing neosporosis are provided to induce infection of low level and prevent neosporosis. CONSTITUTION: A vaccine for bovine neosporosis contains Neospora caninum antigen. The vaccine further comprises Polygen^TM, IMS1313^TM, or PBS^TM as an adjuvant. A method for producing the vaccine comprises: a step of culturing Neospora protozoa in a cell; a step of collecting cultured Neospora protozoa; and a step of treating the Neospora protozoa with antigen.

公开(公告)号：KR1020120024290A

公开(公告)日：2012-03-14

申请号：KR1020100087127

申请日：2010-09-06

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 강승원 ,  최세은 ,  권창희 ,  이희수 ,  정갑수 ,  정석찬

IPC: G01N33/544 ,  G01N33/569

Abstract: PURPOSE: A method for binding Leishmania infantum antigen to latex beads is provided to enable simple and cheap diagnosis without expensive equipment. CONSTITUTION: A method for binding Leishmania infantum(ATCC 50134) antigen to latex beads comprises: a step of the Leishmania infantum antigen in 45mM~55mM HEPES(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and 145-155mM NaCl buffer in a concentration of 1.45mg/ml-1.55mg/ml; a step of reacting the latex beads into the diluted antigen at 35-39°C for 0.5-1.5 hours and then reacting at 3-5°C for 8-12 hours; a step of centrifuging the reacted antigen at 5,000rpm-7000rpm for 15-25 minutes; and a step of treating 10% BSA(Bovine Albumin Serum) for 2.5-3.5 hours.

Abstract translation: 目的：提供一种将利什曼原虫婴幼儿抗原结合到乳胶珠上的方法，以便在没有昂贵的设备的情况下进行简单而便宜的诊断。 构成：将利什曼原虫（ATCC 50134）抗原结合到乳胶珠的方法包括：在45mM〜55mM HEPES（4-（2-羟乙基）-1-哌嗪乙磺酸）和145-155mM NaCl缓冲液中的利什曼原虫婴幼儿抗原的步骤 浓度为1.45mg / ml-1.55mg / ml; 将乳胶珠在35-39℃下反应0.5-1.5小时，然后在3-5℃下反应8-12小时; 以5000rpm-7000rpm离心反应的抗原15-25分钟的步骤; 以及将10％BSA（牛白蛋白血清）处理2.5-3.5小时的步骤。

公开(公告)号：KR1020110052295A

公开(公告)日：2011-05-18

申请号：KR1020090109275

申请日：2009-11-12

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 조윤상 ,  오진식 ,  조동희 ,  황인영 ,  허문 ,  정석찬 ,  양창범 ,  장영부 ,  강성일 ,  하건우 ,  오연경 ,  정승교

IPC: G01N33/68 ,  C07K14/35 ,  C12R1/32

Abstract: PURPOSE: A method for diagnosing cattle tuberculosis using intradermal diagnostic composition is provided to easily prepare a large amount of intradermal diagnostic composition and to ensure diagnosis accuracy. CONSTITUTION: An intradermal diagnostic composition for testing cattle tuberculosis contains recombinant proteins of Esat6, HspX, PhoS, MPB70, MPB64, MPB83, Ag85, and SahH. A method for diagnosing tuberculosis of mammal except for human is performed using the intradermal diagnostic composition containing the recombinant proteins. The recombinant protein is prepared by isolating from Mycobacterium bovis and performing PCR.

Abstract translation: 目的：提供使用皮内诊断组合物诊断牛结核病的方法，以容易地制备大量皮内诊断组合物并确保诊断准确性。 构成：用于测试牛结核的皮内诊断组合物含有Esat6，HspX，PhoS，MPB70，MPB64，MPB83，Ag85和SahH的重组蛋白。 使用含有重组蛋白质的皮内诊断组合物进行用于诊断除人以外的哺乳动物结核病的方法。 通过从牛分枝杆菌中分离并进行PCR来制备重组蛋白。