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公开(公告)号：KR1020030052859A
公开(公告)日：2003-06-27
申请号：KR1020010082977
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
CPC classification number: C12N5/163 , C07K16/1009 , C07K2317/14 , C12N2770/32131 , G01N33/56983 , G01N33/577 , G01N2333/09
Abstract: PURPOSE: A diagnostic method of foot-and-mouth disease using a recombinant FMCV 2C non-structural protein and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant FMCV 2C non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant vector is prepared by cloning the gene encoding the recombinant FMCV 2C non-structural protein of SEQ ID NO: 1. The recombinant FMCV 2C non-structural protein is expressed by transformation of a cell with the recombinant vector. A hybridoma cell line(KCTC 10137BP) is prepared by introducing the recombinant FMCV 2C non-structural protein into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A recombinant FMCV 2C non-structural protein specific monoclonal antibody is produced from the hybridoma cell line(KCTC 10137BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant FMCV 2C non-structural protein specific monoclonal antibody in a coating buffer solution and pouring the diluate on a plate; (2) washing the plate to remove unattached monoclonal antibodies; (3) reacting the recombinant FMCV 2C non-structural protein with the plate; (4) washing the plate to remove unreacted recombinant FMCV 2C non-structural proteins; (5) reacting the testing serum with the plate; (6) washing the plate to remove unreacted testing serum; (7) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (8) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用重组FMCV 2C非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速，准确地诊断口蹄疫。 构成：编码来自韩国口蹄疫病毒O / SKR / 2000的重组FMCV 2C非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。通过克隆编码 SEQ ID NO：1的重组FMCV 2C非结构蛋白。通过用重组载体转化细胞来表达重组FMCV 2C非结构蛋白。 通过将重组FMCV 2C非结构蛋白引入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合，来制备杂交瘤细胞系（KCTC 10137BP）。 从杂交瘤细胞系（KCTC 10137BP）产生重组FMCV 2C非结构蛋白特异性单克隆抗体。 口蹄疫的诊断方法包括以下步骤：（1）在包衣缓冲溶液中稀释重组FMCV 2C非结构蛋白特异性单克隆抗体，将稀释物倒入板上; （2）洗涤板以除去未附着的单克隆抗体; （3）使重组FMCV 2C非结构蛋白与板反应; （4）洗涤板以除去未反应的重组FMCV 2C非结构蛋白; （5）使测试血清与板反应; （6）洗板以除去未反应的检测血清; （7）使与测试血清中的口蹄疫病毒抗体结合并具有酶，放射性物质或荧光材料的缀合物与测试血清反应; 和（8）测量酶反应的强度，荧光反应或与缀合物的辐射反应。
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公开(公告)号：KR100261050B1
公开(公告)日：2000-07-01
申请号：KR1019980000443
申请日：1998-01-10
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: G01N33/53
Abstract: PURPOSE: Provided is a device for detecting antibody of Aujeszky's disease. And a method for diagnosing Aujeszky virus more rapidly and correctly using the same is also provided. CONSTITUTION: The device for detecting antibody of Aujeszky's disease is composed of; i) a nitrocellulose membrane (5), absorption pad (3), supporting plate (6), and soaking section (4). The method for detecting antibodies of Aujeszky virus is comprised of the following steps of: i) spreading antigens (1) of Aujeszky virus and anti-swine IgG (2) evenly on the nitrocellulose membrane (5); and ii) reacting the antigens (1) and IgG (2) with antibodies of Aujeszky virus in the soaking section (4), which shows different colors depending on the existence of antibodies.
Abstract translation: 目的：提供了一种用于检测Aujeszky病的抗体的装置。 还提供了使用该方法更快速和正确地诊断Aujeszky病毒的方法。 构成：检测Aujeszky病抗体的装置由以下组成： i）硝酸纤维素膜（5），吸收垫（3），支撑板（6）和浸泡部分（4）。 用于检测Aujeszky病毒抗体的方法包括以下步骤：i）将Aujeszky病毒和抗猪IgG（2）的抗原（1）均匀地铺展在硝酸纤维素膜（5）上; 和ii）在浸泡部分（4）中使抗原（1）和IgG（2）与Aujeszky病毒的抗体反应，其根据抗体的存在显示不同的颜色。
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公开(公告)号：KR1020000019418A
公开(公告)日：2000-04-06
申请号：KR1019980037509
申请日：1998-09-11
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: Recombinant porcine Aujesky's disease virus provides safe vector vaccine against porcine diseases and can be used to produce effective vaccine against other diseases if antigen of other virus is introduced in it. CONSTITUTION: Recombinant porcine Aujesky's disease virus is useful to prepared a vaccine vector. The vector is constructed by; deletion of K gene; insertion of IL-2 gene; deletion of gI gene; insertion of beta-galactosidase gene as a maker gene; and insertion of foreign gene coding antigen for virus pathogen. Thereby, immunity is improved.
Abstract translation: 目的：重组猪Aujesky病病毒提供安全的针对猪疾病的载体疫苗，如果其中引入其他病毒的抗原，可用于产生针对其他疾病的有效疫苗。 构成：重组猪Aujesky病病毒可用于制备疫苗载体。 矢量由...构建; 缺失K基因; 插入IL-2基因; 缺失gI基因; 插入β-半乳糖苷酶基因作为制造者基因; 并插入外源基因编码抗原用于病毒病原体。 从而，免疫力得到改善。
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44.发明公开사람 아데노바이러스 2형으로부터 제작한 재조합 발현벡터 및 그를 이용한 돼지 오제스키병 바이러스 GP50 유전자 재조합 사람아데노바이러스를 선발하는 방법 有权人类腺病毒2型重组荧光显微镜和GP50基因重组人胰腺炎患者的选择方法奥曲斯病毒病毒
公开(公告)号：KR1020000003768A
公开(公告)日：2000-01-25
申请号：KR1019980025040
申请日：1998-06-29
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: C12N15/861 , C07K14/005
Abstract: PURPOSE: Provided are a fluorescent vector, which is used for the production of vaccine of porcine Aujesky's disease and a method for selecting recombination virus using the same. CONSTITUTION: A fluorescent vector pHAVE3 is prepared by amplifying pVIII gene corresponding to 5' region of E3 gene of human Adenovirus type II, a partial sequence(27100-28811bp) having 1,712base, before open reading frame of E3 gene being 19kd, a partial sequence(30,851-32,760bp) having 1,910base, after E3-2 polyadenylation signal corresponding to 3' region of E3 gene; cloning them into SpeI of plasmid pGEMT; and inserting BamH I restriction site
Abstract translation: 目的：提供一种荧光载体，其用于生产猪Aujesky病疫苗，以及使用该载体选择重组病毒的方法。 构成：荧光载体pHAVE3通过扩增对应于人腺病毒II型E3基因的5'区域的pVIII基因，其具有1,712碱基的部分序列（27100-28811bp），E3基因的开放阅读框为19kd，部分 序列（30,851-32760bp）具有1,910个碱基，E3-2多聚腺苷酸化信号对应于E3基因的3'区域; 将其克隆入SpeI质粒pGEMT; 并插入BamH I限制位点
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公开(公告)号：KR102245767B1
公开(公告)日：2021-04-28
申请号：KR1020190071063
申请日：2019-06-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 바이오노트
Abstract: 인터류킨-7, 인터류킨-12 또는이들의조합을포함하는소 결핵병을진단하기위한감마인터페론법(IFN-γ assay)에적용되는혈액보존용조성물및 이를이용한혈액보존방법에관한것이다.
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Patent Agency Ranking
公开(公告)号：KR102168646B1
公开(公告)日：2020-10-21
申请号：KR1020200026733
申请日：2020-03-03
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 고려비엔피
IPC: C07K14/005 , A61K39/12 , A61P31/20
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公开(公告)号：KR1020160010818A
公开(公告)日：2016-01-28
申请号：KR1020140091163
申请日：2014-07-18
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본발명은양성감별마커를포함한돼지열병예방용백신조성물에관한것으로, 보다상세하게는돼지열병예방용백신에있어야외감염과백신에의한항체형성을감별할수 있는양성감별마커를포함한돼지열병예방용백신조성물에관한것이다. 선별된양성마커에대해서는야외감염과백신에의한항체형성을감별하기위해경쟁적방법을사용하여분석방법을확립하였고, 선별된양성마커의면역원성을동물실험을통해입증하여양성마커를포함한돼지열병예방용백신조성물이야외감염과백신에의한감별에용이하게사용될수 있다. 나아가상기마커를포함한돼지열병바이러스의사용은가축전염병의예방및 치료에효율적으로기여할수 있다.
Abstract translation: 本发明涉及一种用于预防猪瘟的疫苗组合物，包括阳性检查标记，更具体地，涉及用于预防猪瘟的疫苗组合物，包括可以区分室外感染和由疫苗形成抗体的阳性检查标记。 所选择的阳性标记物具有使用竞争性方法的分析方法，以便通过室外感染和疫苗鉴别所选择的阳性标记物的抗体形成，并且用于预防猪瘟的疫苗组合物（包括阳性检查标记物）可以容易地 用于通过室外感染和疫苗区分抗体形成，因为通过动物实验检查所选阳性标记物的免疫原性。 此外，使用包括标记在内的猪瘟病毒可以有效地促进牛的感染性疾病的预防和治疗。
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48.发明公开재조합 광견병 바이러스, 이를 포함하는 광견병 예방용 백신 조성물 및 야외 광견병 바이러스와의 감별을 위한 멀티플렉스 RT-PCR 无效用于预防RABIES的重组病毒病毒和含有其的疫苗组合物，以及用于分化病毒性病毒的多重RT-PCR
公开(公告)号：KR1020150138956A
公开(公告)日：2015-12-11
申请号：KR1020140066041
申请日：2014-05-30
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: C12N7/00
Abstract: 본발명은재조합광견병바이러스, 이를포함하는광견병예방용백신조성물및 야외광견병바이러스와의감별을위한멀티플렉스 RT-PCR에관한것으로, 구체적으로광견병바이러스의 G 단백질에서 333번째아미노산이글루탐산으로치환되고 194번째아미노산이세린으로치환되어안정성과효과가우수해진재조합광견병바이러스, 이를포함하는광견병예방용백신조성물및 이재조합광견병바이러스와야외광견병바이러스를명확하게감별할수 있도록하는멀티플렉스 RT-PCR에관한것이다. 본발명의재조합광견병바이러스는 G 단백질의 333번째아미노산및 194번째아미노산이각각글루탐산과세린으로치환되어기존생백신주에비해매우안전하고, 경구투여용으로사용할수 있어기존광견병백신의문제점을해결할수 있다. 또한, 본발명의재조합광견병바이러스제조방법에따르면상기와같은우수한재조합광견병바이러스를숙주세포내에서효율적으로생산할수 있어, 대량생산을통해보다많이백신을보급함으로써광견병의예방에크게기여할수 있을것이라기대된다. 그리고본 발명의재조합광견병바이러스검출방법또는재조합광견병바이러스검출용키트를사용하면본 발명의재조합광견병바이러스와야외바이러스의감염여부를명확하게구별하여확인할수 있으므로, 백신이야생동물에게투여가되었는지혹은광견병바이러스에감염이되어광견병을유발할수 있는지의여부를용이하게판단할수 있다.
Abstract translation: 本发明涉及重组狂犬病毒，包含其的重组狂犬病病毒疫苗组合物和用于检测重组狂犬病病毒的多重RT-PCR。 更具体地说，通过用谷氨酸代替狂犬病病毒的G蛋白中的第333位氨基酸，用丝氨酸替代第194位氨基酸来制备重组狂犬病病毒，以增强其效果和稳定性。 多重RT-PCR可用于准确识别室外狂犬病病毒。 重组狂犬病病毒是高度安全的，可用于口服给药以解决常规狂犬病疫苗的问题。 根据本发明，可以使用制备重组狂犬病病毒的方法，在宿主细胞中有效地产生优良的重组狂犬病病毒，通过批量生产分发疫苗，并有效预防疫苗。 根据本发明，可以使用重组狂犬病病毒检测方法或重组狂犬病病毒检测试剂盒来准确鉴定和区分重组狂犬病病毒的感染和重组狂犬病病毒的室外感染，以便容易地确定是否 疫苗被施用于野生动物，或者野生动物是否被感染并且能够诱导狂犬病感染。
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公开(公告)号：KR1020130055038A
公开(公告)日：2013-05-28
申请号：KR1020110118179
申请日：2011-11-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/866 , C12N7/01 , C07K16/08
Abstract: PURPOSE: A recombinant antibody for diagnosing porcine epidemic diarrhea virus is provided to easily maintain or store an antibody producing strain. CONSTITUTION: A method for preparing a recombinant antibody for diagnosing porcine epidemic diarrhea virus comprises: a step of preparing a recombinant baculovirus vector containing a DNA fragment of sequence number 1; a step of transfecting baculovirus full length DNA Bac-N-Blue and the recombinant baculovirus vector into an insect cell and preparing recombinant baculovirus; and a step of culturing the recombinant baculovirus in the insect cell. The vector is pBlueBac4.5His. The insect cell is sf-9 cell.
Abstract translation: 目的：提供用于诊断猪流行性腹泻病毒的重组抗体，以容易地维持或储存产生抗体的菌株。 构成：制备用于诊断猪流行性腹泻病毒的重组抗体的方法，包括：制备含有序列号1的DNA片段的重组杆状病毒载体的步骤; 将杆状病毒全长DNA Bac-N-Blue和重组杆状病毒载体转染入昆虫细胞并制备重组杆状病毒的步骤; 以及在昆虫细胞中培养重组杆状病毒的步骤。 载体是pBlueBac4.5His。 昆虫细胞是sf-9细胞。
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50.发明授权유전자 마커 및 혈청학적 마커를 이용하여 백신바이러스와 야외감염 바이러스를 감별할 수 있도록 재조합한 특정 키메릭 페스티바이러스를 포함하는 돼지열병 예방용 생백신 有权含有KD26_E2LOM的经典猪瘟减毒疫苗，通过使用血清学标记和基因标记来区分疫苗病毒与感染病毒
公开(公告)号：KR101174449B1
公开(公告)日：2012-08-22
申请号：KR1020100010346
申请日：2010-02-04
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본발명은재조합된키메릭페스티바이러스를포함하는돼지열병예방용생백신에관한것으로서, 보다상세하게는돼지열병예방용백신에있어유전자마커및 혈청학적마커를이용하여백신바이러스와야외감염바이러스를감별할수 있도록재조합한키메릭페스티바이러스 KD26_E2LOM을포함하는돼지열병예방용생백신에관한것이다. 현재국내에서사용하고있는돼지열병생백신으로사용되고있는 LOM 바이러스는 PCR을통해증폭한후 제한효소을이용하여야외감염바이러스와구분이가능하나, 혈청학적감별은불가능하다는점에서집단혈청학적스크린이곤란하다는문제점이있었다. 이에본 발명인유전자재조합돼지열병생백신용 KD26_E2LOM 바이러스는혈청학적마커및 유전자마커를가지고있어돼지열병예방용백신으로사용될경우, 야외바이러스와감별이가능하다. 나아가상기돼지열병생백신용바이러스의변이는쉽게추적될수 있다는점에서백신의안전관리또한향상시킬수 있다는장점이있다.
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