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    45.
    发明专利
    未知

    公开(公告)号：NO20024169D0

    公开(公告)日：2002-09-02

    申请号：NO20024169

    申请日：2002-09-02

    Applicant: BASF AG

    Inventor： RESS-LOESCHKE MARION ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

    IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  C12N

    Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

    46.
    发明专利
    未知

    公开(公告)号：NO20021906L

    公开(公告)日：2002-04-26

    申请号：NO20021906

    申请日：2002-04-23

    Applicant: BASF AG

    Inventor： SCHMID ANDREAS ,  WITHOLD BERNARD ,  HAUER BERNHARD ,  BUEHLER BRUNO

    IPC: C12N15/09 ,  C12N1/21 ,  C12P7/24 ,  C12P7/40 ,  C12R1/19 ,  C12R1/38

    Abstract: Production of aromatic aldehyde or carboxylic acid (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate (II). Production of aromatic aldehyde or carboxylic acid of formula (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate of formula (II). [Image] Ar : monocyclic aromatic ring, optionally with one or more substituents; R 1>formyl or carboxy; n : 0-15; R 2>vinyl or (CH 2) m-R 3>, also where R 1>= carboxy, R 2>may be (CH 2) n-CHO; m : n+1; and R 3>hydrogen or hydroxy. Independent claims are also included for the following: (a) recombinant microorganism harboring an expression vector that contains, under control of the alk-regulatory system of Pseudomonas oleovoransGpol, the XMO-encoding genes xylM and xylA, or the AMO-encoding genes alkB, alkG and alkT; and (b) expression construct containing the genes specified in (a), operably linked to the alk-regulatory system.

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