公开(公告)号：CA2633476C

公开(公告)日：2015-04-21

申请号：CA2633476

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： HANZEL DAVID ,  KORLACH JONAS ,  PELUSO PAUL ,  OTTO GEOFFREY ,  PHAM THANG ,  RANK DAVID ,  TURNER STEPHEN

IPC: C12N9/12 ,  C12N9/22 ,  C12N11/00 ,  C12P19/34 ,  C12Q1/68

Abstract: Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.

公开(公告)号：CA2579150C

公开(公告)日：2014-11-25

申请号：CA2579150

申请日：2005-09-16

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： TURNER STEPHEN W ,  KORLACH JONAS

IPC: C12Q1/68 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y20/00 ,  C12M1/40 ,  C40B60/00 ,  G01N21/00 ,  G01N21/75 ,  G02B6/12

Abstract: The present invention relates to optical confinements, method of preparing and methods of using them for analyzing molecules and/or monitoring chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost single-molecular analysis.

公开(公告)号：AU2013240166A1

公开(公告)日：2014-10-30

申请号：AU2013240166

申请日：2013-03-18

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： KORLACH JONAS ,  TURNER STEPHEN ,  CLARK TYSON A

IPC: C12Q1/68 ,  G01N33/48

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, a basic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：CA2867489A1

公开(公告)日：2013-10-03

申请号：CA2867489

申请日：2013-03-18

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： KORLACH JONAS ,  TURNER STEPHEN ,  CLARK TYSON A

IPC: C12Q1/68 ,  G01N33/48

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, a basic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：AU2012216605A1

公开(公告)日：2012-09-20

申请号：AU2012216605

申请日：2012-09-03

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： RANK DAVID R ,  WEGENER JEFFERY ,  KORLACH JONAS ,  ROITMAN DANIEL ,  XU YUE ,  LYLE JOHN ,  TURNER STEPHEN ,  PELUSO PAUL ,  OTTO GEOFF

IPC: C12N11/00 ,  C40B40/04

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

公开(公告)号：DK2002019T3

公开(公告)日：2012-06-18

申请号：DK07754529

申请日：2007-03-29

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： RANK DAVID R ,  WEGENER JEFFERY ,  ROITMAN DANIEL ,  XU YUE ,  LYLE JOHN ,  TURNER STEPHEN ,  KORLACH JONAS ,  PELUSO PAUL ,  OTTO GEOFF ,  CICERO RONALD L

IPC: C12N11/00 ,  C40B40/04

公开(公告)号：CA3063032A1

公开(公告)日：2010-03-25

申请号：CA3063032

申请日：2009-09-15

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZHONG CHENG FRANK ,  LUNDQUIST PAUL ,  FOQUET MATHIEU ,  KORLACH JONAS ,  BAYANDORIAN HOVIG

Abstract: The present invention provides an integrated optical device (2300), comprising:a) a first component (2302) comprising a waveguide (2312) disposed upon or within a substrate and a plurality of nanometer-scale apertures (2308) comprising analyte regions (2310), wherein the analyte regions (2310) are disposed sufficiently proximal to a core of the waveguide (2312) to be illuminated by an evanescent field emanating from the core when optical energy is passed through the waveguide;b) a second component (2304) comprising a microlens array that collects optical energy signals from the analyte regions (2310) and directs the optical energy signals so collected to a detector (2320); andc) a third component (2306) comprising the detector (2320).

公开(公告)号：CA2720247A1

公开(公告)日：2009-12-03

申请号：CA2720247

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： EID JOHN ,  TURNER STEPHEN ,  DALAL RAVI ,  FLUSBERG BENJAMIN ,  KORLACH JONAS ,  LIN STEVEN ,  FEHR ADRIAN ,  CHRISTIANS FRED ,  EMIG ROBIN

IPC: G01N33/48 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/52

Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided.

公开(公告)号：AU2009251881A1

公开(公告)日：2009-12-03

申请号：AU2009251881

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： CHRISTIANS FRED ,  KORLACH JONAS ,  DALAL RAVI ,  EID JOHN ,  LIN STEVEN ,  FEHR ADRIAN ,  EMIG ROBIN ,  FLUSBERG BENJAMIN ,  TURNER STEPHEN

IPC: G01N33/48 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/52

公开(公告)号：AU2008331824A1

公开(公告)日：2009-06-11

申请号：AU2008331824

申请日：2008-12-03

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： OTTO GEOFF ,  EID JOHN ,  KORLACH JONAS ,  HARDENBOL PAUL ,  FLUSBERG BENJAMIN ,  ROITMAN DANIEL

IPC: G01N33/53 ,  G01N33/535 ,  G01N33/573

Abstract: Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.