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    구제역 7가지 혈청형 감별을 위한 유전자 진단키트
    63.
    发明公开
    구제역 7가지 혈청형 감별을 위한 유전자 진단키트 有权
    用于分化病毒和病毒病毒七代的RT-PCR

    公开(公告)号：KR1020110038380A

    公开(公告)日：2011-04-14

    申请号：KR1020090095646

    申请日：2009-10-08

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 김수미 ,  이광녕 ,  박종현 ,  고영준 ,  이향심 ,  권창희

    IPC: C12Q1/68 ,  C12N15/11

    CPC classification number: H04W8/20 ,  C12Q1/6844 ,  C12N15/11

    Abstract: PURPOSE: A method for diagnosing gene for distinguishing seven kinds of serotype of foot-and-mouth disease is provided to ensure fastness and convenience. CONSTITUTION: A method for diagnosing gene for distinguishing seven kinds of serotype of foot-and-mouth comprises a step of diagnosing seven type of serotype(O, A, Asia 1, C, SAT 1, SAT 2, and SAT 3) by different band size. The diagnosis method comprises: a step of diagnosing type O and Asia 1, type A and C, and type SAT 1, SAT 2, and SAT 3. SAT 1, SAT 2 and SAT 3 has common band size. A primer for amplifying type O has a base sequence of sequence numbers 1, 2, 3, and 5.

    Abstract translation: 目的：提供一种诊断口蹄疫七种血清型鉴定基因的方法，以确保牢固性和便利性。 构成：用于区分七种口蹄血清型的基因的诊断方法包括通过不同的方式诊断七种血清型（O，A，亚洲1，C，SAT 1，SAT 2和SAT 3）的步骤 带尺寸。 诊断方法包括：诊断类型O和亚洲1，类型A和C，以及类型SAT 1，SAT 2和SAT 3的步骤.SAT 1，SAT 2和SAT 3具有共同的带尺寸。 用于扩增型O的引物具有序列号1,2,3和5的碱基序列。

    구제역바이러스 아시아 １형 항체를 검출하기 위한 진단방법
    64.
    发明公开
    구제역바이러스 아시아 １형 항체를 검출하기 위한 진단방법 有权
    用于检测禽流感病毒类型的病毒的诊断方法1

    公开(公告)号：KR1020110031012A

    公开(公告)日：2011-03-24

    申请号：KR1020090088714

    申请日：2009-09-18

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 메디안디노스틱

    Inventor： 고영준 ,  이향심 ,  정혜영 ,  허은정 ,  박종현 ,  이광녕 ,  김수미 ,  권창희 ,  주후돈 ,  장병식

    IPC: G01N33/68 ,  C12N15/09 ,  G01N33/563

    Abstract: PURPOSE: A diagnosis method for detecting food-and-mouth disease virus type asia 1 antibody is provided to quickly measure vaccine antibody titer. CONSTITUTION: An antibody for food-and-mouth disease virus type asia 1 is detected using a gene recombinant protein antigen of food-and-mouth disease virus type asia 1 and food-and-mouth disease virus type asia 1-specific monoclonal antibody. A diagnosis method for detecting food-and-mouth disease virus type asia 1 antibody comprises: a step of reacting serotype common antibody 70-17 of food-and-mouth disease virus type asia 1, type C, O, and A in 96-well microplate; a step of washing monoclonal antibodies which are not conjugated on the plate to remove; a step of reacting with a test serum sample; a step of washing test serum sample which is not conjugated; a step of reacting horseradish peroxidase-conjugated type asia 1-specific monoclonal antibody; a step washing monoclonal antibodies which is not conjugated to the plate; and a step of measuring absorbance.

    Abstract translation: 目的：提供一种用于检测食源性病毒亚型1抗体的诊断方法，以快速测定疫苗抗体效价。 构成：使用食品口蹄疫病毒亚型1和口蹄疫病毒型亚洲1型单克隆抗体的基因重组蛋白抗原检测食品口蹄疫病毒亚型1的抗体。 一种用于检测食源病病毒亚型1抗体的诊断方法包括：将食物口蹄疫病毒亚型1（C，O和A型）的血清型共同抗体70-17与96- 微孔板 洗涤未在板上结合以除去的单克隆抗体的步骤; 与测试血清样品反应的步骤; 洗涤不结合的血清样品的步骤; 辣根过氧化物酶偶联型亚洲1型单克隆抗体的反应步骤; 步骤洗涤未与板结合的单克隆抗体; 和测量吸光度的步骤。

    아까바네, 츄잔, 아이노바이러스 균주 및 이를 이용한 혼합바이러스 백신의 제조방법
    67.
    发明公开
    아까바네, 츄잔, 아이노바이러스 균주 및 이를 이용한 혼합바이러스 백신의 제조방법 有权
    AKABANE VIRUS，CHUZAN VIRUS和AINO VIRUS的分离菌株，以及通过混合其制备病毒疫苗的方法

    公开(公告)号：KR1020080036353A

    公开(公告)日：2008-04-28

    申请号：KR1020060102854

    申请日：2006-10-23

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 김연희 ,  탁동섭 ,  임성인 ,  현방훈 ,  김인중 ,  송재영 ,  양동군 ,  권창희 ,  권준헌

    IPC: C12N7/01 ,  A61K39/12

    Abstract: A viral vaccine containing isolated virus strains is provided to improve use safety and immunogenicity by using inactivated viruses, and increase production yield of cows, so that the vaccine is useful for prevention of abortion or stillbirth in cows caused by viruses via mediation of Culicoides spp. A viral vaccine for prevention of abortion or stillbirth in cows contains cultured medium of inactivated virus strains of akabane virus(K-9)(KCTC 18117P), chuzan virus(KSA9910)(KCTC 18118P) and aino virus(YoungAM)(KCTC 18119P), and is produced by: inactivating akabane virus(K-9)(KCTC 18117P), chuzan virus(KSA9910)(KCTC 18118P) and aino virus(YoungAM)(KCTC 18119P) by adding 0.1M BEI(binary ethylene imine) prepared by solubilizing 2-BEA(2-bromoethylamine) in 0.2N NaOH, and treating the solution in a constant temperature water bath of 37 deg. C for 1 hour to them; mixing the 3 kinds of viruses in a weight ratio of 1:1:1; and adding 40-60 wt.% of IMS 1314 as an immunomodulator into the viral mixture.

    Abstract translation: 提供含有分离的病毒株的病毒疫苗，以通过使用灭活病毒提高使用安全性和免疫原性，并提高奶牛的产量，使得疫苗可用于通过Culicoides spp的调解来预防由病毒引起的奶牛的流产或死胎。 用于预防奶牛流产或死胎的病毒疫苗含有阿卡巴因病毒（K-9）（KCTC18117P），Chuzan病毒（KSA9910）（KCTC18118P）和Aino病毒（YoungCAM）（KCTC18119P）的灭活病毒株的培养基， 通过添加由0.1M BEI（二元乙烯亚胺）制备的阿卡巴因病毒（K-9）（KCTC 18117P），Chuzan病毒（KSA9910）（KCTC 18118P）和Aino病毒（YoungAM）（KCTC 18119P） 溶解在0.2N NaOH中的2-BEA（2-溴乙胺），并在37℃恒温水浴中处理该溶液。 C给他们1小时; 混合3种病毒，重量比为1：1：1; 并将40-60重量％的IMS 1314作为免疫调节剂加入到病毒混合物中。

    소 백혈병 바이러스의 유전자 재조합 곤충세포 발현 단백질및 이를 이용한 소 백혈병 진단 방법
    68.
    发明公开
    소 백혈병 바이러스의 유전자 재조합 곤충세포 발현 단백질및 이를 이용한 소 백혈병 진단 방법 有权
    在肠屏障中表达的ENZOOTIC BOVINE LEUKEMIA病毒的重组蛋白和诊断方法

    公开(公告)号：KR1020080010771A

    公开(公告)日：2008-01-31

    申请号：KR1020060071258

    申请日：2006-07-28

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 권창희 ,  양동군 ,  임성인 ,  탁동섭 ,  권준헌

    IPC: C12N15/12

    Abstract: Recombinant proteins of enzootic bovine leukemia virus are provided to improve specificity and sensitivity of detection as compared with conventional antigens by expressing the envelope glycoproteins in baculovirus and secreting the envelope glycoproteins to insect cells. A method for producing a recombinant protein of enzootic bovine leukemia virus expressed in an insect cell comprises the steps of: (1) cloning an envelop glycoprotein of enzootic bovine leukemia virus from the blood of enzootic bovine leukemia positive cows to baculovirus and sequencing the cloned protein; (2) transforming an insect cell with a recombinant virus gene of SEQ ID NO:1 to express a recombinant protein of SEQ ID NO:2; and (3) purifying and concentrating the recombinant protein produced from the insect cell, wherein the recombinant virus gene of SEQ ID NO:1 is prepared by recombination of envelop glycoprotein genes including a whole gp51 gene and a part of gp30 gene.

    Abstract translation: 通过在杆状病毒中表达包膜糖蛋白并将包膜糖蛋白分泌到昆虫细胞中，提供了特异性和灵敏度的检测与常规抗原的特异性和灵敏度。 用于生产在昆虫细胞中表达的特异性牛白血病病毒重组蛋白质的方法包括以下步骤：（1）将来自地方性牛白血病阳性牛的血液中的特异性牛白血病病毒的包膜糖蛋白克隆至杆状病毒，并测序克隆的蛋白质 ; （2）用SEQ ID NO：1的重组病毒基因转化昆虫细胞以表达SEQ ID NO：2的重组蛋白; （3）从昆虫细胞产生的重组蛋白质的纯化和浓缩，其中通过包括整个gp51基因和一部分gp30基因的包膜糖蛋白基因的重组制备SEQ ID NO：1的重组病毒基因。

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