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    기체 생성과 생성된 기체의 함유가 가능한 다공성 고분자 마이크로 또는 나노 입자, 이의 제조 방법 및 용도
    65.
    发明公开
    기체 생성과 생성된 기체의 함유가 가능한 다공성 고분자 마이크로 또는 나노 입자, 이의 제조 방법 및 용도 有权
    产生和承载气体的微量/纳米颗粒及其制备方法及其应用

    公开(公告)号：KR1020110043344A

    公开(公告)日：2011-04-27

    申请号：KR1020090100417

    申请日：2009-10-21

    Applicant: 한국과학기술연구원

    Inventor： 강은아 ,  권익찬 ,  김광명 ,  민현수 ,  최귀원 ,  한문희 ,  이재영

    IPC: C08G61/12 ,  C08J9/16 ,  A61K49/00

    Abstract: PURPOSE: Porous micro/nanoparticles are provided to generate carbon dioxide gas in hydrolysis and to enable use as a contrast agent by holding carbon dioxide even if gas is not added in contrast. CONSTITUTION: Porous micro/nanoparticles are represented by chemical formula 1. In chemical formula 1, a, b, c, and d are 0-1; a+b+c+d = 1; n is an integer of 1-1000; A is a repeating unit represented by chemical formula 2; B is a repeating unit represented by chemical formula 3; C is a repeating unit represented by chemical formula 4; and D is a repeating unit represented by chemical formula 5. In chemical formulae 2-5, R and R' are C1-10 alkyl group or cholesteryl group; e, f, h, i, j, m, o, p, r, s, t, w and x are 0 or 1; g, k, l, q, u, v, y and z are an integer of 1-10; and X is -CH(CH3)- or -CH2-.

    Abstract translation: 目的：提供多孔微/纳米颗粒水解产生二氧化碳气体，即使不添加气体，也可通过保持二氧化碳作为造影剂。 构成：多孔微纳米颗粒由化学式1表示。在化学式1中，a，b，c和d为0-1; a + b + c + d = 1; n为1-1000的整数; A是由化学式2表示的重复单元; B是由化学式3表示的重复单元; C是由化学式4表示的重复单元; D是由化学式5表示的重复单元。在化学式2-5中，R和R'为C 1-10烷基或胆固醇基; e，f，h，i，j，m，o，p，r，s，t，w和x分别为0或1; g，k，l，q，u，v，y和z为1-10的整数; 并且X是-CH（CH 3） - 或-CH 2 - 。

    HBV의 S표면항원에 결합하는 생쥐 항체의 가변영역을 암호화하는 cDNA 및 이를 포함하는 재조합 플라스미드
    69.
    发明授权
    HBV의 S표면항원에 결합하는 생쥐 항체의 가변영역을 암호화하는 cDNA 및 이를 포함하는 재조합 플라스미드 失效
    用于HBV抗体的重组DNA

    公开(公告)号：KR1019960001817B1

    公开(公告)日：1996-02-05

    申请号：KR1019920011992

    申请日：1992-07-06

    Applicant: 한국과학기술연구원

    Inventor： 한문희 ,  홍효정 ,  류춘제 ,  진병래 ,  정홍근

    IPC: C12N15/13

    Abstract: The cloning method for chimeric antibody to S surface antigen of Hepatitis B virus subtype (I) consists of (1) determining N-terminal amino acid sequences of purified murine antibody to (I) with H67 cell line, (2) PCR reacting cDNA from H67 with primers for heavy chain as 5'-CCC GAA TTC GAG GT(TC) CAG CTG CA(GA) CA(GA) TCT GG(GA) TCT GG(GA) CCT G-3' and 5-CGG TCG ACA GGG ATC CAG AGT TCC AGG GTC AC-3' to get plasmid pMHV-S (KCTC 0044BP), (3) same as (2) for light chain as 5'-CCC GAA TTC GAC ATT GTG CTG AC(CA) CA(GA) TCT CC(TA) GCT-3' and 5'-CCC GCC TTG GAC ATT GTG CTG AC(CA) CA(GA) TCT CC(TA) GCT-3' to get plasmid pMKV-S (KCTC 0045BP), and (4) sequencing the genes cloned.

    Abstract translation: 乙型肝炎病毒亚型（I）的S表面抗原嵌合抗体的克隆方法包括：（1）用H67细胞系确定（I）纯化的鼠抗体的N-末端氨基酸序列，（2）PCR反应来自 H67与重链引物作为5'-CCC GAA TTC GAG GT（TC）CAG CTG C​​A（GA）CA（GA）TCT GG（GA）TCT GG（GA）CCT G-3'和5-CGG TCG ACA GGG ATC CAG AGT TCC AGG GTC AC-3'获得质粒pMHV-S（KCTC 0044BP），（3​​）与（2）相同的轻链作为5'-CCC GAA TTC GAC ATT GTG CTG AC（CA）CA ）TCT CC（TA）GCT-3'和5'-CCC GCC TTG GAC ATT GTG CTG AC（CA）CA（GA）TCT CC（TA）GCT-3'获得质粒pMKV-S（KCTC 0045BP） （4）测序克隆的基因。

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