公开(公告)号：CA2645758C

公开(公告)日：2016-08-09

申请号：CA2645758

申请日：2007-03-29

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： RANK DAVID R ,  WEGENER JEFFERY ,  KORLACH JONAS ,  ROITMAN DANIEL ,  XU YUE ,  LYLE JOHN ,  TURNER STEPHEN ,  PELUSO PAUL ,  OTTO GEOFF ,  CICERO RONALD L

IPC: C12N11/00 ,  C40B40/04

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

公开(公告)号：AU2010245304B2

公开(公告)日：2015-06-04

申请号：AU2010245304

申请日：2010-04-26

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： KORLACH JONAS ,  TURNER STEPHEN ,  SCHADT ERIC

IPC: C12N15/10 ,  C12Q1/68

Abstract: The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of a variety of different biological reactions. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to either enhance or inhibit such reactions. In certain preferred embodiments, RNA templates are used in single-molecule real-time sequencing reactions.

公开(公告)号：AU2009325069B2

公开(公告)日：2015-03-19

申请号：AU2009325069

申请日：2009-12-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  TURNER STEPHEN ,  LEE JESSICA ,  JIA LEI ,  KORLACH JONAS ,  SORENSON JON ,  WEBSTER DALE ,  LYLE JOHN ,  TRAVERS KEVIN ,  HANES JEREMIAH

IPC: C12Q1/68 ,  C12N15/10

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：GB2517875A

公开(公告)日：2015-03-04

申请号：GB201500009

申请日：2013-06-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： TURNER STEPHEN ,  KORLACH JONAS

IPC: C12Q1/68

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. Nanopore sequencing is performed using a processive enzyme to control the rate of translation of a single strand of a nucleic acid through a nanopore. The presence and identity of a modified base can be determined by monitoring the kinetics of translation through the pore even though the events that lead to the kinetic changes are separated in time and space from the translation of the modified base or it's compliment through the nanopore. The invention also comprises the use of hemi-genomic DNA in nanopore sequencing.

公开(公告)号：CA2654470C

公开(公告)日：2015-02-03

申请号：CA2654470

申请日：2007-06-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： FOQUET MATHIEU ,  PELUSO PAUL ,  TURNER STEPHEN ,  ROITMAN DANIEL BERNARDO ,  OTTO GEOFFREY

IPC: G02B6/10 ,  B82Y15/00 ,  G01N21/77 ,  G01N33/48

Abstract: Substrates, including zero mode waveguide substrates that have been fabricated to provide additional f.upsilon..pi.clionai elements and/or components including increased volumes for positioning of active surfaces and or components for the mitigation of negative electrochemical properties of the underlying substrates.

公开(公告)号：AU2009300369B2

公开(公告)日：2014-08-14

申请号：AU2009300369

申请日：2009-09-25

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZACCARIN DENIS ,  LUNDQUIST PAUL ,  ZHAO PEIQIAN ,  ZHONG FRANK ,  TURNER STEPHEN ,  HUANG YANQIAO ,  MONADGEMI PEZHMAN ,  SAXENA RAVI ,  GROT ANNETTE ,  RULISON AARON

IPC: G01N21/00 ,  G02B6/12

Abstract: Apparatus, systems and methods for use in analyzing discrete reactions at ultra high multiplex with reduced optical noise, and increased system flexibility. Apparatus include substrates having integrated optical components that increase multiplex capability by one or more of increasing density of reaction regions, improving transmission of light to or collection of light from discrete reactions regions. Integrated optical components include reflective optical elements which re-direct illumination light and light emitted from the discrete regions to more efficiently collect emitted light. Particularly preferred applications include single molecule reaction analysis, such as polymerase mediated template dependent nucleic acid synthesis and sequence determination.

公开(公告)号：AU2007215242B2

公开(公告)日：2012-10-18

申请号：AU2007215242

申请日：2007-02-09

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZACCARIN DENIS ,  FOQUET MATHIEU ,  MAXHAM MARK ,  TURNER STEPHEN ,  LUNDQUIST PAUL ,  LACROIX YVES

IPC: F21V9/16 ,  G01J1/58 ,  G01T1/10 ,  G21H3/02 ,  G21K5/00

Abstract: Methods and systems for real-time monitoring of optical signals from arrays of signal sources, and particularly optical signal sources that have spectrally different signal components. Systems include signal source arrays in optical communication with optical trains that direct excitation radiation to and emitted signals from such arrays and image the signals onto detector arrays, from which such signals may be subjected to additional processing.

公开(公告)号：CA2817587A1

公开(公告)日：2012-05-18

申请号：CA2817587

申请日：2011-11-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： FLUSBERG BENJAMIN ,  KORLACH JONAS ,  WEGENER JEFFREY ,  CLARK TYSON A ,  VILFAN IGOR ,  KISLYUK ANDREY ,  TURNER STEPHEN ,  SORENSON JON ,  TRAVERS KEVIN ,  HEINER CHERYL ,  TOMANEY AUSTIN B ,  MARKS PATRICK ,  LEE JESSICA ,  JIA LEI ,  WEBSTER DALE ,  LYLE JOHN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/48

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：CA2790393A1

公开(公告)日：2011-08-25

申请号：CA2790393

申请日：2011-02-18

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： MCCAFFREY NATHANIEL JOSEPH ,  TURNER STEPHEN ,  SAXENA RAVI ,  HELGESEN SCOTT EDWARD

IPC: G01N21/00

Abstract: An analytical assembly within a unified device structure for integration into an analytical system. The analytical assembly is scalable and includes a plurality of analytical devices, each of which includes a reaction cell, an optical sensor, and at least one optical element positioned in optical communication with both the reaction cell and the sensor and which delivers optical signals from the cell to the sensor. Additional elements are optionally integrated into the analytical assembly. Methods for forming and operating the analytical system are also disclosed.

公开(公告)号：AU2006213692B2

公开(公告)日：2011-03-24

申请号：AU2006213692

申请日：2006-02-09

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： TURNER STEPHEN ,  KORLACH JONAS

IPC: C12P19/34