公开(公告)号：CA2596495A1

公开(公告)日：2006-08-17

申请号：CA2596495

申请日：2006-02-09

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： TURNER STEPHEN ,  KORLACH JONAS

IPC: G01N31/00

公开(公告)号：WO2012065043A9

公开(公告)日：2012-12-20

申请号：PCT/US2011060338

申请日：2011-11-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  FLUSBERG BENJAMIN ,  KORLACH JONAS ,  WEGENER JEFFREY ,  CLARK TYSON A ,  VILFAN IGOR ,  KISLYUK ANDREY ,  TURNER STEPHEN ,  SORENSON JON ,  TRAVERS KEVIN ,  HEINER CHERYL ,  TOMANEY AUSTIN B ,  MARKS PATRICK ,  LEE JESSICA ,  JIA LEI ,  WEBSTER DALE ,  LYLE JOHN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

Inventor： FLUSBERG BENJAMIN ,  KORLACH JONAS ,  WEGENER JEFFREY ,  CLARK TYSON A ,  VILFAN IGOR ,  KISLYUK ANDREY ,  TURNER STEPHEN ,  SORENSON JON ,  TRAVERS KEVIN ,  HEINER CHERYL ,  TOMANEY AUSTIN B ,  MARKS PATRICK ,  LEE JESSICA ,  JIA LEI ,  WEBSTER DALE ,  LYLE JOHN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/48

CPC classification number: C12Q1/6869 ,  C12Q1/6837 ,  C12Q1/6858 ,  C12Q1/6874 ,  C12Q1/689 ,  C12Q2600/154 ,  C12Q2537/164 ,  C12Q2527/113 ,  C12Q2521/101 ,  C12Q2525/307 ,  C12Q2525/117

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

Abstract translation: 提供了用于表征修饰的核酸的方法，组合物和系统。 在某些优选的实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰包括化学修饰的碱基，酶修饰的碱基，无碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。

公开(公告)号：WO2012065043A3

公开(公告)日：2012-08-16

申请号：PCT/US2011060338

申请日：2011-11-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  FLUSBERG BENJAMIN ,  KORLACH JONAS ,  WEGENER JEFFREY ,  CLARK TYSON A ,  VILFAN IGOR ,  KISLYUK ANDREY ,  TURNER STEPHEN ,  SORENSON JON ,  TRAVERS KEVIN ,  HEINER CHERYL ,  TOMANEY AUSTIN B ,  MARKS PATRICK ,  LEE JESSICA ,  JIA LEI ,  WEBSTER DALE ,  LYLE JOHN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

Inventor： FLUSBERG BENJAMIN ,  KORLACH JONAS ,  WEGENER JEFFREY ,  CLARK TYSON A ,  VILFAN IGOR ,  KISLYUK ANDREY ,  TURNER STEPHEN ,  SORENSON JON ,  TRAVERS KEVIN ,  HEINER CHERYL ,  TOMANEY AUSTIN B ,  MARKS PATRICK ,  LEE JESSICA ,  JIA LEI ,  WEBSTER DALE ,  LYLE JOHN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/48

CPC classification number: C12Q1/6869 ,  C12Q1/6837 ,  C12Q1/6858 ,  C12Q1/6874 ,  C12Q1/689 ,  C12Q2600/154 ,  C12Q2537/164 ,  C12Q2527/113 ,  C12Q2521/101 ,  C12Q2525/307 ,  C12Q2525/117

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

Abstract translation: 提供了方法，组合物和系统用于表征修饰的核酸。 在某些优选实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基，酶修饰的碱基，脱碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。

公开(公告)号：WO2010027484A2

公开(公告)日：2010-03-11

申请号：PCT/US2009004993

申请日：2009-09-04

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  PATEL PRANAV ,  KORLACH JONAS ,  BIBILLO ARKADIUSZ ,  BJORNSON KEITH ,  HANES JEREMIAH

Inventor： PATEL PRANAV ,  KORLACH JONAS ,  BIBILLO ARKADIUSZ ,  BJORNSON KEITH ,  HANES JEREMIAH

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/25

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Q2527/113 ,  C12Q2521/101 ,  C12Q2527/137 ,  C12Q2537/161 ,  C12Q2525/101 ,  C12Q2563/107

Abstract: Provided are methods for enhanced sequencing of nucleic acid templates. Also provided are reaction conditions that increase branching fractions during polymerization reactions. Also provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are higher than the branching fractions of the polymerases from which they were derived. Provided are compositions comprising modified recombinant polymerases that exhibit delayed translocation relative to the polymerases from which they were derived. Also provided are compositions comprising modified recombinant polymerases that exhibit increased nucleotide or nucleotide analog residence time at an active site of the polymerase. Provided are methods for generating polymerases with the aforementioned phenotypes and methods of using such polymerases to sequence a DNA template or make a DNA. Also provided are methods and nucleic acid sequencing systems for determining which labeled nucleotide is incorporated at a site during a template-dependent polymerization reaction.

Abstract translation: 提供了用于增强核酸模板测序的方法。 还提供了在聚合反应期间增加支化馏分的反应条件。 还提供了包含改性重组聚合酶的组合物，其表现出高于其衍生的聚合酶的支化部分的支化部分。 提供了包含修饰的重组聚合酶的组合物，其相对于衍生自其的聚合酶表现出延迟的移位。 还提供了包含修饰的重组聚合酶的组合物，其在聚合酶的活性位点表现出增加的核苷酸或核苷酸类似物停留时间。 提供了使用上述表型产生聚合酶的方法和使用这种聚合酶来顺序DNA模板或制备DNA的方法。 还提供了用于在模板依赖性聚合反应期间确定哪个标记的核苷酸被掺入位点的方法和核酸测序系统。

公开(公告)号：CA2746632C

公开(公告)日：2020-06-30

申请号：CA2746632

申请日：2009-12-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  TURNER STEPHEN ,  LEE JESSICA ,  JIA LEI ,  KORLACH JONAS ,  SORENSON JON ,  WEBSTER DALE ,  LYLE JOHN ,  TRAVERS KEVIN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

IPC: C12Q1/68 ,  C12Q1/6809 ,  C12Q1/6869

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：CA2737505C

公开(公告)日：2017-08-29

申请号：CA2737505

申请日：2009-09-15

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZHONG CHENG FRANK ,  LUNDQUIST PAUL ,  FOQUET MATHIEU ,  KORLACH JONAS ,  BAYANDORIAN HOVIG

IPC: G01N21/00 ,  G01B9/02 ,  G01N33/483

Abstract: This invention provides substrates for use in various applications, including single-molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided.

公开(公告)号：AU2014203354B2

公开(公告)日：2016-06-30

申请号：AU2014203354

申请日：2014-06-20

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZHONG CHENG FRANK ,  LUNDQUIST PAUL ,  FOQUET MATHIEU ,  KORLACH JONAS ,  BAYANDORIAN HOVIG

IPC: G01N21/00 ,  G01B9/02 ,  G01N33/483

Abstract: This invention provides substrates for use in various applications, including single molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided. ocdq N"t c 00 (Suonlw)o . o

公开(公告)号：AU2014203354A1

公开(公告)日：2014-07-10

申请号：AU2014203354

申请日：2014-06-20

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： ZHONG CHENG FRANK ,  LUNDQUIST PAUL ,  FOQUET MATHIEU ,  KORLACH JONAS ,  BAYANDORIAN HOVIG

IPC: G01N21/00 ,  G01B9/02 ,  G01N33/483

Abstract: This invention provides substrates for use in various applications, including single molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided. ocdq N"t c 00 (Suonlw)o . o

公开(公告)号：ES2385015T3

公开(公告)日：2012-07-17

申请号：ES07754529

申请日：2007-03-29

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： RANK DAVID R ,  WEGENER JEFFERY ,  KORLACH JONAS ,  ROITMAN DANIEL ,  XU YUE ,  LYLE JOHN ,  TURNER STEPHEN ,  PELUSO PAUL ,  OTTO GEOFF ,  CICERO RONALD L

IPC: C12N11/00 ,  C40B40/04

Abstract: Un método de proporcionar un sustrato que tiene una enzima inmovilizada selectivamente sobre él, comprendiendoel método:proporcionar un sustrato que tiene un primer componente de la superficie que comprende SiO2 y un segundocomponente de la superficie que comprende un metal u óxido metálico;poner en contacto el sustrato con una primera composición que se asocia selectivamente con el primer componentede la superficie, comprendiendo dicha primera composición un silano;acoplar la enzima a la primera composición; yponer en contacto el sustrato con una segunda composición que se asocia selectivamente con el segundocomponente de la superficie, comprendiendo dicha segunda composición un compuesto que comprende uno o másgrupos ácido fosfónico o fosfato.

公开(公告)号：AU2006320739B2

公开(公告)日：2012-03-29

申请号：AU2006320739

申请日：2006-11-27

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： KORLACH JONAS

IPC: C23C16/00

Abstract: Devices, systems and methods of using same where hybrid substrate materials are provided with a substantially uniform surface to provide uniformity of properties, including interaction with their environments. Uniform surfaces are applied as coatings over, e.g., hybrid metal/silica, metal/polymer, metal/metal surfaces to mask different chemical properties of differing regions of the surface and to afford a protective surface for the hybrid structure.