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公开(公告)号：KR1020050108109A
公开(公告)日：2005-11-16
申请号：KR1020040033170
申请日：2004-05-11
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N1/20
Abstract: 본 발명은 엔테로코커스 에스피 58 (KACC 91099) 및 이의 용도에 관한 것으로서, 상기 균주는 내산성, 내담즙성 및 생체 안전성이 인정되고, 병원성 세균 억제활성을 가지며, 장내에서 독립된 균총(Niche)으로서의 활성과 이에 근거한 길항 작용을 통한 병원성 균총의 증식을 감소시켜 질병을 예방하는 효과가 있다.
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52.发明授权수포성 구내염 바이러스의 외피당단백질 항원 및 그에대한 모노클로날 항체를 이용하는 수포성 구내염의 진단방법 有权수포성구내염바이러스의외피당단백질항원및그에대한모노클로날항체를이용하는수포성구내염의진단방
公开(公告)号：KR100449908B1
公开(公告)日：2004-09-22
申请号：KR1020010082714
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/12
Abstract: PURPOSE: A method of diagnosing vesicular stomatitis by performing an ELISA method using the recombinant envelope glycoprotein antigen of vesicular stomatitis virus(VSV) and monoclonal antibodies thereon is provided. Therefore, it permits quick, exact and specific antibody detection of the VSV and thus exact diagnosis of vesicular stomatitis through the detection. CONSTITUTION: The diagnosing method for vesicular stomatitis is achieved through a VSV detection method consisting of: attaching monoclonal antibodies against an envelope glycoprotein antigen of VSV on a solid supporter; binding the envelope glycoprotein antigen to the monoclonal antibodies attached on the solid supporter in the first step; reacting a sample containing an antibody against the envelope glycoprotein antigen with the envelope glycoprotein antigen of the second step; reacting a secondary antibody selected from the group consisting of a conjugate antibody attached with enzyme and a conjugate antibody attached with a luminescent material with the antibody of the third step.
Abstract translation: 目的：提供一种使用水疱性口炎病毒（VSV）的重组包膜糖蛋白抗原和单克隆抗体进行ELISA方法来诊断水疱性口炎的方法。 因此，它可以快速，准确和特异地检测VSV的抗体，从而通过检测来精确诊断水疱性口炎。 组成：水泡性口炎的诊断方法通过VSV检测方法实现，该方法包括：将抗VSV的包膜糖蛋白抗原的单克隆抗体附着在固体支持物上; 在第一步中将包膜糖蛋白抗原结合至附着于固体载体上的单克隆抗体; 使含有抗包膜糖蛋白抗原的抗体的样品与第二步的包膜糖蛋白抗原反应; 使选自附着有酶的缀合物抗体和附着有荧光物质的缀合物抗体的第二抗体与第三步骤的抗体反应。
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公开(公告)号：KR100449906B1
公开(公告)日：2004-09-22
申请号：KR1020010082977
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
Abstract: PURPOSE: A diagnostic method of foot-and-mouth disease using a recombinant FMCV 2C non-structural protein and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant FMCV 2C non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant vector is prepared by cloning the gene encoding the recombinant FMCV 2C non-structural protein of SEQ ID NO: 1. The recombinant FMCV 2C non-structural protein is expressed by transformation of a cell with the recombinant vector. A hybridoma cell line(KCTC 10137BP) is prepared by introducing the recombinant FMCV 2C non-structural protein into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A recombinant FMCV 2C non-structural protein specific monoclonal antibody is produced from the hybridoma cell line(KCTC 10137BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant FMCV 2C non-structural protein specific monoclonal antibody in a coating buffer solution and pouring the diluate on a plate; (2) washing the plate to remove unattached monoclonal antibodies; (3) reacting the recombinant FMCV 2C non-structural protein with the plate; (4) washing the plate to remove unreacted recombinant FMCV 2C non-structural proteins; (5) reacting the testing serum with the plate; (6) washing the plate to remove unreacted testing serum; (7) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (8) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用重组FMCV 2C非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速和更准确地诊断口蹄疫。 构成：编码来源于韩国口蹄疫病毒O / SKR / 2000的重组FMCV 2C非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。重组载体通过克隆编码 重组FMCV 2C非结构蛋白SEQ ID NO：1。通过用重组载体转化细胞表达重组FMCV 2C非结构蛋白。 通过将重组FMCV 2C非结构蛋白导入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系（KCTC 10137BP）。 从杂交瘤细胞系（KCTC 10137BP）产生重组FMCV 2C非结构蛋白特异性单克隆抗体。 一种口蹄疫诊断方法，包括以下步骤：（1）将重组FMCV 2C非结构蛋白特异性单克隆抗体稀释于包被缓冲液中，并将稀释液倒入平板中; （2）清洗平板以去除未附着的单克隆抗体; （3）使重组FMCV 2C非结构蛋白与平板反应; （4）洗涤平板以除去未反应的重组FMCV 2C非结构蛋白; （5）使测试血清与平板反应; （6）洗涤平板以除去未反应的测试血清; （7）使与试验血清中的口蹄疫病毒抗体结合的缀合物与试验血清具有酶，放射性物质或荧光物质的步骤; 和（8）测量酶反应的强度，与缀合物的荧光反应或辐射反应。
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公开(公告)号：KR100430935B1
公开(公告)日：2004-05-14
申请号：KR1020010082975
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using a recombinant 3ABC non-structural protein expressed in E. coli and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed from a recombinant E. coli transformed with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the dilution on a plate; (2) reacting the testing serum with the plate; (3) reacting the 3ABC non-structural protein specific monoclonal antibody with the serum; (4) reacting a 3ABC non-structural protein specific monoclonal antibody binding conjugate with the monoclonal antibody; and (5) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用在大肠杆菌中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速和更准确地诊断口蹄疫。 构成：编码来源于韩国口蹄疫病毒O / SKR / 2000的重组3ABC非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。重组3ABC非结构蛋白由重组 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体转化大肠杆菌。通过引入在E.coli中表达的重组3ABC非结构蛋白制备杂交瘤细胞系3F-11（KCTC 10138BP） 将大肠杆菌导入动物体内，从动物体内收集免疫细胞，并将免疫细胞与癌细胞融合。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 口蹄疫诊断方法包括以下步骤：（1）将重组3ABC非结构蛋白稀释于包被缓冲液中，并将稀释液倒入板中; （2）使测试血清与平板反应; （3）使3ABC非结构蛋白特异性单克隆抗体与血清反应; （4）使3ABC非结构蛋白特异性单克隆抗体结合缀合物与单克隆抗体反应; 和（5）测量酶反应的强度，与偶联物的荧光反应或辐射反应。
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公开(公告)号：KR1020030053857A
公开(公告)日：2003-07-02
申请号：KR1020010083880
申请日：2001-12-24
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C07K19/00
CPC classification number: C07K14/44 , A61K39/002 , A61K2039/552 , C07K2319/00 , C12N15/62 , C12N15/70
Abstract: PURPOSE: A fusion protein having enhanced immunogenicity to bovine theileriosis, a preparation process thereof, and the use thereof are provided, thereby preventing the infection of bovine theileriosis. CONSTITUTION: A fusion protein having enhanced immunogenicity to bovine theileriosis is prepared by fusion of heat shock protein(HSP) with the amino-terminal of the surface antigen p33 of Theileria sergenti, wherein Theileria sergenti is Korean isolated Theileria sergenti, Sungwhan stock; the surface antigen p33 has the nucleotide sequence of SEQ ID NO: 1; and the heat shock protein(HSP) has the nucleotide sequence of SEQ ID NO: 2. A process for preparing the fusion protein having enhanced immunogenicity to bovine theileriosis comprises the steps of: constructing a recombinant vector containing the gene encoding the fusion protein of heat shock protein(HSP) and the surface antigen p33 of Theileria sergenti; preparing a transformed E. coli with the recombinant vector; and culturing the transformed E. coli.
Abstract translation: 目的：提供具有增强的牛鳞状细胞病毒免疫原性的融合蛋白及其制备方法及其用途，从而防止牛皮肤病的感染。 构成：通过将热休克蛋白（HSP）与Theileria sergenti的表面抗原p33的氨基末端融合制备具有增强的牛鳞状细胞病毒免疫原性的融合蛋白，其中Theileria sergenti是韩国分离的Theileria sergenti，Sungwhan stock; 表面抗原p33具有SEQ ID NO：1的核苷酸序列; 热休克蛋白（HSP）具有SEQ ID NO：2的核苷酸序列。一种制备具有增强的牛鳞状细胞病免疫原性的融合蛋白的方法，包括以下步骤：构建含有编码热能融合蛋白的基因的重组载体 休克蛋白（HSP）和沙门氏菌的表面抗原p33; 用重组载体制备转化的大肠杆菌; 并培养转化的大肠杆菌。
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Patent Agency Ranking
公开(公告)号：KR1020030052859A
公开(公告)日：2003-06-27
申请号：KR1020010082977
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
CPC classification number: C12N5/163 , C07K16/1009 , C07K2317/14 , C12N2770/32131 , G01N33/56983 , G01N33/577 , G01N2333/09
Abstract: PURPOSE: A diagnostic method of foot-and-mouth disease using a recombinant FMCV 2C non-structural protein and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant FMCV 2C non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant vector is prepared by cloning the gene encoding the recombinant FMCV 2C non-structural protein of SEQ ID NO: 1. The recombinant FMCV 2C non-structural protein is expressed by transformation of a cell with the recombinant vector. A hybridoma cell line(KCTC 10137BP) is prepared by introducing the recombinant FMCV 2C non-structural protein into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A recombinant FMCV 2C non-structural protein specific monoclonal antibody is produced from the hybridoma cell line(KCTC 10137BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant FMCV 2C non-structural protein specific monoclonal antibody in a coating buffer solution and pouring the diluate on a plate; (2) washing the plate to remove unattached monoclonal antibodies; (3) reacting the recombinant FMCV 2C non-structural protein with the plate; (4) washing the plate to remove unreacted recombinant FMCV 2C non-structural proteins; (5) reacting the testing serum with the plate; (6) washing the plate to remove unreacted testing serum; (7) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (8) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用重组FMCV 2C非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速，准确地诊断口蹄疫。 构成：编码来自韩国口蹄疫病毒O / SKR / 2000的重组FMCV 2C非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。通过克隆编码 SEQ ID NO：1的重组FMCV 2C非结构蛋白。通过用重组载体转化细胞来表达重组FMCV 2C非结构蛋白。 通过将重组FMCV 2C非结构蛋白引入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合，来制备杂交瘤细胞系（KCTC 10137BP）。 从杂交瘤细胞系（KCTC 10137BP）产生重组FMCV 2C非结构蛋白特异性单克隆抗体。 口蹄疫的诊断方法包括以下步骤：（1）在包衣缓冲溶液中稀释重组FMCV 2C非结构蛋白特异性单克隆抗体，将稀释物倒入板上; （2）洗涤板以除去未附着的单克隆抗体; （3）使重组FMCV 2C非结构蛋白与板反应; （4）洗涤板以除去未反应的重组FMCV 2C非结构蛋白; （5）使测试血清与板反应; （6）洗板以除去未反应的检测血清; （7）使与测试血清中的口蹄疫病毒抗体结合并具有酶，放射性物质或荧光材料的缀合物与测试血清反应; 和（8）测量酶反应的强度，荧光反应或与缀合物的辐射反应。
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57.发明公开대장균 발현 재조합 3ＡＢＣ 비구조단백질 및단클론항체를 이용한 구제역 진단방법 有权使用在大肠杆菌和单克隆抗体中表达的重组体3ABC非结构蛋白的FOOT-AND-MOUTH DISEASE的诊断方法
公开(公告)号：KR1020030052857A
公开(公告)日：2003-06-27
申请号：KR1020010082975
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
CPC classification number: C07K14/005 , C07K16/1009 , C07K2317/14 , C12N5/163 , C12N15/70 , C12N2770/32131 , G01N33/56983 , G01N33/577 , G01N2333/09
Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using a recombinant 3ABC non-structural protein expressed in E. coli and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed from a recombinant E. coli transformed with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the dilution on a plate; (2) reacting the testing serum with the plate; (3) reacting the 3ABC non-structural protein specific monoclonal antibody with the serum; (4) reacting a 3ABC non-structural protein specific monoclonal antibody binding conjugate with the monoclonal antibody; and (5) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用在大肠杆菌中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫的诊断方法，从而比现有方法更快速和准确地诊断口蹄疫。 构成：编码来自韩国口蹄疫病毒O / SKR / 2000的重组3ABC非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。重组体3ABC非结构蛋白由重组体 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体转化大肠杆菌。通过引入在E中表达的重组3ABC非结构蛋白来制备杂交瘤细胞系3F-11（KCTC 10138BP）。 将大肠杆菌转化成动物，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 口蹄疫的诊断方法包括以下步骤：（1）在包被缓冲溶液中稀释重组体3ABC非结构蛋白，并将稀释液倒在平板上; （2）使测试血清与板反应; （3）使3ABC非结构蛋白特异性单克隆抗体与血清反应; （4）使3ABC非结构蛋白特异性单克隆抗体结合缀合物与单克隆抗体反应; 和（5）测量酶反应的强度，荧光反应或与缀合物的辐射反应。
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公开(公告)号：KR1019990085154A
公开(公告)日：1999-12-06
申请号：KR1019980017349
申请日：1998-05-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N7/02
Abstract: 본 발명은 주요 돼지 전염성 질병의 원인체로 돼지 코로나바이러스군인 유행성 설사병 바이러스, 전염성 위장염 바이러스 및 호흡기 코로나바이러스를 신속하고, 간편하게 고역가로 정제하는 방법에 관한 것으로, 본 발명에 의해 정제된 바이러스는 면역제제 작성이나 ELISA법, Dip-stick법, dot blot법 등에 적용되어 바이러스의 진단에 이용될 수 있으며, 고효능의 예방약 생산 및 야외에서 손쉽게 적용할 수 있는 진단키트의 생산에도 사용될 수 있다.
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公开(公告)号：KR101457977B1
公开(公告)日：2014-11-04
申请号：KR1020120090768
申请日：2012-08-20
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/86 , C12N15/33 , C12N5/10 , G01N33/569
Abstract: 본 발명은 돼지 E형 간염 바이러스의 캡시드 단백질을 발현하는 재조합 세포에 관한 것으로, 실험실 내에서 인공배양이 어려운 돼지 E형 간염 바이러스의 감염 상태와 매우 유사한 캡시드 단백질을 생산하여 돼지 E형 간염 바이러스에 대한 백신으로서 유용하게 사용할 수 있고, 바이러스의 인공 배양이 어려운 특성을 고려할때, 상기 캡시드 단백질을 최종 확진 항체검사 방법에 이용할 수 있어 정확한 질병 진단에 매우 유용하다.
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公开(公告)号：KR1020140081368A
公开(公告)日：2014-07-01
申请号：KR1020120151034
申请日：2012-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: The present invention relates to a composition for diagnosing Korean sacbrood virus infection and a method for diagnosing Korean sacbrood using the same. A primer set for diagnosing Korean sacbrood virus infection according to the present invention exhibits excellent effects of detecting the Korean sacbrood virus in 10 minutes at high specificity and sensitivity in various sacbrood virus infection samples, and thus can be effectively used in rapid detection and diagnosis of the Korean sacbrood virus.
Abstract translation: 本发明涉及用于诊断韩国囊状病毒感染的组合物以及使用该组合物来诊断韩国sacbrood的方法。 根据本发明的用于诊断韩国囊状病毒感染的引物组在各种囊状病毒感染样品中以高特异性和灵敏度在10分钟内检测韩国囊状病毒具有优异的效果，因此可以有效地用于快速检测和诊断 韩国sacbrood病毒。
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