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公开(公告)号：CA2735979A1
公开(公告)日：2010-03-11
申请号：CA2735979
申请日：2009-09-04
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： PATEL PRANAV , KORLACH JONAS , BIBILLO ARKADIUSZ , BJORNSON KEITH , HANES JEREMIAH
Abstract: Provided are methods for enhanced sequencing of nucleic acid templates. Also provided are reaction conditions that increase branching fractions during polymerization reactions. Also provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are higher than the branching fractions of the polymerases from which they were derived. Provided are compositions comprising modified recombinant polymerases that exhibit delayed translocation relative to the polymerases from which they were derived. Also provided are compositions comprising modified recombinant polymerases that exhibit increased nucleotide or nucleotide analog residence time at an active site of the polymerase. Provided are methods for generating polymerases with the aforementioned phenotypes and methods of using such polymerases to sequence a DNA template or make a DNA. Also provided are methods and nucleic acid sequencing systems for determining which labeled nucleotide is incorporated at a site during a template-dependent polymerization reaction.
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公开(公告)号：CA2718404A1
公开(公告)日：2009-09-17
申请号：CA2718404
申请日：2009-03-12
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： WEGENER JEFFREY , KORLACH JONAS
IPC: G01N33/532 , C12Q1/68 , G01N33/53 , G01N33/533 , G01N33/535
Abstract: Labeled reactant compositions, and particularly labeled nucleic acid reaction compositions, that include structural components that maintain potentially damaging labeling components sufficiently distal from the reactant portion of the molecule such that damaging effects of the label group on other reaction components, such as enzymes, are reduced, minimized and/or eliminated.
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公开(公告)号：CA2707600A1
公开(公告)日：2009-06-11
申请号：CA2707600
申请日：2008-12-03
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： KORLACH JONAS , ROITMAN DANIEL , EID JOHN , OTTO GEOFF , HARDENBOL PAUL , FLUSBERG BENJAMIN
IPC: G01N33/573 , G01N33/53 , G01N33/535
Abstract: Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.
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公开(公告)号：AU2006331677A1
公开(公告)日：2007-07-05
申请号：AU2006331677
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： RANK DAVID , KORLACH JONAS , HANZEL DAVID , PHAM THANG , OTTO GEOFF , TURNER STEPHEN , PELUSO PAUL
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公开(公告)号：AU2006331512A1
公开(公告)日：2007-07-05
申请号：AU2006331512
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： KORLACH JONAS , OTTO GEOFFREY , HANZEL DAVID , PELUSO PAUL , TURNER STEPHEN , RANK DAVID , PHAM THANG
Abstract: Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.
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Patent Agency Ranking
公开(公告)号：CA2631248A1
公开(公告)日：2007-06-07
申请号：CA2631248
申请日：2006-11-27
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： KORLACH JONAS
IPC: C23C16/00
Abstract: Devices, systems and methods of using same where hybrid substrate materials are provided with a substantially uniform surface to provide uniformity of properties, including interaction with their environments. Uniform surfaces are applied as coatings over, e.g., hybrid metal/silica, metal/polymer, metal/metal surfaces to mask different chemical properties of differing regions of the surface and to afford a protective surface for the hybrid structure.
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公开(公告)号：GB2423819A
公开(公告)日：2006-09-06
申请号：GB0608338
申请日：2005-09-16
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： TURNER STEPHEN , KORLACH JONAS
Abstract: The present invention relates to optical confinements, method of preparing and methods of using them for analyzing molecules and/or monitoring chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost single-molecular analysis.
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公开(公告)号：CA2579150A1
公开(公告)日：2006-04-27
申请号：CA2579150
申请日：2005-09-16
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： KORLACH JONAS , TURNER STEPHEN W
IPC: H05H3/04
Abstract: The present invention relates to optical confinements, method of preparing and methods of using them for analyzing molecules and/or monitoring chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost single-molecular analysis.
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公开(公告)号：AU2005296200A1
公开(公告)日：2006-04-27
申请号：AU2005296200
申请日：2005-09-16
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： TURNER STEPHEN , KORLACH JONAS
IPC: H05H3/04
Abstract: The present invention relates to optical confinements, method of preparing and methods of using them for analyzing molecules and/or monitoring chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost single-molecular analysis.
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公开(公告)号：EP2331934A4
公开(公告)日：2015-04-01
申请号：EP09814888
申请日：2009-09-15
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： ZHONG CHENG FRANK , LUNDQUIST PAUL , FOQUET MATHIEU , KORLACH JONAS , BAYANDORIAN HOVIG
CPC classification number: C12Q1/6874 , C12Q1/68 , C12Q1/6825 , G01N21/6428 , G01N21/648 , G01N21/7703 , G01N33/54373 , G01N2021/6463 , G01N2201/06113 , G01N2201/0638 , G01N2201/08
Abstract: This invention provides substrates for use in various applications, including single-molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided.
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