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1.发明公开Ｌａｃｔｏｃｏｃｃｕｓ ｌａｃｔｉｓ ＮＫ34 ＫＦＣＣ 11458Ｐ의 배지 최적화를 통한 젖소 유방염 원인균에 효과가 있는 ｌａｃｔｉｃｉｎ ＮＫ34의 생산방법 无效新型LACTICIN NK34及其生产方法，通过优化培养液对乳腺发炎引起的微生物有效
公开(公告)号：KR1020110017241A
公开(公告)日：2011-02-21
申请号：KR1020090074809
申请日：2009-08-13
Applicant: 건국대학교 산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장) , 서울대학교산학협력단
Abstract: PURPOSE: A Lactococcus lactis NK34 for producing bacteriocin lacticin NK34 having antibacterial effect to bovine mastitis bacteria is provided to replace antibiotics and to produce a large amount of lacticin NK34. CONSTITUTION: A Lactococcus lactis NK34 strain is purely cultured in MRS medium and produces bacteriocin lacticin NK34 having an effect of suppressing bovine mastitis causing bacteria. The Lactococcus lactis NK34 KFCC 11458P is cultured in a medium containing sucrose, tryptone, yeast extract, tween 80, sodium acetate, and NaCl. A medium composition for culturing Lactococcus lactis NK34 KFCC 11458P or performing biosynthesis of bacteriocin lactinic NK34 contains sucrose, tryptone, yeast extract, tween 80, sodium acetate, and NaCl.
Abstract translation: 目的：提供乳酸乳球菌NK34，用于产生对牛乳杆菌细菌具有抗菌作用的细菌素乳酸菌NK34，以代替抗生素并产生大量乳酸菌NK34。 构成：乳酸乳球菌NK34菌株纯化在MRS培养基中培养，产生具有抑制牛乳腺炎引起细菌的作用的细菌素乳酸菌NK34。 乳酸乳球菌NK34 KFCC 11458P在含有蔗糖，胰蛋白胨，酵母提取物，吐温80，乙酸钠和NaCl的培养基中培养。 用于培养乳酸乳球菌NK34KFCC11458P或进行细菌素乳内酯NK34的生物合成的培养基组合物含有蔗糖，胰蛋白胨，酵母提取物，吐温80，乙酸钠和NaCl。
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公开(公告)号：KR1020100059668A
公开(公告)日：2010-06-04
申请号：KR1020090075672
申请日：2009-08-17
Applicant: 건국대학교 산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C07K14/195 , C12N1/20 , C12P21/00
Abstract: PURPOSE: A bacteriocin which is derived from Lactococcus lactis NK34 KFCC 11458P and a therapeutic agent for breast cancer using the same are provided to ensure antibacterial activity and to be used as an antibiotic substitute. CONSTITUTION: A Lactcin NK34 enzyme is isolated from Lactococcus lactis NK34 KFCC 11458P and is used for treating breast cancer of dairy cattle. The Lacticin NK34 has an antibacterial effect to S. uberis E290, S. agalactiae ATCC 13813, E. gallinarum E362, E. faecium E363, E. faecium E374 and P. autotrophica KCTC 9455. A method for isolating bacteriocin Lacticin NK34 enzyme comprises: a step of isolating and culturing using a strain; a step of measuring antibacterial activity of the bacteriocin; a step of researching the characteristics of Lactin NK 34 enzyme; and a step of measuring antibacterial effects to a breast cancer pathogenic bacteria.
Abstract translation: 目的：提供来源于乳酸乳球菌NK34 KFCC 11458P的细菌素和使用其的乳腺癌治疗剂以确保抗菌活性并用作抗生素替代物。 构成：乳酸菌NK34酶从乳酸乳球菌NK34 KFCC 11458P分离，用于治疗乳牛的乳腺癌。 乳酸NK34具有对乳房链球菌E290，无乳链球菌ATCC 13813，gall藜E362，屎肠球菌E363，屎肠球菌E374和自交亲属KCTC 9455的抗菌作用。一种分离细菌素Lacticin NK34酶的方法，包括： 使用菌株分离培养的步骤; 测定细菌素的抗菌活性的步骤; 研究乳酸NK34酶的特征的一个步骤; 以及测定对乳腺癌病原菌的抗菌作用的步骤。
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公开(公告)号：KR1020090093292A
公开(公告)日：2009-09-02
申请号：KR1020080018738
申请日：2008-02-29
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: A bacteriolysis extract of Saccharomyces chevalieri is provided to increase the activation of neutrophil and enhance non-specific defense activity against the pathogenic bacteria. An immune enhancer, vaccine adjuvant, adjuvant treating agent or feed additive comprises bacteriolysis extract of Saccharomyces chevalieri as an active ingredient. A method for manufacturing a bacteriolysis extract of Saccharomyces chevalieri comprises: a step of culturing Saccharomyces chevalieri in media at 26°C for 72 hours; a step of centrifuging media and suspending in PBS; a step of adding dissolution agent and treating at room temperature for 2-3 days; a step of confirming the dissolution status with a microscope; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a refrigerator.
Abstract translation: 提供雪花酵母的溶菌提取物以增加嗜中性粒细胞的活化并增强针对致病菌的非特异性防御活性。 免疫增强剂，疫苗佐剂，佐剂处理剂或饲料添加剂包括作为活性成分的雪花酵母的溶菌提取物。 酿酒酵母的溶菌提取物的制备方法包括：在培养基中在26℃下培养雪花酵母（Saccharomyces chevalieri）72小时的步骤; 离心培养基并悬浮于PBS中的步骤; 加入溶解剂并在室温下处理2-3天的步骤; 用显微镜确认溶出状态的步骤; 将pH浓度调节至6.5-7.2的步骤; 以及在冰箱中干燥和保持的步骤。
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公开(公告)号：KR1020090091884A
公开(公告)日：2009-08-31
申请号：KR1020080017089
申请日：2008-02-26
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: An immune enhancer and vaccine adjuvant additive for animal, which contains Zygosaccharomyces bailii lysates and an auxiliary therapeutic agent are provided to enhance the activation of neutrophil and non-specific protection effect to attack inoculation. An immune enhancer, vaccine adjuvant additive, auxiliary therapeutic agent and feed additive comprises a Zygosaccharomyces bailii lysates. A method for manufacturing the Zygosaccharomyces bailii lysate comprises: a step of culturing the Zygosaccharomyces bailii in the media at 26‹C for 72 hours; a step of centrifuging the cultured media and suspending in phosphate buffered saline (PBS); a step of adding dissolution agent and stirring at room temperature for two to three days; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a thermostatic chamber.
Abstract translation: 提供含有接合酵母发酵酵母裂解物和辅助治疗剂的动物免疫增强剂和疫苗佐剂添加剂，以增强中性粒细胞的活化和非特异性保护作用来攻击接种。 免疫增强剂，疫苗佐剂添加剂，辅助治疗剂和饲料添加剂包括接合酵母（Zygosaccharomyces bailii lysates）。 一种制造接合酵母发酵酵母裂解物的方法，包括：在培养基中培养接合酵母属酵母在26℃72小时的步骤; 将培养基离心并悬浮于磷酸盐缓冲盐水（PBS）中的步骤; 在室温下加入溶解剂并搅拌2〜3天的步骤; 将pH浓度调节至6.5-7.2的步骤; 以及在恒温室中干燥和保持的步骤。
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公开(公告)号：KR1020080068361A
公开(公告)日：2008-07-23
申请号：KR1020070006055
申请日：2007-01-19
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A plate agglutination tester and a method for measuring an immuno-reaction using the same are provided to decrease error of the result analysis in accordance with proficiency of a person performing diagnosis, show the degree of various immuno-reactions in an objective value and perform various statistical analyses, thereby being very useful for measuring the immuno-reaction. A plate agglutination tester consists of a transparent film and is characterized in that a section, which consists of a plurality of circles and where an antigen-antibody agglutination reaction occurs, is arranged and formed in horizontal and vertical directions on an upper side of the film. A method for measuring an immuno-reaction comprises the steps of: (a) mixing an antigen and an antibody specifically bound to the antigen with the plate agglutination tester to induce an agglutination reaction; (b) scanning the agglutination reaction induced plate agglutination tester and converting a scanning result into an image file; and (c) analyzing the obtained image file using a computer program capable of detecting fine agglutination reactants to numberize the intensity of the agglutination reaction. Further, the transparent film is an over head project film.
Abstract translation: 提供平板凝集试验机和使用其的免疫反应测定方法，根据进行诊断的人员的熟练程度来减少结果分析的误差，以目标值显示各种免疫反应的程度，并进行各种 统计分析，因此对测量免疫反应非常有用。 板凝集试验仪由透明膜构成，其特征在于，在膜的上侧，在水平和垂直方向上布置并形成由多个圆组成并且发生抗原 - 抗体凝集反应的部分 。 测定免疫反应的方法包括以下步骤：（a）将抗原与抗原特异性结合的抗体与平板凝集试验仪混合以诱导凝集反应; （b）扫描凝集反应诱导的板凝集测试仪并将扫描结果转换为图像文件; 和（c）使用能够检测细微凝集反应物的计算机程序来分析所获得的图像文件，以对凝集反应的强度进行编号。 此外，透明膜是顶部投影膜。
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Patent Agency Ranking
公开(公告)号：KR100430934B1
公开(公告)日：2004-05-14
申请号：KR1020010082976
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using recombinant 3ABC non-structural protein expressed in insect cells and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant baculovirus to be expressed in insect cells is prepared by co-transfection with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed by infection of the insect cells with the recombinant baculovirus. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the diluate on a plate; (2) reacting the testing serum with the plate; (3) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (4) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用在昆虫细胞和单克隆抗体中表达的重组3ABC非结构蛋白的口蹄疫诊断方法，从而比现有方法更快速和准确地诊断口蹄疫。 构成：编码来源于韩国口蹄疫病毒O / SKR / 2000的重组3ABC非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。在昆虫细胞中表达的重组杆状病毒由 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体共转染。通过用重组杆状病毒感染昆虫细胞表达重组3ABC非结构蛋白。 通过将在大肠杆菌中表达的重组3ABC非结构蛋白导入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系3F-11（KCTC 10138BP）。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 一种口蹄疫诊断方法，包括以下步骤：（1）将重组3ABC非结构蛋白稀释于包被缓冲液中，并将稀释液倒入平板中; （2）使测试血清与平板反应; （3）使与试验血清中的口蹄疫病毒抗体结合的缀合物与测试血清具有酶，放射性物质或荧光物质; 和（4）测量酶反应的强度，荧光反应或与缀合物的辐射反应。
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公开(公告)号：KR101201337B1
公开(公告)日：2012-11-15
申请号：KR1020100070745
申请日：2010-07-22
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 락토바실러스 존슨니(
Lactobacillus johnsonnii ) G22-2(KACC91458P), 락토바실러스 루테리(
Lactobacillus reuteri ) G8-5(KACC91450P) 및 락토바실러스 살리바리우스(
Lactobacillus salivarius ) G1-1(KACC91449P)로 이루어진 유산균 복합 균주를 유효성분으로 함유하는 사료첨가제에 관한 것이다.
본 발명의 유산균 복합 균주는 다양한 병원성 세균에 대한 항균력, 전분 분해능, 내산성, 내담즙성 및 장부착능이 우수하고 항생제 내성이 없어 안전하며 상호 억제 효과가 없어 사료첨가제로 사용하기에 적절하다. 본 발명의 유산균 복합 균주 사료첨가제는 여러 병원성 세균에 의한 감염으로부터 보호하고 사료의 이용 효율을 증대시킬 수 있을 뿐만 아니라 동물용 의약품을 대체할 수 있다.-
公开(公告)号：KR101098946B1
公开(公告)日：2011-12-28
申请号：KR1020090066955
申请日：2009-07-22
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 내산성, 내담즙, 및 장부착능이 있고, 한국농업미생물지원센터(KACC)에 기탁번호 KACC91449P로 기탁된 균주인 락토바실러스 살리바리우스(
Lactobacillus salivarius ) G1-1 및 이를 유효성분으로 함유한 사료첨가제 조성물에 관한 것이다.
본 발명에 따르면, 본 발명의 락토바실러스 살리바리우스 G1-1 균주는 다양한 병원성 세균에 대해 우수한 항균활성을 나타냄은 물론 내산성 및 내담즙성 등이 우수하므로, 장내 병원성 세균 억제제, 소화제, 정장제 등의 프로바이오틱 조성물로 제조하여 사료 및 동물용 의약품에 유용하게 활용될 수 있고, 축산 농가의 가축질병으로 인한 경제적 피해를 크게 감소시킬 수 있을 뿐만 아니라 수입 동물용 의약품을 대체할 수 있다.
락토바실러스 살리바리우스, 항균활성, 내담즙성, 내산성-
公开(公告)号：KR100968069B1
公开(公告)日：2010-07-08
申请号：KR1020070107293
申请日：2007-10-24
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 우 결핵균 항체에 특이적으로 반응하는 단백질 및 그 정제 방법에 관한 것으로, 더욱 상세하게는 우 결핵균 단백질 중 우결핵균 감염 혈청에 대한 항체 특이 단백질 및 그 정제 방법에 관한 것이다. 본 발명은 우결핵균의 혈청학적 진단 기술을 향상시킴으로써 우결핵의 확산방지를 도모할 수 있다.
우결핵, 단백질항원, 이온교환크로마토그래피, 항체검사, 면역블롯팅-
10.发明公开
公开(公告)号：KR1020030052858A
公开(公告)日：2003-06-27
申请号：KR1020010082976
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
CPC classification number: C12N15/866 , C07K14/005 , C07K16/1009 , C07K2317/14 , C12N5/163 , C12N2770/32131 , G01N33/56983 , G01N33/577 , G01N2333/09
Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using recombinant 3ABC non-structural protein expressed in insect cells and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant baculovirus to be expressed in insect cells is prepared by co-transfection with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed by infection of the insect cells with the recombinant baculovirus. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the diluate on a plate; (2) reacting the testing serum with the plate; (3) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (4) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用在昆虫细胞中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速，准确地诊断口蹄疫。 构成：编码来自韩国口蹄疫病毒O / SKR / 2000的重组体3ABC非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。在昆虫细胞中表达的重组杆状病毒通过 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体共转染。重组体3ABC非结构蛋白用重组杆状病毒感染昆虫细胞表达。 通过将在大肠杆菌中表达的重组3ABC非结构蛋白质导入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系3F-11（KCTC 10138BP）。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 口蹄疫的诊断方法包括以下步骤：（1）在包被缓冲溶液中稀释重组体3ABC非结构蛋白，将稀释液倒入平板上; （2）使测试血清与板反应; （3）使与测试血清中的口蹄疫病毒抗体结合并具有酶，放射性物质或荧光材料的缀合物与测试血清反应; 和（4）测量酶反应的强度，荧光反应或与缀合物的辐射反应。
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