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公开(公告)号：KR1019960008410B1
公开(公告)日：1996-06-26
申请号：KR1019930003655
申请日：1993-03-11
Applicant: 한국과학기술연구원
IPC: C12N15/52
Abstract: separating chromosome DNA from Corynebacterium glutamicum KCTC 1445; partly cutting it into constrained enzyme; obtaining gene library of the Corynebacterium glutamicum by adopting colitis germs as a host; and cloning into plasmid pCTS3(KCTC 85729) among the library. It is useful for amino acid fermentation.
Abstract translation: 从谷氨酸棒杆菌KCTC 1445分离染色体DNA; 部分切割成约束酶; 采用结肠炎细菌作为宿主获得谷氨酸棒杆菌的基因文库; 并在文库中克隆入质粒pCTS3（KCTC 85729）。 对氨基酸发酵有用。
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22.发明公开신균주 바실러스 속(Bacillus sp.) NS-70(KCTC 8555P)와 이를 이용한 신규한 단백질 가수분해효소의 제조방법 失效新菌株芽孢杆菌NS-70（KCTC 8555P）以及使用该菌株生产新型蛋白质水解酶的方法
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公开(公告)号：KR1019910007851B1
公开(公告)日：1991-10-02
申请号：KR1019890011660
申请日：1989-08-16
Applicant: 한국과학기술연구원
IPC: C12N1/20
Abstract: A method for culturing lactobacillus spp. TSC-66 comprises: (a) culturing lactobacillus spp. TSC-66 (KCTC 8447P) in the culture medium at 30 deg.C for 6-24hrs to obtain the starter; (b) innoculating the obtd. starter the medium, and culturing at 30-40 deg.C with shaking (60-250 rpm) and pouring into air or N2 gas (0.3-0.5 vvm) for 10-48hrs; (c) centrifuging or ultra filtrating the culture broth to obtain the cell. The cell, culture broth, etc is useful as fermenting agent for fermented milk, fermented cream, or pordn. of Kimchi, and additive for feed.
Abstract translation: 培养乳酸杆菌的方法 TSC-66包括：（a）培养乳酸杆菌属 TSC-66（KCTC 8447P）在培养基中30℃下培养6-24h，得到起子; （b）接受该通知。 起动培养基，并在30-40℃振荡（60-250rpm）下培养并倒入空气或N 2气（0.3-0.5vvm）中10-48小时; （c）离心或超滤培养液以获得细胞。 细胞，培养液等可用作发酵乳，发酵奶油或头发的发酵剂。 的泡菜和饲料添加剂。
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Patent Agency Ranking26.发明公开써모액티노마이세스속(THERMOACTINOMYCES SP.) E79의 신규한이화대사물 조절 단백질 A 및 이를 코딩하는 DNA 失效来自热休克霉素SP的新型CATABOLITE控制蛋白 E79和编码它的DNA
公开(公告)号：KR1020000025930A
公开(公告)日：2000-05-06
申请号：KR1019980043230
申请日：1998-10-15
Applicant: 한국과학기술연구원
IPC: C12N15/31
CPC classification number: C12N15/74 , A61K38/00 , C07K14/195
Abstract: PURPOSE: A novel catabolism control protein(CcpA) and DNA encoding the same are provided which induce a mutation discharging a catabolism repression phenotype, so it can be useful to construct the mutant which produces a hydrolysis enzyme regardless of a sugar composite ion in a fermentative medium. CONSTITUTION: Provided are a CcpA, DNA sequence encoding the same and a strong promoter thereof which is an inhibitory factor of enzyme production. DNA encoding the CcpA is described by SEQ ID NO. 1 and purified from Thermoactinomyces sp.. The CcpA is concerned in a catabolism repression as an inhibitory factor of enzyme production and regulates the synthesis of protein hydrolysis enzyme.
Abstract translation: 目的：提供一种新型的分解代谢控制蛋白（CcpA）和编码该DNA的DNA，其诱导排斥分解代谢阻遏表型的突变体，因此构建不产生糖复合离子的水解酶的突变体是有用的 中。 构成：提供了编码该酶的CcpA，DNA序列和作为酶生产的抑制因子的强启动子。 编码CcpA的DNA由SEQ ID NO： 1并从Thermoactinomyces sp。中纯化.CcpA涉及分解代谢抑制作为酶生产的抑制因子并调节蛋白质水解酶的合成。
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27.发明授权재조합 내열성 베타-타이로시네이즈를 이용하여 고농도 페놀함유 산업용 폐수로부터 페놀을 유용 아미노산으로 전환하여 제거하는 방법 失效通过使用重组耐热β-酪氨酸酶从含有高浓度苯酚的工业废水中将苯酚转化为有用的氨基酸的方法
公开(公告)号：KR100171159B1
公开(公告)日：1999-02-01
申请号：KR1019950020071
申请日：1995-07-08
Applicant: 한국과학기술연구원
Abstract: 본 발명은 고온균 유래의 내열성 β-타이로시네이즈 유전자를 포함하는 발현벡터로 형질전환된 재조합 대장균 JM105/pHLT1(KCTC 0158BP)을 배양하여 수득한 내열성 β-타이로시네이즈를 이용하여 페놀 함유 폐수 중의 페놀을 타이로신으로 전환시켜 침전시키는 단계를 포함하는, 페놀 함유 폐수로부터 페놀을 제거하는 방법에 관한 것으로, 본 발명의 방법에 따르면 10,000ppm 이상의 고농도의 페놀을 함유한 폐수 중의 페놀 농도를 1,000ppm 이하로 저하시킬 수 있다.
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29.发明授权신균주 슈우도모나스 속(Pseudomonas sp.) NS-83(KCTC 8600P)와 이를 이용한 신규한 단백질 가수분해효소의 제조방법 失效NOVEL PSEUDOMONAS SP。 和新蛋白的生产方法
公开(公告)号：KR100127100B1
公开(公告)日：1997-12-29
申请号：KR1019940010758
申请日：1994-05-17
Applicant: 한국과학기술연구원
IPC: C12N1/20
Abstract: PSEUDOMONAS SP. NS-83(KCTC 8600P) AND production of novel protein hydrolysis enzyme is disclosed in this invention. PSEUDOMONAS SP. NS-83(KCTC 8600P) is injected in PH6-8 of medium made by polypepton, extract of enzyme, extract of cow meat, glycol, KH2PO4, K2HPO4, MgSO4.7H2O and NaCl, cultivated for 6 hours at 35-40 degree Celsius. Thereafter, above-cultivated liquid is fermented in medium made by aqueous starch, phytonpepton, KH2PO4, K2HPO4, MgSO4.7H2O and NaCl, for 15 hours at 50 degree Celsius to produce protein hydrolysis enzyme having characteristics below; molecular weight : 32,000 dalton, optimum pH : 8.0, optimum temperature : 60deg.C.
Abstract translation: PSEUDOMONAS SP。 NS-83（KCTC 8600P）和新型蛋白质水解酶的生产在本发明中公开。 PSEUDOMONAS SP。 将NS-83（KCTC 8600P）注射到由多肽，酶提取物，牛肉提取物，二醇，KH 2 PO 4，K 2 HPO 4，MgSO 4·7H 2 O和NaCl制成的培养基的PH6-8中，在35-40摄氏度下培养6小时 。 此后，将上述培养液体在含水淀粉，植物磷酸盐，KH 2 PO 4，K 2 HPO 4，MgSO 4·7H 2 O和NaCl的培养基中在50℃下发酵15小时以产生具有以下特征的蛋白质水解酶; 分子量：32,000道尔顿，最适pH：8.0，最适温度：60℃。
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30.发明授权티로신 페놀리아제(Tyrosine phenollyase)를 코드(code)하는 유전자, 상기 유전자에 의해 형질전환된 대장균 및 이를 이용한 티로신 페놀리아제의 제조방법 失效编码酪氨酸苯酚酶的基因，用该基因转化的大肠杆菌，以及使用其的酪氨酸苯酚酶的生产方法
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