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31.发明公开
公开(公告)号：KR1020140000810A
公开(公告)日：2014-01-06
申请号：KR1020120068128
申请日：2012-06-25
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12Q1/68 , G01N33/569 , C12Q1/04
CPC classification number: G01N33/5695 , C12Q1/04 , C12Q1/686
Abstract: The present invention relates to a detection method using magnetic nanoparticles for rapidly isolating Mycobacterium avium subsp. paratuberculosis from fecal samples and, more specifically, to a method for detecting the Mycobacterium avium subsp. paratuberculosis which isolates the Mycobacterium avium subsp. paratuberculosis with high sensitivity which can be existed in animal fecal samples using the magnetic nanoparticles to which Mycobacterium avium subsp. paratuberculosis specific antibodies are combined and rapidly and simply checks infection of the Mycobacterium avium subsp. paratuberculosis by performing polymerase chain reaction using primers which are specifically combined to DNA of the Mycobacterium avium subsp. paratuberculosis. According to the present invention, the detection method using the magnetic nanoparticles for rapidly isolating the Mycobacterium avium subsp. paratuberculosis from fecal samples can easily detect the Mycobacterium avium subsp. paratuberculosis even if small number of the Mycobacterium avium subsp. paratuberculosis is existed in biological samples such as feces and prevent contagion to different entities by isolating the infected entities from an allied army by performing the method simply and rapidly.
Abstract translation: 本发明涉及使用磁性纳米颗粒快速分离鸟分枝杆菌亚种的检测方法。 来自粪便样品的副结核病，更具体地，涉及用于检测鸟分枝杆菌亚种的方法。 分离鸟分枝杆菌亚种的副结核病 具有高灵敏度的副结核病，其可以存在于动物粪便样品中，使用鸟分枝杆菌亚种的磁性纳米颗粒。 组合结核分枝杆菌特异性抗体并快速简单地检查鸟分枝杆菌感染。 使用与鸟分枝杆菌亚种DNA的特异性组合的引物进行聚合酶链反应，来治疗副结核病。 副结核病。 根据本发明，使用磁性纳米颗粒快速分离鸟分枝杆菌亚种的检测方法。 来自粪便样本的副结核病可以很容易地检测到鸟分枝杆菌。 副结核病即使少数分枝杆菌分枝杆菌。 副结核病存在于粪便等生物样本中，通过简单，快速地进行方法，将受感染的实体与盟军分离，防止对不同实体的传染。
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32.发明公开중합효소연쇄반응을 이용한 생물학적 시료내에서 퀴놀론 내성 캠필로박터균을 검출하는 방법 및 이에 사용되는 키트 有权使用聚合酶链反应从生物样品检测和鉴定喹诺酮抗性球蛋白的方法和用于该方法的试剂盒
公开(公告)号：KR1020130054771A
公开(公告)日：2013-05-27
申请号：KR1020110120356
申请日：2011-11-17
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 솔젠트 (주)
CPC classification number: Y02A50/451 , C12Q1/686 , C12Q1/04
Abstract: PURPOSE: A method for detecting a quinolone resistant Campylobacter variant in a biological sample and a primer set and a kit for PCR for the same are provided to cheaply and simply detect and distinguish quinolone resistant variants of C. jejuni and C.coli. CONSTITUTION: A method for distinguishing and detecting a wild type and a quinolone resistant variant type of C. jejuni and C.coli in a biological sample comprises: a step of isolating DNA from the biological sample; a step of performing PCR using the DNA as a template and a primer set for PCR containing an oligonucleotide of sequence numbers 1, 2, 3, 4, 5, and 6; and a step of analyzing a PCR product and distinguishing wild type and variant type.
Abstract translation: 目的：提供一种用于检测生物样品中的喹诺酮抗性弯曲杆菌变异体的方法，以及引物组和用于PCR的PCR试剂盒，以廉价且简单地检测和区分空肠弯曲杆菌和C.coli的喹诺酮抗性变体。 构成：在生物样品中鉴别和检测野生型和喹诺酮抗性变体型空肠弯曲杆菌和C.coli的方法包括：从生物样品中分离DNA的步骤; 使用DNA作为模板进行PCR的步骤和含有序列号1,2,3,4,5和6的寡核苷酸的PCR引物组; 以及分析PCR产物并区分野生型和变异型的步骤。
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公开(公告)号：KR101144994B1
公开(公告)日：2012-06-27
申请号：KR1020080041975
申请日：2008-05-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/002 , A61K39/00
Abstract: PURPOSE: A vaccine of neosporosis and a method for producing neosporosis are provided to induce infection of low level and prevent neosporosis. CONSTITUTION: A vaccine for bovine neosporosis contains Neospora caninum antigen. The vaccine further comprises Polygen^TM, IMS1313^TM, or PBS^TM as an adjuvant. A method for producing the vaccine comprises: a step of culturing Neospora protozoa in a cell; a step of collecting cultured Neospora protozoa; and a step of treating the Neospora protozoa with antigen.
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公开(公告)号：KR1020120024290A
公开(公告)日：2012-03-14
申请号：KR1020100087127
申请日：2010-09-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: G01N33/544 , G01N33/569
Abstract: PURPOSE: A method for binding Leishmania infantum antigen to latex beads is provided to enable simple and cheap diagnosis without expensive equipment. CONSTITUTION: A method for binding Leishmania infantum(ATCC 50134) antigen to latex beads comprises: a step of the Leishmania infantum antigen in 45mM~55mM HEPES(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and 145-155mM NaCl buffer in a concentration of 1.45mg/ml-1.55mg/ml; a step of reacting the latex beads into the diluted antigen at 35-39°C for 0.5-1.5 hours and then reacting at 3-5°C for 8-12 hours; a step of centrifuging the reacted antigen at 5,000rpm-7000rpm for 15-25 minutes; and a step of treating 10% BSA(Bovine Albumin Serum) for 2.5-3.5 hours.
Abstract translation: 目的：提供一种将利什曼原虫婴幼儿抗原结合到乳胶珠上的方法，以便在没有昂贵的设备的情况下进行简单而便宜的诊断。 构成：将利什曼原虫（ATCC 50134）抗原结合到乳胶珠的方法包括：在45mM〜55mM HEPES（4-（2-羟乙基）-1-哌嗪乙磺酸）和145-155mM NaCl缓冲液中的利什曼原虫婴幼儿抗原的步骤 浓度为1.45mg / ml-1.55mg / ml; 将乳胶珠在35-39℃下反应0.5-1.5小时，然后在3-5℃下反应8-12小时; 以5000rpm-7000rpm离心反应的抗原15-25分钟的步骤; 以及将10％BSA（牛白蛋白血清）处理2.5-3.5小时的步骤。
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公开(公告)号：KR1020110052295A
公开(公告)日：2011-05-18
申请号：KR1020090109275
申请日：2009-11-12
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A method for diagnosing cattle tuberculosis using intradermal diagnostic composition is provided to easily prepare a large amount of intradermal diagnostic composition and to ensure diagnosis accuracy. CONSTITUTION: An intradermal diagnostic composition for testing cattle tuberculosis contains recombinant proteins of Esat6, HspX, PhoS, MPB70, MPB64, MPB83, Ag85, and SahH. A method for diagnosing tuberculosis of mammal except for human is performed using the intradermal diagnostic composition containing the recombinant proteins. The recombinant protein is prepared by isolating from Mycobacterium bovis and performing PCR.
Abstract translation: 目的：提供使用皮内诊断组合物诊断牛结核病的方法，以容易地制备大量皮内诊断组合物并确保诊断准确性。 构成：用于测试牛结核的皮内诊断组合物含有Esat6，HspX，PhoS，MPB70，MPB64，MPB83，Ag85和SahH的重组蛋白。 使用含有重组蛋白质的皮内诊断组合物进行用于诊断除人以外的哺乳动物结核病的方法。 通过从牛分枝杆菌中分离并进行PCR来制备重组蛋白。
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Patent Agency Ranking
公开(公告)号：KR1020090117448A
公开(公告)日：2009-11-12
申请号：KR1020080043496
申请日：2008-05-09
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A23K10/30 , A23K20/195
Abstract: PURPOSE: An antioxidant for supplementing feed using Forsythiae fructus extract and a preparation method for the Forsythiae Fructus extract are provided to show improved daily gain, feed intake, and feed efficiency compared to a control group. CONSTITUTION: An antioxidant for supplementing feed contains Forsythiae fructus extract. A method for preparing Forsythiae fructus extract comprises the following steps of: washing 100g Forsythiae fructus with water to remove dust; placing the washed Forsythiae fructus in a white bag; adding 1,000ml distilled water into the white bag to soak for 20-30 minutes; boiling down the soaked Forsythiae fructus for 150 minutes and obtaining an extract; adding another 800ml distilled water and boiling down for 150 minutes to obtain the extract; adding another 700ml distilled water and boiling down for 150 minutes to obtain the extract; collecting the extracts and concentrating until the volume becomes 100ml; and freeze-drying the concentrate.
Abstract translation: 目的：提供使用连翘果提取物补充饲料的抗氧化剂和连翘果提取物的制备方法，以显示与对照组相比改善的日增重，进食量和饲料效率。 构成：补充饲料的抗氧化剂含有连翘果提取物。 连翘果提取物的制备方法为：用水洗涤连翘100份，除去灰尘; 将洗涤的连翘属植物置于白色袋中; 在白色袋中加入1000ml蒸馏水浸泡20-30分钟; 煮熟连翘150分钟，得到提取物; 加入800ml蒸馏水，煮沸150分钟，得到提取物; 再加入700ml蒸馏水，煮沸150分钟，得到提取物; 收集提取物并浓缩直到体积变成100ml; 并冷冻干燥浓缩物。
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公开(公告)号：KR1020090093292A
公开(公告)日：2009-09-02
申请号：KR1020080018738
申请日：2008-02-29
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: A bacteriolysis extract of Saccharomyces chevalieri is provided to increase the activation of neutrophil and enhance non-specific defense activity against the pathogenic bacteria. An immune enhancer, vaccine adjuvant, adjuvant treating agent or feed additive comprises bacteriolysis extract of Saccharomyces chevalieri as an active ingredient. A method for manufacturing a bacteriolysis extract of Saccharomyces chevalieri comprises: a step of culturing Saccharomyces chevalieri in media at 26°C for 72 hours; a step of centrifuging media and suspending in PBS; a step of adding dissolution agent and treating at room temperature for 2-3 days; a step of confirming the dissolution status with a microscope; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a refrigerator.
Abstract translation: 提供雪花酵母的溶菌提取物以增加嗜中性粒细胞的活化并增强针对致病菌的非特异性防御活性。 免疫增强剂，疫苗佐剂，佐剂处理剂或饲料添加剂包括作为活性成分的雪花酵母的溶菌提取物。 酿酒酵母的溶菌提取物的制备方法包括：在培养基中在26℃下培养雪花酵母（Saccharomyces chevalieri）72小时的步骤; 离心培养基并悬浮于PBS中的步骤; 加入溶解剂并在室温下处理2-3天的步骤; 用显微镜确认溶出状态的步骤; 将pH浓度调节至6.5-7.2的步骤; 以及在冰箱中干燥和保持的步骤。
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公开(公告)号：KR1020090091884A
公开(公告)日：2009-08-31
申请号：KR1020080017089
申请日：2008-02-26
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: An immune enhancer and vaccine adjuvant additive for animal, which contains Zygosaccharomyces bailii lysates and an auxiliary therapeutic agent are provided to enhance the activation of neutrophil and non-specific protection effect to attack inoculation. An immune enhancer, vaccine adjuvant additive, auxiliary therapeutic agent and feed additive comprises a Zygosaccharomyces bailii lysates. A method for manufacturing the Zygosaccharomyces bailii lysate comprises: a step of culturing the Zygosaccharomyces bailii in the media at 26‹C for 72 hours; a step of centrifuging the cultured media and suspending in phosphate buffered saline (PBS); a step of adding dissolution agent and stirring at room temperature for two to three days; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a thermostatic chamber.
Abstract translation: 提供含有接合酵母发酵酵母裂解物和辅助治疗剂的动物免疫增强剂和疫苗佐剂添加剂，以增强中性粒细胞的活化和非特异性保护作用来攻击接种。 免疫增强剂，疫苗佐剂添加剂，辅助治疗剂和饲料添加剂包括接合酵母（Zygosaccharomyces bailii lysates）。 一种制造接合酵母发酵酵母裂解物的方法，包括：在培养基中培养接合酵母属酵母在26℃72小时的步骤; 将培养基离心并悬浮于磷酸盐缓冲盐水（PBS）中的步骤; 在室温下加入溶解剂并搅拌2〜3天的步骤; 将pH浓度调节至6.5-7.2的步骤; 以及在恒温室中干燥和保持的步骤。
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39.发明公开동물의 면역능을 증강시키는 노란 민들레 열탕 추출물, 그제법 및 용도 无效用于改善动物免疫力的UM UM AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME
公开(公告)号：KR1020090021611A
公开(公告)日：2009-03-04
申请号：KR1020070086251
申请日：2007-08-27
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K36/288 , A61P37/02
Abstract: Provided is an immunostimulant using dandelion extract which improves a function of a nonspecific defense against pathogen and increases activity of animal immunocytes such as neutrophil and macrophages. An immunostimulant using dandelion extract comprises dandelion extract obtained by a hot water process as an active ingredient. A method for preparing the immunostimulant comprises a steps of extracting the dandelion extract at a temperature of 90~100°C by hot distilled water. The dandelion extract can be used independently or with additives which are generally used.
Abstract translation: 提供使用蒲公英提取物的免疫刺激剂，其改善对病原体的非特异性防御的功能并增加动物免疫细胞如嗜中性粒细胞和巨噬细胞的活性。 使用蒲公英提取物的免疫刺激剂包含通过热水法获得的蒲公英提取物作为活性成分。 制备免疫刺激剂的方法包括以下步骤：通过热蒸馏水在90〜100℃的温度下提取蒲公英提取物。 蒲公英提取物可以单独使用或与通常使用的添加剂一起使用。
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公开(公告)号：KR1020080081499A
公开(公告)日：2008-09-10
申请号：KR1020070021570
申请日：2007-03-05
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/064 , C12N1/18
Abstract: An exo-polysaccharide derived from a Saccharomyces spp. strain is provided to enhance various activities of neutrophil, macrophage and lymphocyte, thereby being widely applied to an animal immunity-enhancing agent, a vaccine supplemental additive and an adjuvant therapeutic agent. An animal immunity enhancing agent comprises exo-polysaccharides extracted from a culture material of a Saccharomyces spp. strain. A method for preparing the exo-polysaccharides derived from the Saccharomyces spp. strain comprises the steps of: (a) after suspending pure colonies obtained by culturing the Saccharomyces spp. strain in sterile saline solution, inoculating 1 ml of the suspension into 1,000 ml of yeast malt broth and culturing it at a temperature of 28 deg.C for 72 hours; (b) after adding two times amount of distilled water into a culture material obtained from the step(a) and mixing it, boiling the mixture at a temperature of 100 deg.C for 1 hour and centrifuging the boiled mixture under 5,000xg for 30 minutes to collect supernatant therefrom; and (c) after adding two times amount of ethyl alcohol to the supernatant and mixing it, leaving a mixture in a cool chamber at a temperature of 4 deg.C for 2 days and collecting precipitated polysaccharides therefrom.
Abstract translation: 衍生自酵母属（Saccharomyces spp。）的外多糖。 提供菌株以增强嗜中性粒细胞，巨噬细胞和淋巴细胞的各种活性，从而广泛应用于动物免疫增强剂，疫苗补充添加剂和辅助治疗剂。 动物免疫增强剂包括从酵母属的培养物质中提取的外多糖。 应变。 一种制备衍生自酵母属（Saccharomyces spp）的外多糖的方法。 菌株包括以下步骤：（a）悬浮通过培养酵母属（Saccharomyces spp）获得的纯菌落后。 在无菌生理盐水溶液中，将1ml悬浮液接种到1,000ml酵母麦芽培养液中，并在28℃的温度下培养72小时; （b）在向步骤（a）获得的培养物中加入两倍量的蒸馏水并混合后，在100℃的温度下将混合物煮沸1小时，并将煮沸的混合物在5,000xg下离心30分钟 分钟收集上清液; 和（c）在向上清液中加入两倍量的乙醇并混合后，在4℃的温度下在冷室中留下混合物2天，并从中收集沉淀的多糖。
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