Abstract:
The present invention relates to a detection method using magnetic nanoparticles for rapidly isolating Mycobacterium avium subsp. paratuberculosis from fecal samples and, more specifically, to a method for detecting the Mycobacterium avium subsp. paratuberculosis which isolates the Mycobacterium avium subsp. paratuberculosis with high sensitivity which can be existed in animal fecal samples using the magnetic nanoparticles to which Mycobacterium avium subsp. paratuberculosis specific antibodies are combined and rapidly and simply checks infection of the Mycobacterium avium subsp. paratuberculosis by performing polymerase chain reaction using primers which are specifically combined to DNA of the Mycobacterium avium subsp. paratuberculosis. According to the present invention, the detection method using the magnetic nanoparticles for rapidly isolating the Mycobacterium avium subsp. paratuberculosis from fecal samples can easily detect the Mycobacterium avium subsp. paratuberculosis even if small number of the Mycobacterium avium subsp. paratuberculosis is existed in biological samples such as feces and prevent contagion to different entities by isolating the infected entities from an allied army by performing the method simply and rapidly.
Abstract:
PURPOSE: A method for detecting a quinolone resistant Campylobacter variant in a biological sample and a primer set and a kit for PCR for the same are provided to cheaply and simply detect and distinguish quinolone resistant variants of C. jejuni and C.coli. CONSTITUTION: A method for distinguishing and detecting a wild type and a quinolone resistant variant type of C. jejuni and C.coli in a biological sample comprises: a step of isolating DNA from the biological sample; a step of performing PCR using the DNA as a template and a primer set for PCR containing an oligonucleotide of sequence numbers 1, 2, 3, 4, 5, and 6; and a step of analyzing a PCR product and distinguishing wild type and variant type.
Abstract:
PURPOSE: A vaccine of neosporosis and a method for producing neosporosis are provided to induce infection of low level and prevent neosporosis. CONSTITUTION: A vaccine for bovine neosporosis contains Neospora caninum antigen. The vaccine further comprises Polygen^TM, IMS1313^TM, or PBS^TM as an adjuvant. A method for producing the vaccine comprises: a step of culturing Neospora protozoa in a cell; a step of collecting cultured Neospora protozoa; and a step of treating the Neospora protozoa with antigen.
Abstract:
PURPOSE: A method for binding Leishmania infantum antigen to latex beads is provided to enable simple and cheap diagnosis without expensive equipment. CONSTITUTION: A method for binding Leishmania infantum(ATCC 50134) antigen to latex beads comprises: a step of the Leishmania infantum antigen in 45mM~55mM HEPES(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and 145-155mM NaCl buffer in a concentration of 1.45mg/ml-1.55mg/ml; a step of reacting the latex beads into the diluted antigen at 35-39°C for 0.5-1.5 hours and then reacting at 3-5°C for 8-12 hours; a step of centrifuging the reacted antigen at 5,000rpm-7000rpm for 15-25 minutes; and a step of treating 10% BSA(Bovine Albumin Serum) for 2.5-3.5 hours.
Abstract:
PURPOSE: A method for diagnosing cattle tuberculosis using intradermal diagnostic composition is provided to easily prepare a large amount of intradermal diagnostic composition and to ensure diagnosis accuracy. CONSTITUTION: An intradermal diagnostic composition for testing cattle tuberculosis contains recombinant proteins of Esat6, HspX, PhoS, MPB70, MPB64, MPB83, Ag85, and SahH. A method for diagnosing tuberculosis of mammal except for human is performed using the intradermal diagnostic composition containing the recombinant proteins. The recombinant protein is prepared by isolating from Mycobacterium bovis and performing PCR.
Abstract:
PURPOSE: An antioxidant for supplementing feed using Forsythiae fructus extract and a preparation method for the Forsythiae Fructus extract are provided to show improved daily gain, feed intake, and feed efficiency compared to a control group. CONSTITUTION: An antioxidant for supplementing feed contains Forsythiae fructus extract. A method for preparing Forsythiae fructus extract comprises the following steps of: washing 100g Forsythiae fructus with water to remove dust; placing the washed Forsythiae fructus in a white bag; adding 1,000ml distilled water into the white bag to soak for 20-30 minutes; boiling down the soaked Forsythiae fructus for 150 minutes and obtaining an extract; adding another 800ml distilled water and boiling down for 150 minutes to obtain the extract; adding another 700ml distilled water and boiling down for 150 minutes to obtain the extract; collecting the extracts and concentrating until the volume becomes 100ml; and freeze-drying the concentrate.
Abstract:
A bacteriolysis extract of Saccharomyces chevalieri is provided to increase the activation of neutrophil and enhance non-specific defense activity against the pathogenic bacteria. An immune enhancer, vaccine adjuvant, adjuvant treating agent or feed additive comprises bacteriolysis extract of Saccharomyces chevalieri as an active ingredient. A method for manufacturing a bacteriolysis extract of Saccharomyces chevalieri comprises: a step of culturing Saccharomyces chevalieri in media at 26°C for 72 hours; a step of centrifuging media and suspending in PBS; a step of adding dissolution agent and treating at room temperature for 2-3 days; a step of confirming the dissolution status with a microscope; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a refrigerator.
Abstract:
An immune enhancer and vaccine adjuvant additive for animal, which contains Zygosaccharomyces bailii lysates and an auxiliary therapeutic agent are provided to enhance the activation of neutrophil and non-specific protection effect to attack inoculation. An immune enhancer, vaccine adjuvant additive, auxiliary therapeutic agent and feed additive comprises a Zygosaccharomyces bailii lysates. A method for manufacturing the Zygosaccharomyces bailii lysate comprises: a step of culturing the Zygosaccharomyces bailii in the media at 26‹C for 72 hours; a step of centrifuging the cultured media and suspending in phosphate buffered saline (PBS); a step of adding dissolution agent and stirring at room temperature for two to three days; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a thermostatic chamber.
Abstract:
Provided is an immunostimulant using dandelion extract which improves a function of a nonspecific defense against pathogen and increases activity of animal immunocytes such as neutrophil and macrophages. An immunostimulant using dandelion extract comprises dandelion extract obtained by a hot water process as an active ingredient. A method for preparing the immunostimulant comprises a steps of extracting the dandelion extract at a temperature of 90~100°C by hot distilled water. The dandelion extract can be used independently or with additives which are generally used.
Abstract:
An exo-polysaccharide derived from a Saccharomyces spp. strain is provided to enhance various activities of neutrophil, macrophage and lymphocyte, thereby being widely applied to an animal immunity-enhancing agent, a vaccine supplemental additive and an adjuvant therapeutic agent. An animal immunity enhancing agent comprises exo-polysaccharides extracted from a culture material of a Saccharomyces spp. strain. A method for preparing the exo-polysaccharides derived from the Saccharomyces spp. strain comprises the steps of: (a) after suspending pure colonies obtained by culturing the Saccharomyces spp. strain in sterile saline solution, inoculating 1 ml of the suspension into 1,000 ml of yeast malt broth and culturing it at a temperature of 28 deg.C for 72 hours; (b) after adding two times amount of distilled water into a culture material obtained from the step(a) and mixing it, boiling the mixture at a temperature of 100 deg.C for 1 hour and centrifuging the boiled mixture under 5,000xg for 30 minutes to collect supernatant therefrom; and (c) after adding two times amount of ethyl alcohol to the supernatant and mixing it, leaving a mixture in a cool chamber at a temperature of 4 deg.C for 2 days and collecting precipitated polysaccharides therefrom.