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    ＨＩＶ-1 ｇｐ41의 Ｎ-말단에 특이적으로 결합하는 항체
    11.
    发明授权
    ＨＩＶ-1 ｇｐ41의 Ｎ-말단에 특이적으로 결합하는 항체 失效
    HIV-1 gp41的N-末端核苷酸序列可用于检测抗体

    公开(公告)号：KR100464926B1

    公开(公告)日：2005-01-05

    申请号：KR1020010056800

    申请日：2001-09-14

    Applicant: 한국과학기술연구원

    Inventor： 유재훈 ,  유연규 ,  김기선 ,  최소영

    IPC: C07K16/18

    Abstract: PURPOSE: Antibodies specifically binding to the N-terminal of HIV gp 41 are provided, thereby function of gp41 which is essential for the infection of HIV can be inhibited, so that it can be useful for prevention and treatment of HIV. CONSTITUTION: The antibodies which are specifically binding to the N-terminal of HIV gp 41, are capable of recognizing gp 41-ex(extracellular domain), and inhibit function of gp 41 are provided, wherein the heavy chain of an antibody has the amino acid sequences of SEQ ID NO: 1 and the light chain of the antibody has the amino acid sequence of SEQ ID NO: 2; the heavy chain of an antibody has the amino acid sequences of SEQ ID NO: 3 and the light chain of the antibody has the amino acid sequence of SEQ ID NO: 4; the heavy chain of an antibody has the amino acid sequences of SEQ ID NO: 5 and the light chain of the antibody has the amino acid sequence of SEQ ID NO: 6; and the heavy chain of an antibody has the amino acid sequences of SEQ ID NO: 7 and the light chain of the antibody has the amino acid sequence of SEQ ID NO: 8.

    Abstract translation: 目的：提供特异性结合于HIV gp41 N末端的抗体，从而可以抑制对HIV感染至关重要的gp41的功能，从而可用于HIV的预防和治疗。 构成：特异性结合于HIV gp 41 N末端的抗体能够识别gp 41-ex（胞外结构域），并且提供gp 41的抑制功能，其中抗体的重链具有氨基酸 SEQ ID NO：1的氨基酸序列和抗体的轻链具有SEQ ID NO：2的氨基酸序列; 抗体的重链具有SEQ ID NO：3的氨基酸序列，抗体的轻链具有SEQ ID NO：4的氨基酸序列; 抗体的重链具有SEQ ID NO：5的氨基酸序列，抗体的轻链具有SEQ ID NO：6的氨基酸序列; 并且抗体的重链具有SEQ ID NO：7的氨基酸序列，并且抗体的轻链具有SEQ ID NO：8的氨基酸序列。

    ＨＩＶ의 감염 억제 펩타이드
    12.
    发明授权
    ＨＩＶ의 감염 억제 펩타이드 失效
    HIV의감염억제펩타이드

    公开(公告)号：KR100447943B1

    公开(公告)日：2004-09-08

    申请号：KR1020000065664

    申请日：2000-11-06

    Applicant: 한국과학기술연구원

    Inventor： 유연규 ,  김기선 ,  진봉석

    IPC: A61K38/03

    CPC classification number: A61K38/10

    Abstract: The present invention relates to peptides that can inhibit the infection of HIV, and more particularly, to peptides consisting of less than 30 amino acids which can introduce a helix capping motif into a peptide derived from C-terminal helical region (its 628-646th amino acid region) of gp41, an envelope glycoprotein of HIV, as well as consisting of the symmetrical bivalent peptide through the introduction of a branched amino acid, Fmoc-Lys(Fmoc)-OH at C-terminus of its peptide, and induce a more stable helical structure thus inhibiting the infection of HIV.

    Abstract translation: 本发明涉及能够抑制HIV感染的肽，更具体地涉及由少于30个氨基酸组成的肽，其可以将螺旋帽基序引入衍生自C端螺旋区（其第628-646位氨基酸 通过在其肽的C-末端引入支链氨基酸Fmoc-Lys（Fmoc）-OH，并诱导更多的gp41（一种HIV的包膜糖蛋白），以及由对称二价肽组成 稳定的螺旋结构从而抑制HIV的感染。

    단백질의 열안정화 방법
    13.
    发明授权
    단백질의 열안정화 방법 失效
    단백질의열안정화방법

    公开(公告)号：KR100390769B1

    公开(公告)日：2003-07-10

    申请号：KR1020000063596

    申请日：2000-10-27

    Applicant: 한국과학기술연구원

    Inventor： 김기선 ,  이덕연

    IPC: C12N15/00

    CPC classification number: C12N9/0036 ,  C07K14/195 ,  C07K14/245 ,  C12P21/02

    Abstract: 본 발명은 단백질의 열안정화 방법에 관한 것으로서, 더욱 상세하게는 수용성 단백질의 표면에 있는 친수성 아미노산 잔기인 아스파르트산(aspartic acid)을 특이자리돌연변이(site-specific mutagenesis) 기법을 이용하여 글루탐산 (glutamic acid)으로 치환함으로써 열안정성을 높이는 단백질의 열안정화 방법에 관한 것이다. 따라서, 이러한 본 발명에 따르면 서로 유사한 정전기적 형질과 분자량을 지닌 친수성 아미노산이며 대부분 단백질의 표면에 존재하는 아스파르트산을 글루탐산으로 치환함으로써 단백질의 구조가 규명되어 있지 않은 상태에서도 단백질의 구조나 기능에 영향을 주지 않은 채 열안정성을 높일 수 있어 의료, 식품, 화학 등 단백질을 이용하는 각종 산업에 응용할 수 있는 효과가 있다.

    Abstract translation: 本发明涉及通过位点特异性诱变用谷氨酸取代存在于水溶性蛋白质表面的亲水性氨基酸的天冬氨酸，提高蛋白质的热稳定性的方法。 存在于大多数蛋白质上的天冬氨酸（亲水性氨基酸）与谷氨酸（其在静电特征和分子量两者中与天冬氨酸非常相似）可以提高所得蛋白质的热稳定性而不影响其结构或功能 从而能够将其应用扩展到各种工业领域，例如医药，食品和化学。

    ＨＩＶ-1 ｇｐ41의 Ｎ-말단에 특이적으로 결합하는 항체
    14.
    发明公开
    ＨＩＶ-1 ｇｐ41의 Ｎ-말단에 특이적으로 결합하는 항체 失效
    抗体特异性绑定到HIV终端GP 41

    公开(公告)号：KR1020030024004A

    公开(公告)日：2003-03-26

    申请号：KR1020010056800

    申请日：2001-09-14

    Applicant: 한국과학기술연구원

    Inventor： 유재훈 ,  유연규 ,  김기선 ,  최소영

    IPC: C07K16/18

    CPC classification number: C07K16/1045 ,  A61K39/21

    Abstract: PURPOSE: Antibodies specifically binding to the N-terminal of HIV gp 41 are provided, thereby function of gp41 which is essential for the infection of HIV can be inhibited, so that it can be useful for prevention and treatment of HIV. CONSTITUTION: The antibodies which are specifically binding to the N-terminal of HIV gp 41, are capable of recognizing gp 41-ex(extracellular domain), and inhibit function of gp 41 are provided, wherein the heavy chain of an antibody has the amino acid sequences of SEQ ID NO: 1 and the light chain of the antibody has the amino acid sequence of SEQ ID NO: 2; the heavy chain of an antibody has the amino acid sequences of SEQ ID NO: 3 and the light chain of the antibody has the amino acid sequence of SEQ ID NO: 4; the heavy chain of an antibody has the amino acid sequences of SEQ ID NO: 5 and the light chain of the antibody has the amino acid sequence of SEQ ID NO: 6; and the heavy chain of an antibody has the amino acid sequences of SEQ ID NO: 7 and the light chain of the antibody has the amino acid sequence of SEQ ID NO: 8.

    Abstract translation: 目的：提供与HIV gp 41的N-末端特异性结合的抗体，因此可以抑制对HIV感染必不可少的gp41的功能，因此可用于预防和治疗HIV。 构成：与HIV gp41的N-末端特异性结合的抗体能够识别gp 41-ex（细胞外结构域），并提供抑制gp41功能，其中抗体的重链具有氨基 SEQ ID NO：1的酸序列和抗体的轻链具有SEQ ID NO：2的氨基酸序列; 抗体的重链具有SEQ ID NO：3的氨基酸序列，抗体的轻链具有SEQ ID NO：4的氨基酸序列; 抗体的重链具有SEQ ID NO：5的氨基酸序列，抗体的轻链具有SEQ ID NO：6的氨基酸序列; 抗体的重链具有SEQ ID NO：7的氨基酸序列，抗体的轻链具有SEQ ID NO：8的氨基酸序列。

    ＨＩＶ의 감염 억제 펩타이드
    15.
    发明公开
    ＨＩＶ의 감염 억제 펩타이드 失效
    抑制艾滋病毒感染的肽

    公开(公告)号：KR1020020035399A

    公开(公告)日：2002-05-11

    申请号：KR1020000065664

    申请日：2000-11-06

    Applicant: 한국과학기술연구원

    Inventor： 유연규 ,  김기선 ,  진봉석

    IPC: A61K38/03

    CPC classification number: A61K38/10

    Abstract: PURPOSE: A peptide for inhibiting HIV infection is provided, which peptide can inhibit the cell fusion of HIV, can be cheaply and easily prepared since it consists of 30 or less of amino acids, and does not need the additional modification for use. CONSTITUTION: The peptide for inhibiting HIV infection comprises an Helix-capping motif peptide capable of binding with 19 amino acids of C-terminal region(amino acids 628 to 646) of gp41 which is a cell membrane fusion protein of HIV, in which the Helix-capping motif peptide has the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 3; the 19 amino acids of C-terminal region of gp41 has the amino acid sequence selected from SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6; and the peptide for inhibiting the infection of HIV has the amino acid sequence selected from SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, and SEQ ID NO: 13.

    Abstract translation: 目的：提供用于抑制HIV感染的肽，该肽可以抑制HIV的细胞融合，因为其由30个或更少的氨基酸组成，因此可以廉价且容易地制备，并且不需要额外的修饰用途。 构成：用于抑制HIV感染的肽包括能够与作为HIV的细胞膜融合蛋白的gp41的C-末端区域（氨基酸628至646）的19个氨基酸结合的螺旋封闭基序肽，其中螺旋 起始基序肽具有SEQ ID NO：2或SEQ ID NO：3的氨基酸序列; gp41的C-末端区域的19个氨基酸具有选自SEQ ID NO：4，SEQ ID NO：5和SEQ ID NO：6的氨基酸序列; 并且用于抑制HIV感染的肽具有选自SEQ ID NO：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10，SEQ ID NO：11，SEQ ID NO： 12和SEQ ID NO：13。

    단백질의 열안정화 방법
    19.
    发明公开
    단백질의 열안정화 방법 失效
    蛋白质热稳定方法

    公开(公告)号：KR1020020032878A

    公开(公告)日：2002-05-04

    申请号：KR1020000063596

    申请日：2000-10-27

    Applicant: 한국과학기술연구원

    Inventor： 김기선 ,  이덕연

    IPC: C12N15/00

    CPC classification number: C12N9/0036 ,  C07K14/195 ,  C07K14/245 ,  C12P21/02

    Abstract: PURPOSE: Provided is a method for thermostabilization of proteins without any change in the structure or function of the proteins. CONSTITUTION: The method for thermostabilization of proteins comprises substituting a hydrophilic residue, aspartic acid, with other hydrophilic residue, glutamic acid on the surface of a water-soluble protein using a site-specific mutagenesis. The site-specific mutagenesis comprises following steps: preparing a protein expression vector, wherein aspartic acids are substituted with glutamic acids using probes of SEQ ID NOs 9 to 14; transforming E. coli with the expression vector; cultivating E. coli transformant in a medium containing Ampicillin at 37 deg.C until the logarithm phase; adding isopropyl beta-D-thiogalactopyranoside into the medium; and sequentially subjecting the cultured medium to nickel nitrilotriacetic acid-agarose affinity column, and DEAE-Sepharose and Superdex G75.

    Abstract translation: 目的：提供蛋白质热稳定的方法，而蛋白质的结构或功能没有任何变化。 构成：蛋白质热稳定的方法包括使用位点特异性诱变将亲水残基，天冬氨酸与其他亲水性残基，水溶性蛋白质表面上的谷氨酸取代。 位点特异性诱变包括以下步骤：使用SEQ ID NO：9至14的探针制备蛋白质表达载体，其中天冬氨酸被谷氨酸取代; 用表达载体转化大肠杆菌; 在37℃下将含有氨苄青霉素的培养基中的大肠杆菌转化体培养至对数期; 向培养基中加入异丙基β-D-硫代吡喃半乳糖苷; 并依次将培养基培养至次氮基三乙酸 - 琼脂糖亲和柱，DEAE-Sepharose和Superdex G75。

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