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公开(公告)号：KR1020090035760A
公开(公告)日：2009-04-13
申请号：KR1020070100702
申请日：2007-10-08
Applicant: 강원대학교산학협력단 , (주)스카이 007
Abstract: A method for preparing oyster peptide is provided to secure effects of whitening and skin regeneration without side effects and prevent corrosion of an apparatus due to no need of thermal treatment using ultrasonic wave. A method for preparing oyster peptide using ultrasonic wave comprises the following steps of: extracting oyster powder with hot water of 40°C, treating the extracts using 90KHZ of an ultrasonic extractor for 10 minutes and pepsin-treating the ultrasonic wave-treated extracts at 37°C for 2 hours to prepare an oyster peptide group; and separating peptide with whitening activity from the oyster peptide group. In the ultrasonic wave step, the extract is repeated by a process of being exposed to ultrasonic wave for 5 seconds and paused for 5 seconds in a double boiler.
Abstract translation: 提供了一种制备牡蛎肽的方法，以确保美白和皮肤再生的效果，而不会有副作用，并且由于不需要使用超声波的热处理，防止设备的腐蚀。 使用超声波制备牡蛎肽的方法包括以下步骤：用40℃的热水提取牡蛎粉，用90KHZ的超声提取器处理提取物10分钟，并在37℃下对超声波处理的提取物进行胃蛋白酶处理 ℃2小时制备牡蛎肽组; 并从牡蛎肽组分离具有增白活性的肽。 在超声波步骤中，通过暴露于超声波5秒钟并在双层锅炉中暂停5秒的处理来重复提取。
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公开(公告)号：KR1020080037805A
公开(公告)日：2008-05-02
申请号：KR1020060104909
申请日：2006-10-27
Applicant: 강원대학교산학협력단
CPC classification number: A23L2/04 , A23L2/38 , A23V2002/00 , A23V2200/324 , A23V2250/21
Abstract: Rubus fruit extract having increased immune activity through ultrasonic extraction at low temperature and a fractionation stage is provided to exhibit higher immune activity and increased cell differentiation degree by an increase of differentiation-inducing material through multi-step differentiation. Rubus fruit is extracted in distilled water at 60deg.C for 24hr, ultrasonically extracted at 40KHz for 30min and fractionated to chloroform, ethyl acetate and butanol in order. For an example, Rubus fruit is washed, dried at 25 to 30deg.C in a shade and ground to 150meshes. The Rubus fruit powder is extracted in water at 100deg.C for 12hr, ultrasonically extracted at 40 KHz for 30min, filtered and concentrated under reduced pressure. The Rubus fruit concentrates are fractionated in steps containing chloroform, ethyl acetate and butanol in order, concentrated under reduced pressure and vacuum-dried.
Abstract translation: 通过在低温和分级分离阶段通过超声提取具有增加的免疫活性的鲁氏果实提取物通过多步分化增加分化诱导材料以显示更高的免疫活性和增加的细胞分化程度。 悬浮果实在60℃的蒸馏水中提取24小时，在40KHz下超声提取30分钟，分级分为氯仿，乙酸乙酯和丁醇。 例如，将胡子水果洗涤，在阴凉处25至30℃干燥并研磨至150目。 将芦荟果粉在100℃水中提取12小时，以40KHz超声提取30分钟，过滤并减压浓缩。 将Rubus果实浓缩物按步骤分批分级，依次加入氯仿，乙酸乙酯和丁醇，减压浓缩并真空干燥。
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公开(公告)号：KR101065443B1
公开(公告)日：2011-09-16
申请号：KR1020090033227
申请日：2009-04-16
Applicant: 강원대학교산학협력단
Abstract: 본 발명은 마황의 피부 노화억제 활성을 위한 식용소재 나노입자화 방법 및 그 생산물에 관한 것으로서, 이는 기본적으로 레시틴으로 마황의 저온열수추출물을 나노입자화하는 것을 특징으로 한다.
본 발명에 따르면 마황의 지용성 레시틴 나노 입자화를 통해, 마황 특유의 냄새에 대한 거부감 없이 저온 추출로 용출된 유용 생리 활성 물질이 효과적인 방법으로 피부에 전달될 수 있어, 미백활성 증진을 위한 향장품으로서의 마황의 가치가 크게 증진된다.
마황, 나노입자, 리포솜, 노화방지-
公开(公告)号：KR1020100024624A
公开(公告)日：2010-03-08
申请号：KR1020080083276
申请日：2008-08-26
Applicant: 주식회사강원심층수 , 강원대학교산학협력단
Abstract: PURPOSE: A manufacturing method of a deep sea water nano particle is provided to improve functionality by promoting organism availability using a nano particle with deep water containing water-soluble minerals. CONSTITUTION: A manufacturing method of a deep sea water nano particle comprises the following steps: melting 0.01~2.00% of a compound selected from a group consisting of edible chitoic acid, edible lecithin, and edible gelatin to dichloromethane or chloroform and evaporating and drying the compound at 30~50 deg C; and mixing and dispersing the dried material obtained from the previous step with a desalted deep sea water. The size of the deep sea water nano particle is 50~300 nano meters. The deep sea water nano particle is in a liposome type capable of collecting water-soluble minerals.
Abstract translation: 目的：提供深海水纳米颗粒的制造方法，以通过使用含有水溶性矿物质的深水的纳米颗粒促进生物体的有效性来改善功能。 构成：深海水纳米颗粒的制造方法包括以下步骤：将0.01〜2.00％的选自可食用氯酸，食用卵磷脂和食用明胶的化合物溶解于二氯甲烷或氯仿中，蒸发干燥 化合物在30〜50℃; 并将由前述步骤得到的干燥物质与脱盐深海水混合分散。 深海水纳米颗粒的尺寸为50〜300纳米。 深海水纳米颗粒是能够收集水溶性矿物质的脂质体。
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公开(公告)号：KR100844386B1
公开(公告)日：2008-07-07
申请号：KR1020070018222
申请日：2007-02-23
Applicant: 강원대학교산학협력단
Abstract: A method for preparing a peptide from cuttlefish collagen is provided to produce the peptide having a low molecular weight by minimizing collagen molecules through cavitation of ultrasonic wave before enzyme treatment, cause no corrosion of a device by chemical materials such as an acid, and increase applicability of the cuttle fish, a conventional waste material, as a material for a cosmetic product because the obtained peptide shows more excellent skin wrinkle inhibitory activity than that of the cuttle fish. A method for preparing a peptide which skin wrinkle inhibitory activity comprises the steps of: (a) treating collagen extracted from cuttlefish at a temperature of 20 deg.C with ultrasonic wave of 40KHz for 30 minutes using an ultrasonic extractor; and (b) adding 0.5 wt.% of collagenase to a product obtained from the step(a) and hydrolyzing it for 1 hour at room temperature.
Abstract translation: 提供从墨鱼胶原蛋白制备肽的方法，通过在酶处理之前通过超声波的空化使胶原分子最小化，使化学物质如酸不会腐蚀装置，从而提高低分子量的肽，并且增加适用性 作为化妆品的材料，作为常规的废弃物的鱼，因为所获得的肽显示比鱼汁更好的皮肤皱纹抑制活性。 一种制备皮肤皱纹抑制活性肽的方法，包括以下步骤：（a）使用超声波提取器，在20℃的超声波40KHz的超声波处理20分钟的墨鱼中提取的胶原蛋白30分钟; 和（b）将0.5重量％的胶原酶加入到从步骤（a）获得的产物中，并在室温下水解1小时。
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Patent Agency Ranking
公开(公告)号：KR100697610B1
公开(公告)日：2007-03-22
申请号：KR1020050115490
申请日：2005-11-30
Applicant: 강원대학교산학협력단
Abstract: A spirulina extract is provided to obtain the cultivated spirulina extract having cancer cell growth inhibition effect and human immuno-cell growth promotion activity by culturing spirulina using highly purified deep water. The spirulina extract is prepared by hot water-extracting a spirulina sample cultivated by the spirulina culturing method by adding solvent of ten times of the amount of the spirulina sample thereto, filtering and concentrating it under reduced pressure and then freeze-drying it. The method for culturing the spirulina comprises the steps of: (a) storing deep water at a temperature of 4 deg.C for 7 days with removing precipitated sediment therefrom; (b) passing the deep water through a 0.2 micrometer membrane filter to obtain highly pure deep water; (c) adding 30-50% of the highly purified deep water into a spirulina culture solution; and (d) culturing the spirulina at a temperature of 30-50 deg.C for 15 days.
Abstract translation: 提供螺旋藻提取物以通过使用高度纯化的深水培养螺旋藻来获得具有癌细胞生长抑制作用和人免疫细胞生长促进活性的培养螺旋藻提取物。 螺旋藻提取物是通过用螺旋藻培养法培养的螺旋藻样品热水提取，加入十倍量的螺旋藻样品的溶剂，过滤并浓缩，然后冷冻干燥。 用于培养螺旋藻的方法包括以下步骤：（a）在4℃的温度下将深水储存7天，从其中除去沉淀的沉淀物; （b）将深水通过0.2微米膜过滤器，以获得高纯度的深水; （c）将30-50％的高纯度深水加入到螺旋藻培养液中; 和（d）在30-50℃的温度下培养螺旋藻15天。
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公开(公告)号：KR101868732B1
公开(公告)日：2018-06-18
申请号：KR1020170010070
申请日：2017-01-20
Applicant: 강원대학교산학협력단
IPC: B01D11/04 , A61K36/185 , A01N65/08
CPC classification number: B01D11/0492 , A01N65/08 , A61K36/185
Abstract: 본발명은폴리페놀추출용조성물및 상기조성물을이용하여폴리페놀함량이증가된케나프추출물을제조하는방법에관한것으로, 상기조성물은계면활성제를첨가한물을추출용매로이용함으로써폴리페놀의추출효율을높일수 있다.
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公开(公告)号：KR101150179B1
公开(公告)日：2012-05-29
申请号：KR1020100049084
申请日：2010-05-26
Applicant: 강원대학교산학협력단
Abstract: PURPOSE: An optimal culture medium for Lactobacillus bulgaricus is provided to enhance the antibacterial activity of Codonopsis lanceolatae is provided to improve the yield of functional ingredients. CONSTITUTION: An optimal culture medium for Lactobacillus bulgaricus to ferment Codonopsis lanceolatae contains 25-35 g/L of fructo-oligosaccharides, 35-45 g/L of maltose, 8-12 g/L of soybean cake, 8-12 g/L of malt extract, 4-6 g/L of yeast extract, 4-6 g/L of sodium acetic acid, 7-9 g/L of disodium phosphate, 1-3 g/L of ammnonium citric acid, 1g/L of tween 80, 0.5 g/L of MgSO_4·7H_2O, 1g/L of K_2HPO_4, and 0.5 g/L of MnSO_4·7H_2O.
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29.发明公开더덕의 항균 활성 증진을 목적으로 하는 유산균 락토바실러스 불가리쿠스의 최적배양배지를 이용한 더덕 발효 방법 有权Lactobacillus BULGARICUS用于发酵CODONOPSIS LANCEOLATA的最佳培养基，使用培养基的发酵方法和产品
公开(公告)号：KR1020110129605A
公开(公告)日：2011-12-02
申请号：KR1020100049084
申请日：2010-05-26
Applicant: 강원대학교산학협력단
Abstract: PURPOSE: An optimal culture medium for Lactobacillus bulgaricus is provided to enhance the antibacterial activity of Codonopsis lanceolatae is provided to improve the yield of functional ingredients. CONSTITUTION: An optimal culture medium for Lactobacillus bulgaricus to ferment Codonopsis lanceolatae contains 25-35 g/L of fructo-oligosaccharides, 35-45 g/L of maltose, 8-12 g/L of soybean cake, 8-12 g/L of malt extract, 4-6 g/L of yeast extract, 4-6 g/L of sodium acetic acid, 7-9 g/L of disodium phosphate, 1-3 g/L of ammnonium citric acid, 1g/L of tween 80, 0.5 g/L of MgSO_4·7H_2O, 1g/L of K_2HPO_4, and 0.5 g/L of MnSO_4·7H_2O.
Abstract translation: 目的：提供保加利亚乳杆菌的最佳培养基，以提高党参的抗菌活性，提高功能成分的产量。 构成：保加利亚乳杆菌发酵党参的最佳培养基含有25-35g / L的低聚果糖，35-45g / L的麦芽糖，8-12g / L的大豆饼，8-12g / L 麦芽提取物，4-6g / L酵母提取物，4-6g / L乙酸钠，7-9g / L磷酸二钠，1-3g / L柠檬酸铵，1g / L 吐温80,0.5g / L MgSO4·7H2O，1g / L K_2HPO_4和0.5g / L MnSO4·7H2O。
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公开(公告)号：KR101056352B1
公开(公告)日：2011-08-12
申请号：KR1020080110930
申请日：2008-11-10
Applicant: 강원대학교산학협력단
IPC: A61K36/232 , A61P37/02
Abstract: 본 발명은 고전압 펄스 전기장 기술을 이용한 당귀의 효율적인 추출방법으로, 기존 일반 추출 방법으로는 유효성분을 쉽게 얻지 못한다는 점과, 고온 추출 시 활성 물질을 파괴할 수 있다는 점을 고려하여 선택한 당귀의 효율적인 추출방법에 관한 것이다.
본 발명의 고전압 펄스 전기장 추출방법은 65~80℃에서 25㎸/㎝의 전기장을 5~20분간 2~3회 정도 주어 천연물인 당귀로부터 유효 성분을 뽑아내는 기술이다. 전기 에너지의 단기간 투여 시 활성 물질의 파괴 없이 세포벽만 선택적인 파괴가 가능해 목적하는 활성 물질의 생산성 증대 및 기타 유용 활성의 증진이 가능하다.
본 발명은 종래 초고압 추출에 비하여 고가의 장비를 사용하지 않아도 된다는 점과, 시간을 절약할 수 있다는 점에서 특징을 가진다. 또한 수율과 생리활성 물질을 많이 내는 것으로 알려진 40~60kHz의 저에너지 초음파 추출과 비교했을 때 역시 비용과 시간 면에서 경제적이다. 본 발명의 고전압 펄스 전기장 추출방법을 통하여 얻어진 당귀 등의 천연물은 종래 통상적인 추출 기술에 비해 천연물의 생리활성 물질에 대한 높은 수율을 가진다.
당귀, 고전압 펄스 전기장 추출, 열수추출, 초음파추출, 초고압추출Abstract translation: 本发明是使用高电压脉冲电场技术提取当归的有效方法，旧的公共提取方法是容易它不能与活性成分而获得，考虑到它们可能破坏点和高温提取活性物质所选择的当归有效 提取方法。
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