公开(公告)号：KR100858169B1

公开(公告)日：2008-09-17

申请号：KR1020060056569

申请日：2006-06-22

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김민철 ,  권용국 ,  조성준 ,  이윤정 ,  조영미 ,  권혁만 ,  권준헌 ,  김재홍

IPC: C12Q1/68

Abstract: 본 발명은 오리 간염바이러스 1형(duck hepatitis virus type 1) 유전자의 감별진단방법(differential diagnostic method)에 관한 것으로, 더욱 상세하게는 2C 유전자, 캡시드(capsid) 유전자 및 5'-UTR(untranslated region) 유전자를 표적(target)하는 특이 프라이머를 사용하여 RT-PCR 방법으로 오리 간염바이러스 1형과 유전자형이 다른 오리 간염바이러스 유전자를 감별 진단하는 방법에 관한 것이다. 또한 본 발명에 따른 감별진단방법은 조류 유래의 다른 병원성 RNA 바이러스들과도 신속하고 정확하게 감별적으로 진단하는데 유용하다.
오리 간염바이러스 1형 및 아형, PCR, 5′-UTR 유전자, 캡시트 유전자, 3D 유전자

公开(公告)号：KR100834014B1

公开(公告)日：2008-06-02

申请号：KR1020060102854

申请日：2006-10-23

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김연희 ,  탁동섭 ,  임성인 ,  현방훈 ,  김인중 ,  송재영 ,  양동군 ,  권창희 ,  권준헌

IPC: C12N7/01 ,  A61K39/12

Abstract: 본 발명은 모기를 매개로 소에서 유사산을 일으키는 소 아까바네병, 츄잔병 및 아이노 바이러스 감염증 예방을 위한 아까바네(K-9), 츄잔(YoungAM) 및 아이노(KSA9910) 바이러스 균주를 이용한 3종 혼합 바이러스 백신 및 그 제조방법에 관한 것으로서, 보다 상세하게는 국내 분리주인 아까바네(K-9), 츄잔(YoungAM) 및 아이노(KSA9910) 바이러스 균주를 불활화시킨 후 이들을 면역증강제와 함께 혼합하여 제조함으로써 3가지 바이러스를 한가지 예방약으로 예방이 가능하도록 제조한 3종 혼합 바이러스 백신 및 그 제조방법에 관한 것이다.
소 아까바네병, 아이노바이러스 감염증, 츄잔병, 3종 혼합불활화 백신

公开(公告)号：KR1020080040942A

公开(公告)日：2008-05-09

申请号：KR1020060108942

申请日：2006-11-06

Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 바이오노트

Inventor： 탁동섭 ,  김성희 ,  권준헌 ,  조수동 ,  양동군 ,  오진식 ,  하건우 ,  조영식

IPC: G01N33/53 ,  G01N33/50 ,  G01N33/48

Abstract: A kit and a method for diagnosing a porcine epidemic diarrhea virus antigen are provided to diagnose the antigen conveniently and rapidly without a special testing equipment in a farm or a laboratory field. A kit for diagnosing a porcine epidemic diarrhea virus antigen is characterized in that a conjugate of a monoclonal antibody 6G15(deposition no. KCTC 10996BP) coupled to nucleocapside 58kDa of an epidemic diarrhea virus and a gold particle is attached to a reaction position of the porcine epidemic diarrhea virus antigen on a strip and a monoclonal antibody 9D11(deposition no. KCTC 10997BP) is attached to a position different from the coupling portion of the porcine epidemic diarrhea virus antigen and the monoclonal antibody 6G15. A method for diagnosing the porcine epidemic diarrhea virus antigen comprises the steps of: (a) loading a sample onto a detection pad on a strip; (b) reacting the conjugate of the monoclonal antibody 6G15 coupled to nucleocapside 58kDa of the epidemic diarrhea virus and the gold particle with the porcine epidemic diarrhea virus antigen in the sample; and (c) reacting the porcine epidemic diarrhea virus antigen, the monoclonal antibody 9D11 and the reaction product obtained from the step(b).

Abstract translation: 提供了一种用于诊断猪流行性腹泻病毒抗原的试剂盒和方法，用于在农场或实验室领域中没有特殊检测设备的情况下方便快速地诊断抗原。 一种用于诊断猪流行性腹泻病毒抗原的试剂盒的特征在于，将与流行性腹泻病毒和金颗粒的核衣壳58kDa偶联的单克隆抗体6G15（沉积编号KCTC 10996BP）的缀合物连接到猪的反应位置 将条带上的流行性腹泻病毒抗原和单克隆抗体9D11（沉积编号KCTC 10997BP）连接到与猪流行性腹泻病毒抗原和单克隆抗体6G15的偶联部分不同的位置。 用于诊断猪流行性腹泻病毒抗原的方法包括以下步骤：（a）将样品加载到条带上的检测垫上; （b）将与流行性腹泻病毒的金卡帕58kDa和金颗粒偶联的单克隆抗体6G15的缀合物与样品中的猪流行性腹泻病毒抗原反应; 和（c）使猪流行性腹泻病毒抗原，单克隆抗体9D11和从步骤（b）获得的反应产物反应。

公开(公告)号：KR1020080036353A

公开(公告)日：2008-04-28

申请号：KR1020060102854

申请日：2006-10-23

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김연희 ,  탁동섭 ,  임성인 ,  현방훈 ,  김인중 ,  송재영 ,  양동군 ,  권창희 ,  권준헌

IPC: C12N7/01 ,  A61K39/12

Abstract: A viral vaccine containing isolated virus strains is provided to improve use safety and immunogenicity by using inactivated viruses, and increase production yield of cows, so that the vaccine is useful for prevention of abortion or stillbirth in cows caused by viruses via mediation of Culicoides spp. A viral vaccine for prevention of abortion or stillbirth in cows contains cultured medium of inactivated virus strains of akabane virus(K-9)(KCTC 18117P), chuzan virus(KSA9910)(KCTC 18118P) and aino virus(YoungAM)(KCTC 18119P), and is produced by: inactivating akabane virus(K-9)(KCTC 18117P), chuzan virus(KSA9910)(KCTC 18118P) and aino virus(YoungAM)(KCTC 18119P) by adding 0.1M BEI(binary ethylene imine) prepared by solubilizing 2-BEA(2-bromoethylamine) in 0.2N NaOH, and treating the solution in a constant temperature water bath of 37 deg. C for 1 hour to them; mixing the 3 kinds of viruses in a weight ratio of 1:1:1; and adding 40-60 wt.% of IMS 1314 as an immunomodulator into the viral mixture.

Abstract translation: 提供含有分离的病毒株的病毒疫苗，以通过使用灭活病毒提高使用安全性和免疫原性，并提高奶牛的产量，使得疫苗可用于通过Culicoides spp的调解来预防由病毒引起的奶牛的流产或死胎。 用于预防奶牛流产或死胎的病毒疫苗含有阿卡巴因病毒（K-9）（KCTC18117P），Chuzan病毒（KSA9910）（KCTC18118P）和Aino病毒（YoungCAM）（KCTC18119P）的灭活病毒株的培养基， 通过添加由0.1M BEI（二元乙烯亚胺）制备的阿卡巴因病毒（K-9）（KCTC 18117P），Chuzan病毒（KSA9910）（KCTC 18118P）和Aino病毒（YoungAM）（KCTC 18119P） 溶解在0.2N NaOH中的2-BEA（2-溴乙胺），并在37℃恒温水浴中处理该溶液。 C给他们1小时; 混合3种病毒，重量比为1：1：1; 并将40-60重量％的IMS 1314作为免疫调节剂加入到病毒混合物中。

公开(公告)号：KR1020080010771A

公开(公告)日：2008-01-31

申请号：KR1020060071258

申请日：2006-07-28

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 권창희 ,  양동군 ,  임성인 ,  탁동섭 ,  권준헌

IPC: C12N15/12

Abstract: Recombinant proteins of enzootic bovine leukemia virus are provided to improve specificity and sensitivity of detection as compared with conventional antigens by expressing the envelope glycoproteins in baculovirus and secreting the envelope glycoproteins to insect cells. A method for producing a recombinant protein of enzootic bovine leukemia virus expressed in an insect cell comprises the steps of: (1) cloning an envelop glycoprotein of enzootic bovine leukemia virus from the blood of enzootic bovine leukemia positive cows to baculovirus and sequencing the cloned protein; (2) transforming an insect cell with a recombinant virus gene of SEQ ID NO:1 to express a recombinant protein of SEQ ID NO:2; and (3) purifying and concentrating the recombinant protein produced from the insect cell, wherein the recombinant virus gene of SEQ ID NO:1 is prepared by recombination of envelop glycoprotein genes including a whole gp51 gene and a part of gp30 gene.

Abstract translation: 通过在杆状病毒中表达包膜糖蛋白并将包膜糖蛋白分泌到昆虫细胞中，提供了特异性和灵敏度的检测与常规抗原的特异性和灵敏度。 用于生产在昆虫细胞中表达的特异性牛白血病病毒重组蛋白质的方法包括以下步骤：（1）将来自地方性牛白血病阳性牛的血液中的特异性牛白血病病毒的包膜糖蛋白克隆至杆状病毒，并测序克隆的蛋白质 ; （2）用SEQ ID NO：1的重组病毒基因转化昆虫细胞以表达SEQ ID NO：2的重组蛋白; （3）从昆虫细胞产生的重组蛋白质的纯化和浓缩，其中通过包括整个gp51基因和一部分gp30基因的包膜糖蛋白基因的重组制备SEQ ID NO：1的重组病毒基因。

公开(公告)号：KR1020070072945A

公开(公告)日：2007-07-10

申请号：KR1020060000325

申请日：2006-01-03

Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 바이오노트

Inventor： 이윤정 ,  최준구 ,  이은경 ,  권용국 ,  권준헌 ,  김재홍 ,  성환우 ,  오진식 ,  조영식 ,  조병기

IPC: G01N33/53

Abstract: A diagnostic strip for a H5 type highly pathogenic avian influenza and general avian influenza is provided to conveniently and rapidly diagnose whether a bird is infected by the H5 type highly pathogenic avian influenza and general avian influenza or not from stool in situ without special testing equipment. The diagnostic strip is characterized in that a monoclonal antibody common to general avian influenza virus in which hemaglutinin antigens are H1 to H15 and a monoclonal antibody specific to a highly pathogenic avian influenza in which a hemaglutinin antigen is H5 are used for diagnosing the highly pathogenic and the general avian influenza in which the hemaglutinin antigen is H5 from stool or tissue of chicken. The method for preparing the diagnostic strip comprises the steps of: (a) respectively absorbing the monoclonal antibody specific to the highly pathogenic avian influenza virus in which the hemaglutinin antigen is H5 and the monoclonal antibody common to general avian influenza virus into a membrane; (b) respectively attaching the antibody absorbed membrane obtained from the step(a) to gold particles so as to prepare an antibody-gold conjugate and then mixing it; and (c) precipitating the conjugate obtained from the step(b) in a basic material and then drying it.

Abstract translation: 提供一种H5型高致病性禽流感和一般禽流感的诊断条，以方便快捷地诊断鸟类是否由H5型高致病性禽流感和一般禽流感感染，或者不是在没有特殊检测设备的情况下从粪便中感染。 诊断条的特征在于，其中血凝素抗原为H1至H15的一般性禽流感病毒共有的单克隆抗体和其中血凝素抗原为H5的高致病性禽流感特异性的单克隆抗体用于诊断高致病性和 其中血凝素抗原为粪便或鸡组织中H5的一般性禽流感。 制备诊断条的方法包括以下步骤：（a）分别将其中血凝素抗原为H5的高致病性禽流感病毒和一般禽流感病毒共有的单克隆抗体特异性的单克隆抗体吸收到膜中; （b）将由步骤（a）获得的抗体吸收膜分别附着于金颗粒上，制备抗体 - 金缀合物，然后混合; 和（c）将由步骤（b）获得的缀合物沉淀在碱性材料中，然后干燥。

公开(公告)号：KR1020070005867A

公开(公告)日：2007-01-10

申请号：KR1020050060920

申请日：2005-07-06

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 성환우 ,  이윤정 ,  최준구 ,  이은경 ,  권준헌 ,  김재홍

IPC: C12N7/01 ,  C12N7/04

Abstract: A virus strain for preparing a vaccine is provided to have excellent defending effect on low pathogenic avian influenza H9N2 virus strain. And a vaccine is provided to be able to effectively prevent diseases caused by the low pathogenic avian influenza H9N2 recording the high mortality of bird. The low pathogenic avian influenza H9N2 virus strain for preparing vaccine is characterized in that a motif of hemangioblast protein cleavage site amino acid sequence has TSGR and it shows the defending capacity against domestic low pathogenic avian influenza virus. The method for preparing a vaccine for the low pathogenic avian influenza H9N2 virus strain comprises the steps of: (a) inactivating the low pathogenic avian influenza H9N2 virus strain; and (b) mixing the inactivated virus with an adequate excipient.

Abstract translation: 提供用于制备疫苗的病毒株，以对低致病性禽流感H9N2病毒株具有优异的防御作用。 并提供疫苗能够有效预防由低致病性禽流感H9N2引起的疾病，记录鸟类的高死亡率。 用于制备疫苗的低致病性禽流感H9N2病毒株的特征在于成血管细胞蛋白切割位点氨基酸序列的基序具有TSGR，并且显示出针对国内低致病性禽流感病毒的防御能力。 用于制备低致病性禽流感H9N2病毒株的疫苗的方法包括以下步骤：（a）灭活低致病性禽流感H9N2病毒株; 和（b）将灭活的病毒与足够的赋形剂混合。

公开(公告)号：KR100593255B1

公开(公告)日：2006-06-28

申请号：KR1020040033171

申请日：2004-05-11

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 권창희 ,  강현미 ,  장금찬 ,  문진산 ,  강승원 ,  권준헌 ,  김종만

IPC: C12N1/20

Abstract: 본 발명은 바실러스 에스피 87 (KACC 91098) 및 이의 용도에 관한 것으로서, 상기 균주는 내산성, 내담즙성 및 생체 안전성이 인정되고, 병원성 세균 억제활성을 가지며, 장내에서 독립된 균총(Niche)으로서 미생물의 길항 작용을 통한 병원성 균총의 증식을 감소시켜 질병을 예방하는 효과가 있다.

公开(公告)号：KR100543691B1

公开(公告)日：2006-01-20

申请号：KR1020020079085

申请日：2002-12-12

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 송재영 ,  최은진 ,  박종현 ,  안동준 ,  차상호 ,  권준헌

IPC: G01N33/543

Abstract: 본 발명은 항원-항체 반응을 이용하여 신속하게 돼지콜레라바이러스에 대한 항체의 존재유무를 검출할 수 있도록 고안된 검출 키트 및 이를 이용한 돼지콜레라바이러스 항체의 검출 방법에 관한 것이다.
돼지콜레라, 면역크로마토그라피, 골드 콘쥬게이트, 유전자 재조합

公开(公告)号：KR100267745B1

公开(公告)日：2000-11-01

申请号：KR1019980000447

申请日：1998-01-10

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 박종현 ,  송재영 ,  안동준 ,  현방훈 ,  차상호 ,  안수환 ,  권준헌

IPC: C12N15/33

Abstract: PURPOSE: Provided is a spike gene and a method for detecting neutralizing antibodies of porcine epidemic diarrhea virus to prevent porcine epidemic diarrhea by using spike proteins which are mass-produced through genetic engineering. CONSTITUTION: The spike gene obtained from porcine epidemic diarrhea virus is located in the gene of recombinant baculovirus (KFCC 11014). And a method for detecting neutralizing antibodies of porcine epidemic diarrhea virus (PEDV) is characterized by the next step of: using a spike protein expressed in recombinant baculovirus (KFCC 11014) as an antigen in implementing indirect-ELISA. A method for manufacturing the recombinant baculovirus (KFCC 11014) is comprised of the following steps of: i) making expression vector pVL 1393 having a spike gene of PEDV and obtaining a medium lacking serum but including linear baculovirus, and mixture containing lipopectin; and ii) cotransfecting host cell Sf9 using the mixture.

Abstract translation: 目的：提供一种尖峰基因和一种检测猪流行性腹泻病毒中和抗体的方法，通过使用通过基因工程大规模生产的穗蛋白来预防猪流行性腹泻。 构成：从猪流行性腹泻病毒获得的穗基因位于重组杆状病毒（KFCC 11014）的基因中。 检测猪流行性腹泻病毒（PEDV）中和抗体的方法的特征在于：使用重组杆状病毒（KFCC 11014）中表达的尖峰蛋白作为抗原进行间接ELISA。 制备重组杆状病毒（KFCC 11014）的方法包括以下步骤：i）制备具有PEDV刺突基因的表达载体pVL1393，获得缺乏血清但包括线性杆状病毒的培养基，以及含有脂蛋白的混合物; 和ii）使用该混合物共转染宿主细胞Sf9。