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    MODIFIED NUCLEOTIDES AND USES THEREOF
    2.
    发明申请
    MODIFIED NUCLEOTIDES AND USES THEREOF 审中-公开
    改良的核苷酸及其用途

    公开(公告)号：WO2016153999A1

    公开(公告)日：2016-09-29

    申请号：PCT/US2016/023139

    申请日：2016-03-18

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES GMBH

    Inventor： HINZ, Wolfgang ,  MENCHEN, Steven ,  GRAHAM, Ronald ,  VANDER HORN, Peter ,  HUBBELL, Earl ,  WOEHLER, Christian

    IPC: C12Q1/68 ,  G01N27/414

    CPC classification number: C12Q1/6869 ,  G01N27/4145 ,  C12Q2525/117 ,  C12Q2565/30

    Abstract: In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. Optionally, the array contains a plurality of reaction sites having about 1-100 million, or about 100-250 million, or about 200-500 million, or about 500-900 million, or more reaction sites. Optionally, each reaction site is in contact with, operatively coupled, or capacitively coupled to one or more sensors that are ion-sensitive FETs (isFETs) or chemically-sensitive FETs (chemFETs) sensors. Optionally, the reaction sites are in fluid communication with each other.

    Abstract translation: 在一些实施方案中，本公开一般涉及涉及在模板依赖性中使用经修饰的核苷酸（包括终止子核苷酸和/或标记的核苷酸）的核酸分析的方法以及相关的系统，组合物，试剂盒和装置 核苷酸掺入反应。 在一些实施方案中，核酸分析可以在单个反应位点或反应位点阵列中的多个反应位点进行。 任选地，阵列包含多个具有约1-100百万，约100-250百万，或约200-500百万，或约500-900万个或更多个反应位点的反应位点。 任选地，每个反应位点与作为离子敏感FET（isFET）或化学敏感FET（chemFET）传感器的一个或多个传感器接触，可操作地耦合或电容耦合。 任选地，反应位点彼此流体连通。

    CONJUGATED POLYMERIC PARTICLE AND METHOD OF MAKING SAME
    5.
    发明申请
    CONJUGATED POLYMERIC PARTICLE AND METHOD OF MAKING SAME 审中-公开
    聚合聚合物颗粒及其制备方法

    公开(公告)号：WO2013119956A1

    公开(公告)日：2013-08-15

    申请号：PCT/US2013/025352

    申请日：2013-02-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

    IPC: C12Q1/68

    CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

    Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a non¬ aqueous solvent, and coupling the biomolecule complex to a sub¬ strate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immo¬ bilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

    Abstract translation: 缀合底物的方法包括将与生物分子相关的抗衡离子与亲脂性反离子交换以形成生物分子复合物，将生物分子复合物分散在非水溶剂中，并在存在下将生物分子复合物偶联到亚基 的非水溶剂。 底物或固体支持物是聚合物颗粒，待沉淀在颗粒上的生物分子是寡核苷酸，亲脂性抗衡离子是四丁基铵离子，非水溶剂是N-甲基吡咯烷酮（NMP）。 寡核苷酸包被的颗粒用于核酸分离或核酸测序反应。

    MESYLATE BASED MASTER MIX
    7.
    发明申请
    MESYLATE BASED MASTER MIX 审中-公开

    公开(公告)号：WO2018237024A1

    公开(公告)日：2018-12-27

    申请号：PCT/US2018/038531

    申请日：2018-06-20

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： ZHONG, Chang ,  MULERO, Julio ,  MENCHEN, Steven

    IPC: C12Q1/686 ,  C12Q1/6848

    Abstract: Disclosed herein are mesylate containing compositions, methods of use of such compositions and kits of components encompassing such compositions for improving the amplification of nucleic acids, especially in the presence of enzymatic inhibitors. These find particular use in forensic and environmental analyses. The instant disclosure provides new compositions, methods and kits of components for overcoming PCR inhibition. In some embodiments disclosed herein is a PCR master mix, the PCR master mix encompassing a mesylate.

    THIOLATED NUCLEOTIDE ANALOGUES FOR NUCLEIC ACID SYNTHESIS
    8.
    发明申请
    THIOLATED NUCLEOTIDE ANALOGUES FOR NUCLEIC ACID SYNTHESIS 审中-公开
    用于核酸合成的硫酸核苷酸类似物

    公开(公告)号：WO2016077324A1

    公开(公告)日：2016-05-19

    申请号：PCT/US2015/059921

    申请日：2015-11-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MENCHEN, Steven ,  NIKIFOROV, Theo

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  G01N27/4145 ,  C12Q2525/125 ,  C12Q2535/113 ,  C12Q2565/301 ,  C12Q2565/607

    Abstract: The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety.

    Abstract translation: 本公开提供用于扩展核酸的系统，组合物，方法，试剂，试剂盒和产品，其包括使用聚合酶和至少一个核苷酸在核酸的末端掺入核苷酸残基，其中所述至少一个核苷酸包括 硫代磷酸酯部分，并且其中所述至少一个核苷酸耐磷酸酶水解。 在一些实施方案中，核苷酸掺入可以在磷酸酶的存在下进行。 在一些实施方案中，核苷酸掺入可以在配置成结合正磷酸盐部分的至少一个螯合部分的存在下进行。

    POLYMER PARTICLES AND METHODS OF MAKING AND USING SAME
    10.
    发明申请
    POLYMER PARTICLES AND METHODS OF MAKING AND USING SAME 审中-公开
    聚合物颗粒及其制备方法和使用方法

    公开(公告)号：WO2012142132A1

    公开(公告)日：2012-10-18

    申请号：PCT/US2012/033084

    申请日：2012-04-11

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  MENCHEN, Steven ,  MASTROIANNI, Alexander

    Inventor： MENCHEN, Steven ,  MASTROIANNI, Alexander

    IPC: B01J13/00 ,  C08J3/00

    CPC classification number: C12N15/1093 ,  C08F2/22 ,  C08F220/56 ,  C08L33/26 ,  Y10T428/2982

    Abstract: A method of making polymer particles includes making an aqueous gel reaction mixture; forming an emulsion comprising dispersed aqueous phase micelles of gel reaction mixture in a continuous phase at a temperature less than about 10°C; and performing a polymerization reaction in the micelles. Further, the emulsion comprises at least one polymerization initiator in the micelles of gel reaction mixture. The gel reaction mixture can be maintained at a temperature less than about 10°C when it comprises the polymerization initiator.

    Abstract translation: 制备聚合物颗粒的方法包括制备水凝胶反应混合物; 在小于约10℃的温度下，在连续相中形成包含分散的凝胶反应混合物的水相胶束的乳液; 并在胶束中进行聚合反应。 此外，乳液在凝胶反应混合物的胶束中包含至少一种聚合引发剂。 当凝胶反应混合物包含聚合引发剂时，可将其保持在低于约10℃的温度。

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