公开(公告)号：AU2011326026A1

公开(公告)日：2013-05-02

申请号：AU2011326026

申请日：2011-11-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  KORLACH JONAS ,  WEGENER JEFFREY ,  CLARK TYSON A ,  VILFAN IGOR ,  KISLYUK ANDREY ,  TURNER STEPHEN ,  SORENSON JON ,  TRAVERS KEVIN ,  HEINER CHERYL ,  TOMANEY AUSTIN B ,  MARKS PATRICK ,  LEE JESSICA ,  JIA LEI ,  WEBSTER DALE ,  LYLE JOHN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/48

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：AU2006320414B2

公开(公告)日：2012-10-04

申请号：AU2006320414

申请日：2006-12-01

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： OTTO GEOFFREY ,  EID JOHN ,  TURNER STEPHEN ,  MURPHY DEVON

IPC: G01N33/53 ,  C12N11/02

Abstract: Compositions, devices, systems and methods for reducing and/or preventing photodamage of one or more reactants in illuminated analytical reactions by one or more of incorporating photodamage mitigating agents within the reaction mixture and/or interrogating different observation regions of the reaction mixture for a period that is less than a photodamage threshold period.

公开(公告)号：AU2011217862A1

公开(公告)日：2012-09-27

申请号：AU2011217862

申请日：2011-02-18

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： MCCAFFREY NATHANIEL JOSEPH ,  TURNER STEPHEN ,  SAXENA RAVI ,  HELGESEN SCOTT EDWARD

IPC: G01N21/00

Abstract: An analytical assembly within a unified device structure for integration into an analytical system. The analytical assembly is scalable and includes a plurality of analytical devices, each of which includes a reaction cell, an optical sensor, and at least one optical element positioned in optical communication with both the reaction cell and the sensor and which delivers optical signals from the cell to the sensor. Additional elements are optionally integrated into the analytical assembly. Methods for forming and operating the analytical system are also disclosed.

公开(公告)号：AU2007241049B2

公开(公告)日：2012-07-05

申请号：AU2007241049

申请日：2007-03-29

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TURNER STEPHEN ,  OTTO GEOFF ,  XU YUE ,  KORLACH JONAS ,  RANK DAVID R ,  ROITMAN DANIEL ,  LYLE JOHN ,  PELUSO PAUL ,  WEGENER JEFFERY ,  CICERO RONALD L

IPC: C12N11/00 ,  C40B40/04

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

公开(公告)号：AT548643T

公开(公告)日：2012-03-15

申请号：AT08742395

申请日：2008-03-27

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： KORLACH JONAS ,  SAMIEE KEVAN ,  TURNER STEPHEN ,  FREUDENTHAL JAKE

IPC: G01N21/64

Abstract: Composition, systems, apparatus and methods of enhancing fluorescent signals in biochemical are described. Metal particle proximity to enzymes that produce fluorescent products provide enhanced fluorescence of the product and plasmon resonance of the metal particle. Multi-labeled nucleotides enhance signal production. Reflectance of illumination light and emitted fluorescence increase signal strength for a given illumination light.

公开(公告)号：AT546522T

公开(公告)日：2012-03-15

申请号：AT07754529

申请日：2007-03-29

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： RANK DAVID ,  WEGENER JEFFERY ,  KORLACH JONAS ,  ROITMAN DANIEL ,  XU YUE ,  LYLE JOHN ,  TURNER STEPHEN ,  PELUSO PAUL ,  OTTO GEOFF ,  CICERO RONALD

IPC: C12N11/00 ,  C40B40/04

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

公开(公告)号：AU2006331512B2

公开(公告)日：2012-02-23

申请号：AU2006331512

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： KORLACH JONAS ,  OTTO GEOFFREY ,  HANZEL DAVID ,  PELUSO PAUL ,  TURNER STEPHEN ,  RANK DAVID ,  PHAM THANG

IPC: C12Q1/68 ,  C12N11/14

Abstract: Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.

公开(公告)号：AU2009300369A1

公开(公告)日：2010-04-08

申请号：AU2009300369

申请日：2009-09-25

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： ZACCARIN DENIS ,  LUNDQUIST PAUL ,  ZHAO PEIQIAN ,  ZHONG FRANK ,  TURNER STEPHEN ,  HUANG YANQIAO ,  MONADGEMI PEZHMAN ,  SAXENA RAVI ,  GROT ANNETTE ,  RULISON AARON

IPC: G01N21/00 ,  G02B6/12

公开(公告)号：CA2700838A1

公开(公告)日：2009-04-09

申请号：CA2700838

申请日：2008-09-26

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： HEINER CHERYL ,  TURNER STEPHEN ,  TRAVERS KEVIN

IPC: C12Q1/68 ,  C07H21/04

Abstract: Methods of preparing nucleic acid templates and providing the nucleic acid templates to low copy number reaction volumes are provided. Related compositions of nucleic acid templates are also provided.

公开(公告)号：CA2662521A1

公开(公告)日：2008-03-06

申请号：CA2662521

申请日：2007-08-31

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： LUNDQUIST PAUL ,  TURNER STEPHEN

IPC: F21V9/16

Abstract: Substrates, systems and methods for analyzing materials that include wave guide arrays disposed upon or within the substrate such that evanescent fiel ds emanating from the waveguides illuminate materials disposed upon or proxi mal to the surface of the substrate, permitting analysis of such materials. The substrates, systems and methods are used in a variety of analytical oper ations, including, inter alia, nucleic acid analysis, including hybridizatio n and sequencing analyses, cellular analyses and other molecular analyses.