公开(公告)号：AU2009292629B2

公开(公告)日：2014-03-20

申请号：AU2009292629

申请日：2009-09-15

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZHONG CHENG FRANK ,  LUNDQUIST PAUL ,  FOQUET MATHIEU ,  KORLACH JONAS ,  BAYANDORIAN HOVIG

IPC: G01N21/00 ,  G01B9/02 ,  G01N33/483

Abstract: This invention provides substrates for use in various applications, including single-molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided.

公开(公告)号：AU2009223702B2

公开(公告)日：2013-08-22

申请号：AU2009223702

申请日：2009-03-12

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： WEGENER JEFFREY ,  KORLACH JONAS

IPC: G01N33/532 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/533 ,  G01N33/535

Abstract: Labeled reactant compositions, and particularly labeled nucleic acid reaction compositions, that include structural components that maintain potentially damaging labeling components sufficiently distal from the reactant portion of the molecule such that damaging effects of the label group on other reaction components, such as enzymes, are reduced, minimized and/or eliminated.

公开(公告)号：AU2011326026A1

公开(公告)日：2013-05-02

申请号：AU2011326026

申请日：2011-11-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  KORLACH JONAS ,  WEGENER JEFFREY ,  CLARK TYSON A ,  VILFAN IGOR ,  KISLYUK ANDREY ,  TURNER STEPHEN ,  SORENSON JON ,  TRAVERS KEVIN ,  HEINER CHERYL ,  TOMANEY AUSTIN B ,  MARKS PATRICK ,  LEE JESSICA ,  JIA LEI ,  WEBSTER DALE ,  LYLE JOHN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/48

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：AU2007241049B2

公开(公告)日：2012-07-05

申请号：AU2007241049

申请日：2007-03-29

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TURNER STEPHEN ,  OTTO GEOFF ,  XU YUE ,  KORLACH JONAS ,  RANK DAVID R ,  ROITMAN DANIEL ,  LYLE JOHN ,  PELUSO PAUL ,  WEGENER JEFFERY ,  CICERO RONALD L

IPC: C12N11/00 ,  C40B40/04

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

公开(公告)号：AT548643T

公开(公告)日：2012-03-15

申请号：AT08742395

申请日：2008-03-27

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： KORLACH JONAS ,  SAMIEE KEVAN ,  TURNER STEPHEN ,  FREUDENTHAL JAKE

IPC: G01N21/64

Abstract: Composition, systems, apparatus and methods of enhancing fluorescent signals in biochemical are described. Metal particle proximity to enzymes that produce fluorescent products provide enhanced fluorescence of the product and plasmon resonance of the metal particle. Multi-labeled nucleotides enhance signal production. Reflectance of illumination light and emitted fluorescence increase signal strength for a given illumination light.

公开(公告)号：AT546522T

公开(公告)日：2012-03-15

申请号：AT07754529

申请日：2007-03-29

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： RANK DAVID ,  WEGENER JEFFERY ,  KORLACH JONAS ,  ROITMAN DANIEL ,  XU YUE ,  LYLE JOHN ,  TURNER STEPHEN ,  PELUSO PAUL ,  OTTO GEOFF ,  CICERO RONALD

IPC: C12N11/00 ,  C40B40/04

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

公开(公告)号：AU2006331512B2

公开(公告)日：2012-02-23

申请号：AU2006331512

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： KORLACH JONAS ,  OTTO GEOFFREY ,  HANZEL DAVID ,  PELUSO PAUL ,  TURNER STEPHEN ,  RANK DAVID ,  PHAM THANG

IPC: C12Q1/68 ,  C12N11/14

Abstract: Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.

公开(公告)号：EP2370598A4

公开(公告)日：2012-06-13

申请号：EP09832251

申请日：2009-12-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： FLUSBERG BENJAMIN ,  TURNER STEPHEN ,  LEE JESSICA ,  JIA LEI ,  KORLACH JONAS ,  SORENSON JON ,  WEBSTER DALE ,  LYLE JOHN ,  TRAVERS KEVIN ,  HANES JEREMIAH

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6869 ,  C12Q1/6837 ,  C12Q1/6858 ,  C12Q2561/113 ,  C12Q2537/164 ,  C12Q2527/113 ,  C12Q2521/101 ,  C12Q2525/307 ,  C12Q2525/117

公开(公告)号：EP2217928A4

公开(公告)日：2011-02-09

申请号：EP08856043

申请日：2008-12-03

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： KORLACH JONAS ,  ROITMAN DANIEL ,  EID JOHN ,  OTTO GEOFF ,  HARDENBOL PAUL ,  FLUSBERG BENJAMIN

IPC: G01N33/573 ,  B01L3/00 ,  C08G83/00 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/535 ,  G01N33/542 ,  G01N33/543 ,  G01N33/58

CPC classification number: G01N33/54373 ,  C12Q1/6874 ,  G01N33/542 ,  G01N33/54313 ,  G01N33/573 ,  G01N33/58 ,  G01N33/585 ,  C12Q2565/607 ,  C12Q2565/531 ,  C12Q2563/143 ,  C12Q2563/137

公开(公告)号：CA2974241C

公开(公告)日：2020-01-07

申请号：CA2974241

申请日：2009-09-15

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZHONG CHENG FRANK ,  LUNDQUIST PAUL ,  FOQUET MATHIEU ,  KORLACH JONAS ,  BAYANDORIAN HOVIG

IPC: G01N21/17 ,  B82Y20/00 ,  C12M1/34 ,  C12Q1/00 ,  G01N21/00 ,  G01N21/63 ,  G01N21/77 ,  G02B6/12

Abstract: This specification provides substrates for use in various applications, including single-molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided.