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公开(公告)号：KR1020110037772A
公开(公告)日：2011-04-13
申请号：KR1020090095334
申请日：2009-10-07
Applicant: 한국생명공학연구원 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A method for identifying Mealybug in a Korean ship is provided to accurately and quickly identify Mealybug by PCR. CONSTITUTION: A primer set for PCR to identify Mealybug contains: a primer set comprising a forward primer of sequence number 1 and a reverse primer of sequence number 2; a primer set comprising a forward primer of sequence number 3 and a reverse primer of sequence number 4; a primer set comprising a forward primer of sequence number 5 and a reverse primer of sequence number 6; a primer set comprising a forward primer of sequence number 7 and a reverse primer of sequence number 8; a primer set comprising a forward primer of sequence number 9 and a reverse primer of sequence number 10; and a primer set comprising a forward primer of sequence number 11 and a reverse primer of sequence number 12. A method for identifying Mealybug comprises: a step of isolating genomic DNA from Mealybug or larva thereof; a step of performing PCR using the primer; and a step of confirming PCR products.
Abstract translation: 目的：提供一种用于识别韩国船只中的Mealybug的方法，以通过PCR准确快速地识别Mealybug。 构成：用于鉴定Mealybug的PCR引物组包含：引物组，其包含序列号1的正向引物和序列号2的反向引物; 包含序列号3的正向引物和序列号4的反向引物的引物组; 包含序列号5的正向引物和序列号6的反向引物的引物组; 包含序列号7的正向引物和序列号8的反向引物的引物组; 包含序列号9的正向引物和序列号10的反向引物的引物组; 以及引物组，其包含序列号11的正向引物和序列号12的反向引物。鉴定Mealybug的方法包括：从Mealybug或其幼虫中分离基因组DNA的步骤; 使用引物进行PCR的步骤; 以及确认PCR产物的步骤。
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公开(公告)号：KR1020100136191A
公开(公告)日：2010-12-28
申请号：KR1020090054422
申请日：2009-06-18
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: An antigen for hemoglutination for swine Japanese encephalitis virus is provided to reduce pollution caused by acetone extraction. CONSTITUTION: A method for preparing antigen for hemoglutination of high concentration for swine Japanese encephalitis virus comprises: a step of inoculating swine Japanese encephalitis virus to a tissue culture cells and planting; a step of freezing culture liquid; a step of thawing the culture liquid and repeating freezing and thawing once; a step of collecting cultured swine Japanese encephalitis virus; a step of inactivating swine Japanese encephalitis virus; a step of concentrating swine Japanese encephalitis virus; and a step of precipitating swine Japanese encephalitis virus by centrifugation.
Abstract translation: 目的：提供用于猪日本脑炎病毒血凝反应的抗原，以减少丙酮提取引起的污染。 构成：一种制备猪日本脑炎病毒高浓度血凝反应抗原的方法，包括：将日本脑炎病毒接种在组织培养细胞中并种植; 冷冻培养液的一个步骤; 解冻培养液并重复冻融一次; 收集日本猪日本脑炎病毒的一步; 灭活日本脑炎病毒的一步; 日本脑炎病毒浓缩一步; 并通过离心沉淀猪日本脑炎病毒的一个步骤。
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公开(公告)号：KR1020100121289A
公开(公告)日：2010-11-17
申请号：KR1020090040369
申请日：2009-05-08
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A recombinant Flc-LOM swine fever vaccine virus is provided to easily detect other vaccine virus and outdoor virus and to early detect infected pig. CONSTITUTION: A vaccine virus for swine fever, Flc-LOM virus(KFCC11441P) has a nucleotide sequence of sequence number 1. In the vaccine virus, 52, 96, 220, 405th bases are changed from C, A, G, and G to T, G, T, and A. The vaccine virus Flc-LOM genetic marker or END(Exaltaton of Newcastle Disease virus). A live vaccine for swine fever contains the vaccine virus Flc-LOM virus(KFCC 11441P).
Abstract translation: 目的：提供重组Flc-LOM猪瘟疫苗病毒，轻松检测其他疫苗病毒和户外病毒，及早发现感染猪。 构成：用于猪瘟的疫苗病毒Flc-LOM病毒（KFCC11441P）具有序列号1的核苷酸序列。在疫苗病毒中，52,96,220,405碱基从C，A，G和G改变为 T，G，T和A.疫苗病毒Flc-LOM遗传标记或END（新城疫病毒）。 用于猪瘟的活疫苗包含疫苗病毒Flc-LOM病毒（KFCC 11441P）。
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公开(公告)号：KR100983873B1
公开(公告)日：2010-10-01
申请号：KR1020080064772
申请日：2008-07-04
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12Q1/68
Abstract: 본 발명은 돼지이유후전신성소모성증후군 또는 돼지피부염신부전증후군으로부터 돼지 써코바이러스 1 및 2를 감별진단하는 방법에 관한 것으로서, 보다 상세하게는 돼지 써코바이러스 타입 1과 2의 DNA 및 공통 프로브로 구성되는 나일론막을 이용하는 미니어레이법을 도입하여 돼지이유후전신성소모성증후군 또는 돼지피부염신부전증후군으로부터 돼지 써코바이러스 1 및 2를 정확하게 감별진단하는 방법에 관한 것이다.
미니어레이법, 돼지 써코바이러스 감별진단법-
公开(公告)号：KR1020100102764A
公开(公告)日：2010-09-27
申请号：KR1020090020979
申请日：2009-03-12
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A monoclonal antibody which binds to glycoprotein of vesicular stomatitis virus serotype New Jersey is provided to accurately and quickly diagnose neutralizing antibody of vesicular stomatitis virus serotype New Jersey. CONSTITUTION: A monoclonal antibody VSVNJ 1G11 neutralizes vesicular stomatitis virus serotype New Jersey by binding to glycoprotein comprising outer surface of vesicular stomatitis virus serotype New Jersey. The monoclonal antibody VSVNJ 1G11 is prepared by fusion cell line KCLRF-BP-00199. The fusion cell line(deposit number: KCLRF-BP-00199) is prepared by cell fusion of B cells and mouse myeloma-derived cells. A composition for treating vesicular stomatitis contains the monoclonal antibody VSVNJ 1G11.
Abstract translation: 目的：提供结合水泡性口炎病毒血清型新泽西州糖蛋白的单克隆抗体，准确快速诊断水泡性口炎病毒血清型新泽西中和抗体。 构成：单克隆抗体VSVNJ 1G11通过结合包含水泡性口炎病毒血清型新泽西的外表面的糖蛋白中和水泡性口炎病毒血清型新泽西。 单克隆抗体VSVNJ 1G11通过融合细胞系KCLRF-BP-00199制备。 通过B细胞和小鼠骨髓源性细胞的细胞融合制备融合细胞系（保藏号：KCLRF-BP-00199）。 用于治疗囊泡性口炎的组合物含有单克隆抗体VSVNJ 1G11。
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Patent Agency Ranking
公开(公告)号：KR1020100082975A
公开(公告)日：2010-07-21
申请号：KR1020090002301
申请日：2009-01-12
Applicant: 대한민국(농촌진흥청장) , 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: C12N15/10 , C12N15/11 , C12Q1/6851 , C12Q1/6853 , C12Q1/701
Abstract: PURPOSE: A primer set for diagnosing tobacco rattle virus(TRV) and a method for diagnosing TRV using the same are provided to accurately diagnose TRV infection from Solanaceae seed or plant. CONSTITUTION: A primer set for diagnosing TRV comprises a forward primer selected from sequence numbers 1, 2, 3, 4, 5 and 6, and reverse primer selected from sequence numbers 9, 10, 11, 12, 13, 14 and 15. The primer set for diagnosing TRV comprises a primer set of sequence number 3 and 1 and primer set of sequence numbers 1 and 14. A method for diagnosing TRV comprises: a step of isolating total RNA from a plant sample; a step of amplifying a target sequence by RT-PCR; and a step of detecting PCR product.
Abstract translation: 目的：提供用于诊断烟草拨鼠病毒（TRV）的引物组和使用其的诊断TRV的方法，以准确诊断茄科种子或植物的TRV感染。 构成：用于诊断TRV的引物组包含选自序列号1,2,3,4,5和6的正向引物，以及选自序列号9,10,11,12,13,14和15的反向引物。 用于诊断TRV的引物组包含序列号3和1的引物组和序列号1和14的引物组。诊断TRV的方法包括：从植物样品中分离总RNA的步骤; 通过RT-PCR扩增靶序列的步骤; 以及检测PCR产物的步骤。
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公开(公告)号：KR1020100043714A
公开(公告)日：2010-04-29
申请号：KR1020080102883
申请日：2008-10-21
Applicant: 건국대학교 산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C07K14/08 , C07K14/005 , C12N15/12 , C12N15/09
Abstract: PURPOSE: A capsid protein of swine hepatitis E virus(HEV) and a gene encoding the same are provided to develop diagnosis and vaccine for HEV. CONSTITUTION: A swine hepatitis E virus(HEV) ORF2 capsid protein contains an amino acid of sequence number 7. A gene encoding the swine HEV ORF2 capsid protein ahs a sequence of sequence number 6. A vector pswHEV-capsid contains a gene encoding the protein. A transformed microorganism is obtained by introducing the vector pswHEV-capsid to host cell of bacteria, fungi, or yeast. The host cell is E.coli. The transformed microorganism is deposit number KCTC18139P.
Abstract translation: 目的：提供猪戊型肝炎病毒（HEV）的衣壳蛋白和编码其的基因，以开发HEV的诊断和疫苗。 构成：猪戊型肝炎病毒（HEV）ORF2衣壳蛋白含有序列号7的氨基酸。编码猪HEV ORF2衣壳蛋白的基因是序列号6的序列。载体pswHEV-衣壳含有编码蛋白质的基因 。 通过将载体pswHEV-衣壳引入细菌，真菌或酵母的宿主细胞获得转化的微生物。 宿主细胞是大肠杆菌。 转化的微生物为保藏号KCTC18139P。
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公开(公告)号：KR100951016B1
公开(公告)日：2010-04-06
申请号：KR1020070108498
申请日：2007-10-26
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 질병 검사에 사용되는 한천겔 플레이트의 제작 장치 및 이를 이용한 한천겔 플레이트의 제작 방법에 관한 것으로, 구체적으로는 진공펌프 등을 이용하여 규격화된 흡입관내로 고형 한천겔을 흡입하게 함으로서 질병검사용 한천겔 플레이트를 대량으로 용이하게 제작하기 위하여 개발된 장치 및 방법에 관한 것이다.
본 발명에 따르면, 한천겔 플레이트를 대량으로 용이하게 제작함으로서 종래의 수작업으로 진행되었던 한천겔 침강반응 플레이트제작이 자동화됨으로서 플레이트 제작이 간편하고 대량으로 손쉽게 제작할 수 있으며, 검사웰 형태의 손상없이 플레이트를 제작할 수 있고, 한천겔 조성분의 유해성분에 대한 인체 노출위험성으로 최소화할 수 있어 한천겔침강반응 검사법의 검사효율을 높이는 데 있다.
한천겔침강반응법, 질병검사, 검사플레이트, 진공흡입장치-
139.发明公开소 ＩｇＧ의 Ｆｃ 도메인의 세포 표면 발현용 벡터, 상기 벡터에 의해 형질전환된 숙주세포 및 상기 숙주세포를 이용한 소 질병 관련 바이러스에 대한 백신의 제조방법 有权BOVINE IGG FC DOMIAN的细胞表面表达载体，用载体转化的宿主细胞和使用宿主细胞与CAT病毒相关的病毒的疫苗的制造方法
公开(公告)号：KR1020100035054A
公开(公告)日：2010-04-02
申请号：KR1020080094356
申请日：2008-09-25
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A vector for expressing IgG Fc domain on cell surface is provided to easily produce a vaccine having excellent immunogenicity to virus relating to bovine diseases. CONSTITUTION: A vector for expressing bovine IgG Fc domain contains a gene encoding the IgG Fc domain and gene encoding transmembrane domain of bovine transferring receptor. The gene encoding the transmembrane domain of bovine transferring receptor comprises a sequence of sequence number 5. The gene encoding Fc domain is a gene encoding hinge, CH2 and CH3. A method for manufacturing the vaccine to bovine disease-relating virus comprises: a step of infecting bovine disease-relating virus to a host cell using the expression vector to proliferate the virus; and a step of manufacturing the live vaccine or inactivated vaccine. The virus is bovine viral diarrhea virus (BVDV), ephemeral fever virus (BEF), bovine corona virus (BCV), bovine rotavirus (BoRotaV), bovine rhi notracheitis virus (IBRV), foot-and-mouth disease virus (FMDV), bovine parainfluenza virus (PI-3), blue tongue virus (BTV), bovine leukemia virus (BLV), cattle respirator syncytia virus (BRSV), akabane virus (AKV) or Ibaraki virus (IBV).
Abstract translation: 目的：提供用于在细胞表面上表达IgG Fc结构域的载体，以容易地产生对与牛疾病有关的病毒具有优异免疫原性的疫苗。 构成：用于表达牛IgG Fc结构域的载体含有编码IgG Fc结构域的基因和编码牛转移受体的跨膜结构域的基因。 编码牛转移受体的跨膜结构域的基因包含序列号5的序列。编码Fc结构域的基因是编码铰链，CH2和CH3的基因。 用于制造与牛疾病相关的病毒的疫苗的方法包括：使用表达载体将病毒相关病毒感染宿主细胞以增殖病毒的步骤; 以及制造活疫苗或灭活疫苗的步骤。 该病毒是牛病毒性腹泻病毒（BVDV），短暂热病毒（BEF），牛冠状病毒（BCV），牛轮状病毒（BoRotaV），牛病毒性气管炎病毒（IBRV），口蹄疫病毒（FMDV） 牛副流感病毒（PI-3），蓝舌病毒（BTV），牛白血病病毒（BLV），牛呼吸器合胞病毒（BRSV），阿卡巴因病毒（AKV）或茨巴病毒（IBV））。
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公开(公告)号：KR1020090117448A
公开(公告)日：2009-11-12
申请号：KR1020080043496
申请日：2008-05-09
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A23K10/30 , A23K20/195
Abstract: PURPOSE: An antioxidant for supplementing feed using Forsythiae fructus extract and a preparation method for the Forsythiae Fructus extract are provided to show improved daily gain, feed intake, and feed efficiency compared to a control group. CONSTITUTION: An antioxidant for supplementing feed contains Forsythiae fructus extract. A method for preparing Forsythiae fructus extract comprises the following steps of: washing 100g Forsythiae fructus with water to remove dust; placing the washed Forsythiae fructus in a white bag; adding 1,000ml distilled water into the white bag to soak for 20-30 minutes; boiling down the soaked Forsythiae fructus for 150 minutes and obtaining an extract; adding another 800ml distilled water and boiling down for 150 minutes to obtain the extract; adding another 700ml distilled water and boiling down for 150 minutes to obtain the extract; collecting the extracts and concentrating until the volume becomes 100ml; and freeze-drying the concentrate.
Abstract translation: 目的：提供使用连翘果提取物补充饲料的抗氧化剂和连翘果提取物的制备方法，以显示与对照组相比改善的日增重，进食量和饲料效率。 构成：补充饲料的抗氧化剂含有连翘果提取物。 连翘果提取物的制备方法为：用水洗涤连翘100份，除去灰尘; 将洗涤的连翘属植物置于白色袋中; 在白色袋中加入1000ml蒸馏水浸泡20-30分钟; 煮熟连翘150分钟，得到提取物; 加入800ml蒸馏水，煮沸150分钟，得到提取物; 再加入700ml蒸馏水，煮沸150分钟，得到提取物; 收集提取物并浓缩直到体积变成100ml; 并冷冻干燥浓缩物。
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